LOCUS       sequence-349736-       12908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding DOP1B and EmGFP-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 12908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: DOP1B_pcDNA6.2/EmGFP-Bsd
FEATURES             Location/Qualifiers
     source          1..12908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..77
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        139..186
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(205..207,208..210,211..921)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(250..271)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(511..530)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     858..879
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             922..1317
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1475..1596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1512..1531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1566..1585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1645..1661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1658..1680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1669..1685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1693..1699,1700..1717,1718..1723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1738..1759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1876..1893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2127..2146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2806..3597,3598..3666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3429..3448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3667..3771)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3846..3865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4037..4416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4417..4620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4571..4591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4665..4684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4665..4683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4713..4737
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11677..11701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11727..11768
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(11808..11827)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11852..11900
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12102..12530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12189..12208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12399..12420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(12545..12908,1..125)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12580..12603)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
       61 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
      121 tttcgactct agacacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
      181 tacgacaagg tgaggaacta aaccatggtg agcaagggcg aggagctgtt caccggggtg
      241 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      301 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      361 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
      421 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      481 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      541 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      601 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
      661 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
      721 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      781 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      841 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      901 ggcatggacg agctgtacaa ggccaagcct ttgtctcaag aagaatccac cctcattgaa
      961 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     1021 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     1081 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     1141 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     1201 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     1261 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     1321 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     1381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     1441 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     1501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     1621 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct tcggttccca
     3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3781 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     3841 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     3901 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     3961 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     4021 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     4081 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     4141 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     4201 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     4261 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4321 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4381 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     4441 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4501 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     4561 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     4621 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
     4681 agggagaccc aagctggcta gttaagctat caacaagttt gtacaaaaaa gcaggctccg
     4741 cggccgcccc cttcaccatg gatccagaag agcaggagct cttaaatgat tacagataca
     4801 gaagctactc ttcagtgatt gaaaaggctt tgagaaattt tgagtcctcg agtgaatggg
     4861 cggatctcat atcttcactt ggcaaactca acaaggctct tcagagtaac ctgaggtact
     4921 ccttgttgcc aagacggctc ctcatcagca aaagattagc tcagtgtttg caccctgccc
     4981 tgcccagtgg tgtccactta aaagctctgg aaacctacga gattatcttt aaaatcgtgg
     5041 ggaccaaatg gctggccaag gacttgtttc tgtacagctg cgggttattt cctctcctgg
     5101 cacacgcggc ggtgtcggtg aggccggtgc tgctcaccct gtacgagaag tacttcctcc
     5161 cactgcagaa gctgctcctg cccagtctgc aggccttcat cgtgggcctg ctgcccggcc
     5221 ttgaagaggg ctccgagatc tccgacagaa cggatgctct gctcctgaga ctgtcgctgg
     5281 tggttggcaa agaggtgttt tacaccgccc tctgggggag cgtcctggcc agcccgtcca
     5341 tccgcctccc tgcctcagtc ttcgtggtgg gccacatcaa cagggatgcc cccggccggg
     5401 agcagaagta catgctgggg accaatcacc aactcacggt gaagtctttg cgtgcctccc
     5461 tgttggactc aaatgttctt gtgcaaagaa ataatctgga aatcgttctg tttttcttcc
     5521 cattttatac ctgtctggat tccaatgaga gagccatccc cctcctcaga tctgacatcg
     5581 tgcgcattct ctcagccgcc acccagaccc tactgagaag ggacatgtcc ctgaacagaa
     5641 gactgtatgc atggttacta ggctcagaca taaaaggaaa taccgttgtg ccagaatctg
     5701 aaatctcaaa ttcttatgaa gaccagtcgt cttatttttt tgaaaaatac tccaaggatc
     5761 ttttagttga gggtttggct gagatattgc atcagaagtt catagatgct gacgtggagg
     5821 aacgccatca tgcatacctg aagccttttc gcgtcctcat cagtctgctt gacaagccag
     5881 aaatagggcc tcaagtggtt gggaatttgt ttctcgaagt catcagggcc ttttattctt
     5941 actgcagaga tgcccttggc tctgatctta aacttagcta cacccagagt ggaaattcgc
     6001 tgataagtgc aatcaaggaa aacagaaatg cctctgagat tgtcaaaacg gtaaatttgc
     6061 tgataacttc tctaagcaca gactttctct gggattatat gacaaggtgt tttgaggaat
     6121 gctttagacc agtgaagcag cgttacagcg tgaggaacag cgtcagccct ccccccacgg
     6181 tctcggagct ctgcgccctc ctggtcttcc tgctggatgt cattcctttg gaactttact
     6241 ctgaggtgca aacccagtat ctccctcagg tgctcggctg cctggtgcag cctcttgctg
     6301 aggacatgga ggccttaagt ttacctgaac tcacgcatgc cttgaagacg tgtttcaagg
     6361 tgctcagcaa agtccagatg cctccttcct acctcgacac ggagtccacc agcggaacct
     6421 cgagtccagt aaaaggtgaa aacggcaaaa taattttgga aacaaaggca gtgattcccg
     6481 gtgacgaaga tgcttcgttt ccccctctga agtctgagga cagtgggatc gggctcagtg
