LOCUS       sequence-34977-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia 
            I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, 
            Samuels Y
  TITLE     Mutational and functional analysis reveals ADAMTS18 
            metalloproteinase as a novel driver in melanoma.
  JOURNAL   Mol Cancer Res. 2010 Nov . 8(11):1513-25.
  PUBMED    21047771
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2010 Nov . 8(11):1513-25."
COMMENT     Sequence Label: pCDF1-ADAMTS18 (Q904X)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..642)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(126..146)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     623..642
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 taagtatctt taaattttgt aatccagagg ttgattgtcg actcaggcac cgggcttgcg
       61 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
      121 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
      181 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
      241 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
      301 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
      361 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
      421 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
      481 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
      541 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
      601 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atctagcgta ggcgccggtc
      661 acagcttgga tctgtaacgg cgcagaacag aaaacgaaac aaagacgtag agttgagcaa
      721 gcagggtcag gcaaagcgtg gagagccggc tgagtctagg taggctccaa gggagcgccg
      781 gacaaaggcc cgttctcgac ctgagcttta aacttaccta gacggctgga cgcag
//
