LOCUS       sequence-349751-        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for knocking in constitutively expressed PE2-2A-GFP
            into the human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, 
            Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F
  TITLE     Highly efficient generation of isogenic pluripotent stem cell models
            using prime editing.
  JOURNAL   Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208.
  PUBMED    36069759
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-7"; volume: "11:e79208. doi"; pages: " 
            10.7554/eLife.79208"
COMMENT     Sequence Label: AAVS1-SA-neo-CAGGS-PE2-2A-GFP
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     189..208
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             257..277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gap             1281..1293
                     /estimated_length=13
     gap             1308..1319
                     /estimated_length=12
     gap             1322..1352
                     /estimated_length=31
ORIGIN
        1 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
       61 gtctcatcat tttggcaaag aattgaagcc tctgttccac atacacttca ttctcagtat
      121 tgttttgcca agttctaatt ccatcagaag ctgggagctc caccgcggtg gctagctagt
      181 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
      241 aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca gcatcggcct
      301 ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
      361 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
      421 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
      481 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
      541 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
      601 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
      661 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
      721 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
      781 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
      841 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
      901 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
      961 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
     1021 ccccaacttc aanagcaact tcgacctggc cgaggatgcc aactgcagct gagcaaggan
     1081 ncctacgacg acnnctggan aacctgctgg cccanatcgg nnaccagtac nccgacctgt
     1141 tnctggccgc canaanctgt ccnangcnnc ntgntgannn nnntccngan anngaanccn
     1201 naannccaag gcccccngnn nncnnnnnnn nnannaannn annaancccc cnnaactgac
     1261 cngnnnnaan nnnnnngggg nnnnnnnnnn nnnannnaaa aaannttnnn nnnnnnnnna
     1321 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnc
//
