LOCUS       sequence-349722-        2983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Candida optimized monomeric eGFP with RIPLING linker sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ennis CL, Hernday AD, Nobile CJ
  TITLE     A Markerless CRISPR-Mediated System for Genome Editing in Candida 
            auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
  JOURNAL   Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3.
  PUBMED    34730409
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2021-12-22"; volume: "9(3):e0182021. doi"; pages: "
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3"
COMMENT     Sequence Label: pCE1
FEATURES             Location/Qualifiers
     source          1..2983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..186
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        complement(join(1005..1011,1012..1029,1030..1035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1050..1071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1188..1205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1359..1947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1439..1458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2118..2909,2910..2978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2741..2760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2979..2983,1..100))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
       61 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
      121 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      181 cgaggccctt tgacggcacg gccacgcgtt taaaccgccc ggataccagg tttaattaat
      241 gggatgagta aaggagagga acttttcacc ggagtagtgc caattttggt cgagttggac
      301 ggagatgtta atggacataa gttttctgtg tcaggggagg gagaaggtga tgccacatac
      361 ggtaaattga cattaaagtt tatctgtact accggaaagt tacctgtacc ttggccaact
      421 cttgtaacaa cattcgggta tggagtccaa tgtttcgctc gatacccaga ccacatgaag
      481 caacacgatt ttttcaagtc agctatgcct gaagggtatg tccaggaacg aaccatattt
      541 tttaaggatg acggtaatta taagacacga gctgaagtca agtttgaggg ggacaccttg
      601 gtcaatcgaa tcgaattaaa agggatcgac tttaaggaag acggaaatat attggggcat
      661 aaattagagt acaattataa ttcccataac gtgtatatca tggccgataa gcaaaagaac
      721 gggataaaag tcaacttcaa gattagacac aacatagagg atgggtctgt tcaattggct
      781 gatcattatc agcaaaacac cccaatcggg gacgggcctg tattgcttcc agataatcac
      841 tatctttcca cccaatcaaa attgtcaaaa gatcctaatg agaagcgtga tcatatggtc
      901 cttttagaat ttgtaaccgc cgccgggatt actcatggta tggacgaatt gtataaatag
      961 cggtgtttaa accccagcgc ctggcgggcg ctcacaattc cacacaacat acgagccgga
     1021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2101 taaacttggt ctgacagtta ccaatgcttg atcagtgagg cacctatctc agcgatctgt
     2161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2941 aagggaataa gggcgacacg gaaatgttga atactcatac tct
//
