LOCUS       sequence-349788-        5758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mutant human DGCR8 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Zhang X, Li P, Zhong H, Yang F, Liu F, Yedid G, Zeng Y
  TITLE     Extending the L1 region in canonical double-stranded RNA-binding 
            domains impairs their functions.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2021 Mar 26;53(4):463-471. doi:
            10.1093/abbs/gmab014.
  PUBMED    33751023
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2021-03-26- 26"; 
            volume: "53"; issue: "4"; pages: "463-471"
COMMENT     Sequence Label: pCMV-HA-DGCR8(+6)
FEATURES             Location/Qualifiers
     source          1..5758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        327..706
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        711..914
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     865..885
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             987..1076
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYLYDVPDYA"
     primer_bind     complement(3735..3753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3821..3925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3926..3994,3995..4786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4144..4163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5322..5341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
       61 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
      121 ctcgcgtcgg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
      181 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
      241 ccatgagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
      301 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
      361 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      421 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      481 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      541 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      601 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      661 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg gtgatgcggt
      721 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      781 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      841 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      901 atataagcag agctcgttta gtgaagtcaa gcttaagtga ccagctacag tcggaaacca
      961 tcagcaagag gtcattgttc accatgtacc catacgatgt tcctgactat gcgggctatc
     1021 cctatgacgt cccggactat gcaggatcct atctatatga cgttccagat tacgctgcca
     1081 tggagacaga tgagagcccc tctccgctcc cgtgtgggcc cgcaggagaa gcggtgatgg
     1141 agagccgagc tcgccccttc caagcgctgc cccgtgagca gtctccacca cctcccctgc
     1201 aaacgtccag tggtgcagag gtaatggacg ttggctctgg tggtgatgga cagtccgaac
     1261 tccctgctga ggaccccttc aacttctacg gagcttctct tctctccaaa ggatccttct
     1321 ctaagggccg cctcctcata gacccgaact gtagtggcca cagcccgcgc accgcccggc
     1381 acgcacctgc ggtccggaag ttctcccctg accttaagtt gcttaaggat gtaaagatta
     1441 gcgtgagctt taccgagagc tgcaggagta aggacaggaa ggtgctgtac acaggagcag
     1501 agcgcgacgt gcgggcggag tgcggtctgc tccttagccc tgtcagtggg gacgtgcatg
     1561 cttgtccctt tggcgggagt gttggtgacg gggtaggcat agggggtgag agtgctgata
     1621 agaaggatga ggagaatgag ctggatcagg aaaggagagt ggagtatgca gtgctcgatg
     1681 agttagaaga ttttactgac aatttggagc tagatgaaga aggagcaggc gggttcacgg
     1741 ctaaagcaat cgttcagaga gacagagtgg atgaagaggc cttgaatttc ccctacgagg
     1801 atgactttga caacgatgtg gatgctctgc tggaagaagg cctttgtgcc cccaaaaaga
     1861 ggcgaacaga ggaaaaatat ggcggagaca gcgaccatcc gtccgatgga gagacaagtg
     1921 tgcagccgat gatgaccaag attaaaacag tgctcaaaag tcgtggccgc ccacctacag
     1981 agccgctgcc cgacgggtgg atcatgacat tccataactc tggagtcccg gtgtacctac
     2041 acagagagtc tcgggtggtc acctggtcca ggccatactt cttgggaacg ggaagcatac
     2101 ggaaacacga ccctcctctg agtagcatcc cttgtctgca ttataagaaa atgaaggaca
     2161 acgaggaacg ggagcaaagc agtgacctca cccctagtgg ggatgtgtcc cccgtcaagc
     2221 ccctgagccg atctgcagag ctggagtttc ccctggatga gcctgactct atgggtgctg
     2281 accctgaccc ggggcccccg gacgagaaag acccactagg ggctgaggca gcccctgggg
     2341 ccctggggca ggtgaaggcc aaagtcgagg tgtgcaaaga tgaatccgtt gatctcgagg
     2401 aatttcgaag ctacctggag aagcgttttg actttgagca agttactgtg aaaaaattca
     2461 ggacttgggc tgagcggcgg caattcaatc gggaaatgaa gcggaagcag gcggagtccg
     2521 agaggcccat cttgccagcc aatcagaagc tcattacttt atcagtgcaa gatgcaccca
     2581 caaagaaaga gtttgttatt aaccccaacg ggaaatccga ggtctgcatc ctgcacgagt
     2641 acatgcagcg tgtcctcaag acccttcgga ctgaaggtgt ccgccctgtc tataatttct
     2701 ttgaatgtga gaacccaagt gagccttttg gtgcctcggt gaccattgat ggtgtgactt
     2761 acggatctgg aactgcaagc agcaaaaaac ttgcgaagaa taaagctgcc cgagctacac
     2821 tggaaatcct catccctgac tttgttaaac agacctctga agagaagccc aaagacagtg
     2881 aagaactcga gtattttaac cacatcagca tcgaggactc gcgggtctac gagctgacca
     2941 gcaaggctgg gctgttgtct ccatatcaga tcctccacga gtgccttaaa agaaaccatg
     3001 ggatgaccct tcggactgaa ggtggtgaca cgtctatcaa gtttgaagtg gttcctggga
     3061 aaaaccagaa gagtgaatac gtcatggcgt gtggcaagca cacagtgcgc gggtggtgta
     3121 agaacaagag agttggaaag cagttagcct cacagaagat ccttcagctg ctgcacccac
     3181 atgtcaagaa ctgggggtct ttactgcgca tgtatggccg tgagagcagc aagatggtca
     3241 agcaggagac atcggacaag agtgtgattg agctgcagca gtatgccaag aagaacaagc
     3301 ccaacctgca catcctcagc aagctccaag aggagatgaa gaggctagct gaggaaaggg
     3361 aggagactcg aaagaagccc aagatgtcca ttgtggcgtc cgcccagcct ggcggtgagc
     3421 ccctgtgcac cgtggacgtg tgagttaacg tcgagtgacc ttcagacctt ggcactggag
     3481 gtggcccggc agaagcgcgg catcgtggat cagtgctgca ccagcatctg ctctctctac
     3541 caactggaga actactgcaa ctaggcccac cactaccctg tccacccctc tgcaatgaat
     3601 aaaacctttg aaagagcact acaagttgtg tgtacatgcg tgcatgtgca tatgtggtgc
     3661 ggggggaaca tgagtggggc tggctggagt ggcgatgata agctgtcaaa catgagaatt
     3721 cttgaagacg aaaggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3781 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4141 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4441 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     4501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggc
     4861 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4921 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4981 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5041 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5101 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5161 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5221 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5281 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5341 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5401 ggggggcgga gcctatggaa aaacgccagc aacggagatg cgccgcgtgc ggctgctgga
     5461 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     5521 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
     5581 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     5641 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     5701 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcga
//
