LOCUS       sequence-349725-        9127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. auris LEUpOUT HYG marker CAS9 expression construct. Use with 
            pCE41 gRNA expression construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Ennis CL, Hernday AD, Nobile CJ
  TITLE     A Markerless CRISPR-Mediated System for Genome Editing in Candida 
            auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
  JOURNAL   Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3.
  PUBMED    34730409
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2021-12-22"; volume: "9(3):e0182021. doi"; pages: "
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3"
COMMENT     Sequence Label: pCE38
FEATURES             Location/Qualifiers
     source          1..9127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(184..288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     356..374
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1961..6064
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1961..6064
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6077..6097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6101..6121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6125..6145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6146..6211
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      6276..6523
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6292..6310)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(join(7337..7343,7344..7361,7362..7367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7382..7403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7520..7537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7691..8279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7771..7790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8450..9127,1..114,115..183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9073..9092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
       61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      361 gcgtatcacg aggccctttg acggcacggc cacgcgttta aaccgccttt tcaaatggtt
      421 cagaaaaata ttgttcttct tcctggtgac cacgtgggca ccgaggttgt aaacgaggcc
      481 gtcaaggttt tgaaagccat tgaggaagca acgtctcaag atatcaaatt caatttttca
      541 acccatctaa ttggtggtgc agccattgac aagaccggag tgcctttgcc cgatgaagcc
      601 ctcgaggctg ccaaaaagtc agacgctgtc ttgttgggag ccgttggtgg ccccaaatgg
      661 ggtactggtg ccgttagacc agagcagggt cttctcaaga tcagaaagga attgggcctt
      721 tatgcgaact tgagaccttg caactttgca tctgactcgc ttttggacct ttctccattg
      781 aaggccagtg ttgtcaaagg aaccaatttc accgtggtgc gtgaattggt cggtggtatc
      841 tactttggtg agagacaaga acaggaagag tctgccgacg gtcagagcgc ttgggacacc
      901 gagaagtaca gtgtctctga agttgaaaga atcaccagaa tggctgcctt catggctttg
      961 cagcacaacc ctcctttgcc catctggtcc ttagacaagg ccaacgtgct tgcctcatct
     1021 agattgtgga gaaagacggt cgacaaggtg atgagcgagg agttccctca attgaccatc
     1081 aaccaccagc tcattgactc tgctgccatg attttggtgc agagcccaac taaattgaac
     1141 ggtgttgtga tcacgtcaaa catgaccaat cacaagactt acctgaagac ctgactcaac
     1201 gcaatcgcca gtcccgggtt tcctggctca cgggcccctc cactacgctg atattttgtc
     1261 aattaaccag gagtctaccc tagtgcgcat tgtcgaaggg agtggaaagc cagggaaaaa
     1321 aaacgtatag tgcggtcccc gtcgacccga gacgacaagg cacccaggtg agtcaggctc
     1381 aaatgcatga aaaattatga aaagtggaaa atcgatggcc ctcaggtgaa ggttatcaaa
     1441 acctcatcag ctcgtcgctc aattgctaaa aatcaactga aacagcgtcc cagtggtgtc
     1501 accccacggt tgtttcaggg ctgcggtgga tcacatggcg actccctttg gcccgtaggg
     1561 tgcgatccgt cagtgcccgg ggccctctgc caggattcta cgcgcattgc aactccgagg
     1621 ccgataccct ttgaccagtg gtcaattcgt cacaattggc aacattttca tgatttggca
