LOCUS       sequence-34976-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia 
            I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, 
            Samuels Y
  TITLE     Mutational and functional analysis reveals ADAMTS18 
            metalloproteinase as a novel driver in melanoma.
  JOURNAL   Mol Cancer Res. 2010 Nov . 8(11):1513-25.
  PUBMED    21047771
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2010 Nov . 8(11):1513-25."
COMMENT     Sequence Label: pCDF1-ADAMTS18 (C638S)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..634)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(118..138)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     615..634
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tttaaatttt gtaatccaga ggttgattgt cgactcaggc accgggcttg cgggtcatgc
       61 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
      121 cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct
      181 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
      241 gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
      301 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
      361 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
      421 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
      481 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
      541 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
      601 tggcgaggcg caccgtgggc ttgtactcgg tcatctagcg taggcgccgg tcacagcttg
      661 gatctgtaac ggcgcagaac agaaaacgaa acaaagacgt agagttgagc aagcagggtc
      721 aggcaaagcg tggagagccg gctgagtcta ggtaggctcc aagggagcgc cggacaaaag
      781 gccccggtct cgacctgagc t
//
