LOCUS       sequence-349727-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding GLI1 and EmGFP-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GLI1_pcDNA6.2/EmGFP-Bsd
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    677..701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4026..4050)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4076..4117
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4157..4176)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4201..4249
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4451..4879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4538..4557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4748..4769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4894..5381
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4929..4952)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5314..5333
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5395..5442
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(5461..5463,5464..5466,5467..6177)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5506..5527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5767..5786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6114..6135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6178..6573
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6731..6852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6768..6787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6822..6841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6901..6917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6901..6917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6914..6936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6925..6941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6949..6955,6956..6973,6974..6979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6994..7015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7132..7149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7303..7891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7383..7402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8062..8853,8854..8922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8685..8704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8923..9027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9102..9121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag ctatcaacaa gtttgtacaa aaaagttggc accatgttca actcgatgac
      721 cccaccacca atcagtagct atggcgagcc ctgctgtctc cggcccctcc ccagtcaggg
      781 ggcccccagt gtggggacag aaggactgtc tggcccgccc ttctgccacc aagctaacct
      841 catgtccggc ccccacagtt atgggccagc cagagagacc aacagctgca ccgagggccc
      901 actcttttct tctccccgga gtgcagtcaa gttgaccaag aagcgggcac tgtccatctc
      961 acctctgtcg gatgccagcc tggacctgca gacggttatc cgcacctcac ccagctccct
     1021 cgtagctttc atcaactcgc gatgcacatc tccaggaggc tcctacggtc atctctccat
     1081 tggcaccatg agcccatctc tgggattccc agcccagatg aatcaccaaa aagggccctc
     1141 gccttccttt ggggtccagc cttgtggtcc ccatgactct gcccggggtg ggatgatccc
     1201 acatcctcag tcccggggac ccttcccaac ttgccagctg aagtctgagc tggacatgct
     1261 ggttggcaag tgccgggagg aacccttgga aggtgatatg tccagcccca actccacagg
     1321 catacaggat cccctgttgg ggatgctgga tgggcgggag gacctcgaga gagaggagaa
     1381 gcgtgagcct gaatctgtgt atgaaactga ctgccgttgg gatggctgca gccaggaatt
     1441 tgactcccaa gagcagctgg tgcaccacat caacagcgag cacatccacg gggagcggaa
     1501 ggagttcgtg tgccactggg ggggctgctc cagggagctg aggcccttca aagcccagta
     1561 catgctggtg gttcacatgc gcagacacac tggcgagaag ccacacaagt gcacgtttga
     1621 agggtgccgg aagtcatact cacgcctcga aaacctgaag acgcacctgc ggtcacacac
     1681 gggtgagaag ccatacatgt gtgagcacga gggctgcagt aaagccttca gcaatgccag
     1741 tgaccgagcc aagcaccaga atcggaccca ttccaatgag aagccgtatg tatgtaagct
     1801 ccctggctgc accaaacgct atacagatcc tagctcgctg cgaaaacatg tcaagacagt
     1861 gcatggtcct gacgcccatg tgaccaaacg gcaccgtggg gatggccccc tgcctcgggc
     1921 accatccatt tctacagtgg agcccaagag ggagcgggaa ggaggtccca tcagggagga
     1981 aagcagactg actgtgccag agggtgccat gaagccacag ccaagccctg gggcccagtc
     2041 atcctgcagc agtgaccact ccccggcagg gagtgcagcc aatacagaca gtggtgtgga
     2101 aatgactggc aatgcagggg gcagcactga agacctctcc agcttggacg agggaccttg
     2161 cattgctggc actggtctgt ccactcttcg ccgccttgag aacctcaggc tggaccagct
     2221 acatcaactc cggccaatag ggacccgggg tctcaaactg cccagcttgt cccacaccgg
     2281 taccactgtg tcccgccgcg tgggcccccc agtctctctt gaacgccgca gcagcagctc
     2341 cagcagcatc agctctgcct atactgtcag ccgccgctcc tccctggcct ctcctttccc
     2401 ccctggctcc ccaccagaga atggagcatc ctccctgcct ggccttatgc ctgcccagca
     2461 ctacctgctt cgggcaagat atgcttcagc cagagggggt ggtacttcgc ccactgcagc
     2521 atccagcctg gatcggatag gtggtcttcc catgcctcct tggagaagcc gagccgagta
     2581 tccaggatac aaccccaatg caggggtcac ccggagggcc agtgacccag cccaggctgc
     2641 tgaccgtcct gctccagcta gagtccagag gttcaagagc ctgggctgtg tccatacccc
     2701 acccactgtg gcagggggag gacagaactt tgatccttac ctcccaacct ctgtctactc