     1681 gcttcacgtt gaacaattga accagcagaa tccaggaaac gagtttccgt tgtgaatgac
     1741 cctttgtgtg tgcaaataat catggtggct ccctaatggc ataccctcag tcacctggag
     1801 gaattgctcg ctcgagtctc ctgaggtgtc ctcctgcctg tggaaaaagc ggccaggtgt
     1861 ggtccaccgt tggaacagct tgctgtataa ataccacagg tgcgcacgcc ccaggggcct
     1921 tttttttttt tctcctccct atccaaactt aattttcatc atggataaaa agtatagtat
     1981 tggtttagat attggtacta actctgtggg ttgggcagtt atcactgacg aatataaagt
     2041 tccatcaaag aaatttaagg tgttaggtaa cactgacaga cactcaataa aaaagaatct
     2101 tatcggtgct cttttgttcg actccggtga aactgccgag gctacacgtt taaaaagaac
     2161 agcaagaaga agatataccc gtagaaaaaa tagaatatgt tatttacaag aaatcttttc
     2221 taatgaaatg gctaaagttg atgattcctt tttccataga ttggaagagt catttttggt
     2281 tgaagaagac aaaaagcatg agagacatcc aatctttggg aatatagttg atgaagtggc
     2341 ttaccatgaa aaatatccta ccatttatca tttaagaaag aaattggtag attcaactga
     2401 taaagctgac cttagattaa tctatttagc acttgcccat atgattaaat ttagaggtca
     2461 ttttttgatt gaaggtgatt tgaacccaga taattctgac gtggataaat tatttattca
     2521 attagtccaa acctacaacc aattatttga ggaaaatcca attaatgcta gtggtgtcga
     2581 tgccaaagct atattatcag ccagattatc aaaatctaga cgtttggaaa atttgattgc
     2641 ccaattgcca ggagaaaaaa agaatggatt atttggaaac ttgatcgcat tatcattggg
     2701 tttgacacca aattttaaat ctaattttga tttagctgaa gatgctaaat tacaattatc
     2761 aaaagacacc tatgacgacg atttggacaa tttacttgct caaattggtg atcaatatgc
     2821 agatttgttc ttagctgcta aaaacttatc tgatgctatt ttgttgtctg atattttgag
     2881 agtgaacaca gaaataacca aagctccatt atcagcatct atgatcaaac gttatgatga
     2941 acaccatcag gatttgactt tattgaaagc tttggtgaga caacaattgc cagagaagta
     3001 taaagaaatc tttttcgatc aatctaaaaa cgggtatgca ggttatattg atgggggtgc
     3061 ctcccaagag gaattttaca aatttataaa acctatttta gaaaagatgg atgggactga
     3121 ggaacttttg gtcaaattga acagagaaga tttgttacgt aaacagagaa cttttgataa
     3181 tggtagtata cctcaccaaa ttcatttggg tgagttgcat gcaattttaa gaagacaaga
     3241 agatttttat ccatttttaa aagataatag agaaaaaatc gagaaaattt taacctttag
     3301 aattccatac tatgttgggc ctttggctag aggtaattca agatttgcct ggatgacacg
     3361 taaatcagaa gaaactatta ccccttggaa ttttgaagag gttgttgata aaggagcatc
     3421 agcacagagt tttattgaaa gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt
     3481 tttaccaaaa cattccttgt tatacgaata ttttactgtt tacaatgaac ttacaaaggt
     3541 taaatatgtt actgaaggta tgcgtaagcc agccttttta tctggagaac agaaaaaggc
     3601 aatagttgat ttattgttta aaacaaatag aaaagttact gttaaacaat taaaagaaga
     3661 ttactttaag aaaattgaat gttttgattc agttgaaatc agtggtgttg aagacagatt
     3721 taatgctagt ttaggaactt accatgattt acttaaaatt atcaaagata aagatttctt
     3781 ggataacgaa gaaaatgaag acattttaga agacattgtt ttaaccttaa ctttattcga
     3841 agatagagag atgattgaag aacgtttgaa gacttatgca catttgtttg acgataaagt
     3901 gatgaaacag ttgaaaagaa gacgttatac tggatggggt agattgtctc gtaaattgat
     3961 caatggaatt agagataaac aaagtggtaa aactatcttg gactttttga aatctgacgg
     4021 atttgctaat agaaatttca tgcaattgat ccacgacgat agtttgacat ttaaagaaga
     4081 catccaaaag gcccaagtga gtgggcaagg tgattcatta catgaacata ttgcaaattt
     4141 agccggatct cctgctatta agaaagggat attacaaact gttaaagttg tggatgaatt
     4201 agtgaaagta atgggaagac ataaacctga aaacattgtc attgagatgg caagagaaaa
     4261 tcaaactaca caaaaaggac agaaaaatag tagagaacgt atgaaaagaa tagaagaggg
     4321 tattaaagaa ttgggtagtc aaatattgaa agaacaccca gtggaaaata cccagttgca
     4381 aaatgaaaaa ttatatcttt actaccttca aaatggacgt gatatgtatg ttgatcagga
     4441 attagatata aatagacttt cagattatga tgtagatcat atagttccac aatctttctt
     4501 gaaagatgat tccatagaca ataaagtatt aactagaagt gataaaaata gaggtaaaag
     4561 tgataatgtc ccaagtgagg aagtcgtcaa aaagatgaaa aattactggc gtcaactttt
