LOCUS       sequence-349810-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA targeting hORAI1 - encodes puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
  TITLE     NKD2 mediates stimulation-dependent ORAI1 trafficking to augment 
            Ca(2+) entry in T cells.
  JOURNAL   Cell Rep. 2021 Aug 24;36(8):109603. doi: 
            10.1016/j.celrep.2021.109603.
  PUBMED    34433025
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-24"; volume: "36(8):109603. doi"; pages: " 
            10.1016/j.celrep.2021.109603"
COMMENT     Sequence Label: pLentiguide-puro_hORAI1sg
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             113..293
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    340..465
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    958..1191
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1376..1420
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1598..1846
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1598..1618
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1769..1788
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1868..1943
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1999..2116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2255..3433
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2486..3424
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3381..3401
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3446..4045
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3446..3465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3942..3962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4061..4649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4114..4134)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4532..4543)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4721..4954
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5032..5153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5069..5088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5123..5142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5193..5328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5348..5367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5349..5367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5377..5394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5377..5393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5386..5408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5535..5990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5622..5641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5832..5853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6016..6120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6121..6189,6190..6981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6339..6358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7152..7740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7641..7660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7894..7911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8028..8049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8064..8069,8070..8087,8088..8094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8102..8118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8107..8129
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8126..8142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8126..8142
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8161..8181
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8163..8181
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(8209..8323,1..112)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
       61 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      301 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      361 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      421 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      481 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      541 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      601 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      661 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      721 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      781 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      841 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
      901 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
      961 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1021 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1081 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1141 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1201 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1261 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1321 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1381 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1441 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1501 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1561 gtttcagacc cacctcccaa ccccgagggg acccagagag ggcctatttc ccatgattcc
     1621 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
     1681 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     1741 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     1801 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggat cggccagagt
     1861 tactccggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     1921 aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat
     1981 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2041 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2101 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     2161 cgagggggcc cgggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     2221 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     2281 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc
     2341 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     2401 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     2461 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     2521 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     2581 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     2641 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     2701 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     2761 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     2821 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     2881 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     2941 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     3001 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     3061 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     3121 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     3181 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     3241 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     3301 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     3361 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     3421 tcaggtgtcg tgacgtacgg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     3481 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3541 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3601 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3661 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3721 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3781 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc
     3841 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     3901 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     3961 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4021 catgacccgc aagcccggtg cctgaacgcg ttaagtcgac aatcaacctc tggattacaa
     4081 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4141 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4201 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4261 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4321 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4381 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4441 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4501 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4561 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4621 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     4681 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4741 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4801 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4861 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4921 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     4981 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     5041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5161 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5221 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5281 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     5341 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     5401 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5461 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5521 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5761 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5821 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5881 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5941 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     6001 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6061 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6481 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6541 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6661 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6841 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6901 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6961 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7021 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7261 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7801 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7921 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7981 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8041 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8101 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     8161 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     8221 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     8281 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gta
//