     2761 accacagccc cccagcatca ctgagaatgc tgccatggat gctagagggc tacaggaaga
     2821 gccagaagtt gggacctcca tggtgggcag tggtctgaac ccctatatgg acttcccacc
     2881 tactgatact ctgggatatg ggggacctga aggggcagca gctgagcctt atggagcgag
     2941 gggtccaggc tctctgcctc ttgggcctgg tccacccacc aactatggcc ccaacccctg
     3001 tccccagcag gcctcatatc ctgaccccac ccaagaaaca tggggtgagt tcccttccca
     3061 ctctgggctg tacccaggcc ccaaggctct aggtggaacc tacagccagt gtcctcgact
     3121 tgaacattat ggacaagtgc aagtcaagcc agaacagggg tgcccagtgg ggtctgactc
     3181 cacaggactg gcaccctgcc tcaatgccca ccccagtgag gggcccccac atccacagcc
     3241 tctcttttcc cattaccccc agccctctcc tccccaatat ctccagtcag gcccctatac
     3301 ccagccaccc cctgattatc ttccttcaga acccaggcct tgcctggact ttgattcccc
     3361 cacccattcc acagggcagc tcaaggctca gcttgtgtgt aattatgttc aatctcaaca
     3421 ggagctactg tgggagggtg ggggcaggga agatgccccc gcccaggaac cttcctacca
     3481 gagtcccaag tttctggggg gttcccaggt tagcccaagc cgtgctaaag ctccagtgaa
     3541 cacatatgga cctggctttg gacccaactt gcccaatcac aagtcaggtt cctatcccac
     3601 cccttcacca tgccatgaaa attttgtagt gggggcaaat agggcttcac atagggcagc
     3661 agcaccacct cgacttctgc ccccattgcc cacttgctat gggcctctca aagtgggagg
     3721 cacaaacccc agctgtggtc atcctgaggt gggcaggcta ggagggggtc ctgccttgta
     3781 ccctcctccc gaaggacagg tatgtaaccc cctggactct cttgatcttg acaacactca
     3841 gctggacttt gtggctattc tggatgagcc ccaggggctg agtcctcctc cttcccatga
     3901 tcagcggggc agctctggac ataccccacc tccctctggg ccccccaaca tggctgtggg
     3961 caacatgagt gtcttactga gatccctacc tggggaaaca gaattcctca actctagtgc
     4021 ctaattgcca actttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa
     4081 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta
     4141 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4201 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     4261 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4321 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4381 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
     4441 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4501 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4561 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4621 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4681 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4741 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4801 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4861 caaaaattta acgcgaatta tcctcgagct accgggtagg ggaggcgctt ttcccaaggc
     4921 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4981 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     5041 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     5101 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     5161 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     5221 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     5281 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5341 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gactctagac acgtgttgac
     5401 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     5461 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5521 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5581 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5641 ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     5701 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5761 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5821 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5881 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     5941 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     6001 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6061 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6121 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggcc
     6181 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     6241 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     6301 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     6361 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     6421 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     6481 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     6541 ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg
     6601 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6661 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6721 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6841 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     6901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8401 gtcgtttggt atggcttcat tcagcttcgg ttcccaacga tcaaggcgag ttacatgatc
     8461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9061 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     9121 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     9181 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     9241 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
