LOCUS       sequence-34978-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia 
            I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, 
            Samuels Y
  TITLE     Mutational and functional analysis reveals ADAMTS18 
            metalloproteinase as a novel driver in melanoma.
  JOURNAL   Mol Cancer Res. 2010 Nov . 8(11):1513-25.
  PUBMED    21047771
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2010 Nov . 8(11):1513-25."
COMMENT     Sequence Label: pCDF1-ADAMTS18 (Q1002X)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..636)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(120..140)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     617..636
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tctttaaatt ttgtaatcca gaggttgatt gtcgactcag gcaccgggct tgcgggtcat
       61 gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg
      121 ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg
      181 ctcggccgcc tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc
      241 gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag
      301 gaggccttcc atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg
      361 gccgatctcg gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac
      421 cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa
      481 gagttcttgc agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt
      541 ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg
      601 ggtggcgagg cgcaccgtgg gcttgtactc ggtcatctag cgtaggcgcc ggtcacagct
      661 tggatctgta acggcgcaga acagaaaacg aaacaaagac gtagagttga gcaagcaggg
      721 tcaggcaaag cgtggagagc cggctgagtc taggtaggct ccaagggagc gccggacaaa
      781 ggcccggtct cgacctgagc tttaaactta cctagacggc ggacgcagt
//