     6541 cctcgtcacc ggagctctct gagcacttga gggttcctcg agtttctctg gaaagggacg
     6601 acgtttggaa gaagggcggg agcatgcaga ggacgtttct ttgcatccaa gagctaatcg
     6661 ccaactttgc cagcaagaac atttttggag tacagctgac agcgtcagga gaagaaagca
     6721 agtccgagga gcctgcaggg aagagggaca gggatgggac gcagagcctg gcagccaatg
     6781 attccagcag gaagaactct tgggagccca agcccatcac tgtgcctcag ttcaagcaga
     6841 tgctgtcaga cttgttcaca gcacgagggt ctccattcaa gacaaaaagt tcagagtcac
     6901 catcgtcttc gcccagcagc cctgccagga aaaacggggg agaatgggat gttgagaagg
     6961 tggtcattga cctggggggt tccagggagg aacgcaggga ggcctttgcc gccgcctgcc
     7021 acctgctgct ggattgtgcc actttccctg tctacctgtc cgaggaagag accgagcagc
     7081 tctgtgcaac gctcttccag ctgccaggag ccggtgattc cagttttcca tcttggctga
     7141 agtccctcat gactatttgc tgctgtgtga ctgactgcta cctccagaac gtggccattt
     7201 ccactctgct ggaagtgata aaccattccc agtccctggc gcttgtcatt gaagacaaga
     7261 tgaaacgcta taagagctct ggacacaacc ctttttttgg caagctgcag atggtgacgg
     7321 ttcctcccat tgctccaggg atattgaaag tcattgcaga gaaaacagat ttctatcaga
     7381 gggtggctcg cgtgctttgg aatcagctga acaaagagac ccgggagcat cacgtcacct
     7441 gcgtagaatt gttctaccgg ctgcactgcc tggcccctac ggccaacatc tgcgaggaca
     7501 tcatctgcca tgccctcctg gaccctgaca agggaacaag gctggaagct ctgtttagat
     7561 tttccgtgat ctggcatctg acaagagaga tccaaggcag tcgagtaaca tctcacaatc
     7621 gctcctttga taggtccttg tttgtcgtgc tggacagcct ggcctgcacg gatggtgcca
     7681 tcggtgcggc agcccagggc tggctggtgc gtgcgctctc cctcggggac gtggctcgca
     7741 tcctcgaacc cgtgctcctg ctgctgctgc agccaaaaac ccagagaacc tccatccact
     7801 gcctcaagca ggagaactcg gccgatgact tgcaccgttg gtttaacagg aagaaaacct
     7861 ctttcagaga ggcatgcgca gtgcccgagc ctcaggagag cggctctgaa gagcacctgc
     7921 ctctgagcca gttcaccaca gtggaccgtg aagccatttg ggccgaagtg gagaaggagc
     7981 ccgagaagta cccgctgcga ggcgagctga gcgaggaaga gctgccctac tacgtggagc
     8041 ttccagacag gacggcccac ggcgccccgg acagcagcga gcacaccgag tctgcagata
     8101 caagctcctg ccacacggac agcgagaaca cgtcctcctt ctcctcccct tcccacgacc
     8161 tgcaggagct gagcaacgaa gagaactgct gtgcacccat ccccatgggg ggcagggcgt
     8221 accccaagcg ctcggccctg ctggcggcct tccagtcaga aagcttcaag gctggggcca
     8281 agttaagcct ggtgcgggtg gactcggaca agacgcaggc ttctgagtcg ttctccagcg
     8341 acgaggaggc ggacttggag ctccaggccc tcaccacatc caggctgcta aagcagcagc
     8401 gggaaaggca ggaggccgtc gaggccttgt tcaagcacat cctgctctac ctgcagccct
     8461 acgactctcg gcgggtcctc tatgccttct cggtgctgga ggctgtgctc aaaaccaacc
     8521 ctaaggaatt catcgaggct gtgtccagga ctagcatgga taccagctcc accgcgcacc
     8581 tcaacctcat ctccaacctc ctcgctcgcc accaggaggc cctcattggc cagagtttct
     8641 acggaaagct ccagacccag gtccccaacg tgtgccccca ctctctgctc ctggagctgc
     8701 tcacctacct ctgcctgagc ttcctgcgct cctactaccc ttgctatttg aaggtctcgc
     8761 accgagacat tctcggcaac cgggacgtgc aggtcaaaag tgtcgaggtt ttgatcagga
     8821 taatgatgca gctggtctca gtggccaagt cttcggaagg gaagaacgtg gagttcatcc
     8881 acagcttgct gcagaggtgc aaagttcagg agtttgtcct gctctccctg tcggcgtcca
     8941 tgtacacgag ccagaagcgc tacgggctgg ccaccgccca ccacggcagg gccctgccag
     9001 aggacagcct ctttgaggag agtctcatta acttgggtca ggaccagatc tggagtgagc
     9061 acccgctgca gattgagctg ctgaagctgc tgcaggtgct gattgtcttg gaacaccacc
     9121 tgggtcgggc ccatgaggag gcggaaaacc agcccgacct gtcccgggag tggcagagag
     9181 ccctgaactt ccagcaggcc atcagcgccc tgcagtacgt gcagccccac cccctcacct
     9241 cccagggtct tctggtctct gcggtggtga ggggtctgca gcccgcctac ggttacggca
     9301 tgcatccggc ctgggtgagc ttggtcacgc attccttgcc ctacttcgga aagtccctgg
     9361 gctggacggt gacacccttt gttgtccaga tttgcaaaaa cttggatgac ttggtcaagc
     9421 agtatgaaag cgaatctgtg aagctctctg tcagcacaac ctccaagagg gaaaacattt
     9481 ctccagatta tccactcacc cttctagaag gtctaacgac cattagtcat ttttgtcttt
     9541 tggaacaagc caaccaaaac aaaaagacca tggctgcagg tgatcctgcc aacttgagga
     9601 atgccagaaa tgccattttg gaagagctgc ctcgaactgt taacaccatg gcccttctct