     4621 gaatgctaaa ttaattactc aaagaaaatt tgataatttg actaaagcag aaagaggtgg
     4681 gctttctgaa ttagataaag ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat
     4741 tactaaacat gttgcccaaa ttttagattc ccgtatgaac actaagtatg acgaaaatga
     4801 taagttaata cgtgaggtta aagtcattac tttaaaatca aaacttgtct ctgatttcag
     4861 aaaggatttc caattctata aagttagaga aattaataat tatcatcatg ctcatgatgc
     4921 atatttgaat gctgtagttg gaactgcttt aatcaagaaa taccctaaat tagaatctga
     4981 atttgtatat ggtgattaca aagtctatga tgttagaaag atgattgcta aatcagaaca
     5041 agaaattggt aaagctacag ctaaatactt cttttactct aacattatga atttctttaa
     5101 aacagaaatt actttggcaa acggtgaaat tagaaaaaga cctcttattg aaacaaatgg
     5161 tgagactgga gagatagttt gggacaaagg gcgtgatttc gctactgtta gaaaagtttt
     5221 atcaatgcca caagttaaca ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa
     5281 agaaagtatt ttgcctaaaa gaaatagtga taaattgatt gccagaaaaa aggattggga
     5341 tccaaagaaa tatggtggtt tcgactcacc aaccgtagcc tattctgttt tggttgtggc
     5401 aaaggttgaa aagggtaaaa gtaaaaagct taaatcagta aaagaacttt tgggtattac
     5461 aataatggaa agaagttcct ttgaaaagaa ccctattgat tttttggaag ctaaaggtta
     5521 taaggaagta aagaaggact taataatcaa attgcctaaa tattctttat ttgaattaga
     5581 aaatgggaga aaaagaatgt tggcttctgc tggagaattg caaaagggta atgaattagc
     5641 attgccttcc aaatatgtta acttcttgta tttagcttca cactatgaaa agttgaaagg
     5701 gtcaccagaa gataacgagc aaaaacaatt atttgttgaa caacacaaac actacttaga
     5761 tgagattata gaacaaatta gtgaattcag taaaagagtg atattagctg atgcaaattt
     5821 agataaagtt ttgtcagcct ataacaaaca tagagataag ccaattagag aacaagcaga
     5881 aaacattatt cacttattta cccttaccaa tttaggagca cctgctgctt tcaagtattt
     5941 tgatacaaca attgatcgta aaagatatac ctcaacaaaa gaagtcttag acgccacctt
     6001 aattcatcaa tcaatcactg gattgtatga gacaagaatt gatttgtctc aattgggtgg
     6061 tgatgaaggg gctgatccta agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt
     6121 ggatcctaag aaaaagagaa aggttgacta caaagaccat gacggtgatt ataaagatca
     6181 tgacatcgac tacaaggatg acgatgacaa gtgataatga ctgcagagat ccatcgacct
     6241 gccgccaagc taattccggg cgaatttctg tcgagtcatg taattagtta tgtcacgctt
     6301 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     6361 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     6421 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     6481 cttgctttag aaggttttgg gacgctcgaa ggctttaatt tgcggccatg aaaaaacctg
     6541 aattgactgc cacttccgta gagaagtttc ttatcgagaa gtttgattca gtatctgatt
     6601 tgatgcagtt atctgaggga gaggagagtc gtgcattctc cttcgacgtg ggtggacgtg
     6661 ggtacgtatt gcgtgttaac tcctgcgctg acgggttcta taaagaccgt tatgtgtatc
     6721 gtcacttcgc ctcagcagct cttcctatac ctgaggtgct tgatatcggg gaattttctg
     6781 agagtttaac ctactgtata agtcgaagag cacaaggggt tactttacaa gacttgccag
     6841 aaaccgaatt accagccgtg cttcagccag tagctgaggc tatggacgca atcgctgcag
     6901 ccgatttaag tcaaacatca ggtttcggtc cttttgggcc acaagggatc ggtcaatata
     6961 ctacctggcg tgattttatt tgcgccatag cagatcctca cgtgtaccac tggcaaaccg
     7021 tcatggatga cacagtctca gcatccgtgg cccaggcact tgacgaattg atgttatggg
     7081 cagaggattg tcctgaggtg cgacacttgg ttcatgccga ctttgggagt aacaacgtct
     7141 taacagacaa cggacgaata accgctgtga ttgactggtc agaagctatg ttcggtgact
     7201 ctcaatacga agttgccaac atcttcttct ggcgaccttg gcttgcctgc atggaacagc
     7261 aaacacgata cttcgaacga cgacatccag aacggtgttt aaaccccagc gcctggcggg
     7321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8101 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8461 tgatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8761 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9121 aacgttc
//
