LOCUS       sequence-34979-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia 
            I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, 
            Samuels Y
  TITLE     Mutational and functional analysis reveals ADAMTS18 
            metalloproteinase as a novel driver in melanoma.
  JOURNAL   Mol Cancer Res. 2010 Nov . 8(11):1513-25.
  PUBMED    21047771
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2010 Nov . 8(11):1513-25."
COMMENT     Sequence Label: pCDF1-ADAMTS18 (P1035S)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..635)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(119..139)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     616..635
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tctttaaatt ttgtatccag aggttgattg tcgactcagg caccgggctt gcgggtcatg
       61 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
      121 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
      181 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
      241 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
      301 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
      361 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
      421 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
      481 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
      541 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
      601 gtggcgaggc gcaccgtggg cttgtactcg gtcatctagc gtaggcgccg gtcacagctt
      661 ggatctgtaa cggcgcagaa cagaaaacga aacaaagacg tagagttgag caagcagggt
      721 caggcaaagc gtggagagcc ggctgagtct aggtaggctc caagggagcg ccggacaaag
      781 gcccggtctc gacctgagct tttaaactta ccta
//