     9661 ggaatgttct cagaaaggag gagactcaaa agagacctgt cgatctccta ggggccacga
     9721 agggatcctc ttccgtttac tttaaaacca ccaaaaccat aagacaaaaa attttagact
     9781 tcttaaaccc cttgacggcc catcttgggg ttcagttgac agcggctgtt gcggcagtgt
     9841 ggagcagaaa gaaagcccag cgtcacagta agatgaagat tatcccaacg gcaagtgcat
     9901 cccagctaac ccttgtcgac ttggtgtgtg cactcagcac cctgcagact gacacgctgc
     9961 tgcacctggt gaaggaggtg gtgaagaggc caccccaagt caaagggggt gatgagaaat
    10021 cgcccctagt ggacattcct gtgttgcagt tttgctatgc ttttctccaa aggctcccag
    10081 taccagcctt gcaagagaac ttttcttcac tgttgggagt attgaaagag tctgtacagt
    10141 tgaatctagc cccacctggg tattttctgc ttctcagcat gctgaatgac tttgtaacaa
    10201 gaactcccaa cctggaaaac aagaaggacc aaaaagacct gcaggaaatc actcagaaaa
    10261 tcctagaagc tgtggggaac attgccggct cttccttgga gcaaaccagc tggctaagca
    10321 gaaacctgga agtgaaggcc caacctcagg cctctctaga agaatctgat gctgaggagg
    10381 acctgtatga tgctgctgca gcttcagcaa tggtgtcttc atccgccccg tcggtgtaca
    10441 gcgtgcaagc cctctctctc ctggcagagg tactggcttc cctcctggac atggtttatc
    10501 gaagtgatga gaaggagaaa gctgtgccgt taatctcccg tctgctttac tatgtttttc
    10561 catacttacg caaccacagt gcctacaatg ctcccagctt ccgggctggc gctcagctgc
    10621 tgagctccct gagtggctat gcctacacaa agcgagcctg gaggaaggag gtcctggagc
    10681 tgtttctcga ccccgctttc tttcagatgg atacttcctg tgttcattgg aagtccatta
    10741 ttgaccatct tttgactcat gagaaaacaa tgtttaagga tttaatgaac atgcagagca
    10801 gttctttgaa actattctca agttttgaac agaaagccat gctgttaaag cgccaggctt
    10861 ttgctgtctt cagtggagaa cttgatcaat accaccttta ccttccactg atacaagaac
    10921 gcctgacaga caatctcaga gttggacaga catccatagt tgctgctcag atgtttcttt
    10981 ttttcagagt tttgctgcta agaatatctc ctcaacattt gacttcattg tggccaataa
    11041 tggtctctga attgattcag acattcacac agcttgaaga agatctaaaa gatgaagatg
    11101 agtcattgag aagcaccaac aaagtaaaca gaacgaaagt ttcagtcccg gatgcaaatg
    11161 gaccctcagt gggggagata ccccagagtg aactcatctt gtatttatca gcttgcaaat
    11221 tcttggacac agcgctttct tttccacctg acaagatgcc attatttcaa atttataggt
    11281 gggcatttat tccagaagtg gacacagagg gccctgcctt cctgtcggat gtagaggaga
    11341 atcaccaaga atgcaaaccc cacactgtca ggattctaga acttctaaaa ttaaagtttg
    11401 gggaaatcag tagctctgat gagatcacca tgaagagtga attcccgctt ctgcgccaac
    11461 attctgtttc cagcatcagg cagttgatgc cattcttcat gactctaaat ggtgcattta
    11521 agacccagag acagctgcct gctgatagcc caggaactcc attcttggac tttcctgtca
    11581 cagatagccc aaggatctta aaacaactgg aagaatgcat cgaatatgat tttctggaac
    11641 atccagaatg ttgatgactc aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
    11701 ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
    11761 attctacgcg taccggttag taatgagttt aaacggggga ggctaactga aacacggaag
    11821 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
    11881 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
    11941 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
    12001 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
    12061 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
    12121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    12181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    12241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    12301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    12361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    12421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    12481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt atcctcgagc
    12541 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
    12601 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
    12661 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
    12721 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
    12781 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    12841 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    12901 aggggtgg
//
