LOCUS       sequence-349820-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral 
            vector. Does not encode any fluorescence/resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
  TITLE     NKD2 mediates stimulation-dependent ORAI1 trafficking to augment 
            Ca(2+) entry in T cells.
  JOURNAL   Cell Rep. 2021 Aug 24;36(8):109603. doi: 
            10.1016/j.celrep.2021.109603.
  PUBMED    34433025
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-08-24"; volume: "36(8):109603. doi"; pages: " 
            10.1016/j.celrep.2021.109603"
COMMENT     Sequence Label: FG11F-C-FLAG-hNKD2
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    127..244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        342..1553
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1390..1409
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2893..2919
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             2932..2955
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2983..3571
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3036..3056)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3454..3465)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3574..3590)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3575..3591)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4100..4280
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4306..4323)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4312..4536
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4582..5010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4669..4688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4879..4900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5019..5039)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5024..5353
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5204..5339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5266..5285
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5401..5448
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5467..5841
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5971..6092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6008..6027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6062..6081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6141..6157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6141..6157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6154..6176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6165..6181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6189..6195,6196..6213,6214..6219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6234..6255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6372..6389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6543..7131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6623..6642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7302..8093,8094..8162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7925..7944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8163..8267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8342..8361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8533..8912
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8914..9112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9067..9087
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9130..9310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9357..9482
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9975..10208
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10393..10437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
       61 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
      121 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      181 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      241 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattctag acagttaaca
      301 tctcgagcca taaagatggt taattaaccc acccaagatc tggcctccgc gccgggtttt
      361 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
      421 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
      481 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
      541 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
      601 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
      661 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
      721 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
      781 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
      841 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
      901 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
      961 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     1021 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     1081 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     1141 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     1201 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     1261 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     1321 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     1381 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     1441 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     1501 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     1561 ggtaccgagc tcggatccat ggggaaactg cagtcgaagc acgccgccgc cgcccgcaag
     1621 cggagagaga gcccggaagg ggacagcttc gtggcgtccg cgtacgcgag cggccgcaaa
     1681 ggcgcggagg aagcggagcg gcgcgcgcgg gacaagcagg agctgcccaa tggggacccc
     1741 aaggaggggc ctttccggga ggaccagtgt cccctacagg tggcactccc cgctgagaaa
     1801 gctgagggcc gcgagcaccc gggacaactc ctcagcgcag atgacggaga gagggcagca
     1861 aaccgcgagg gcccgcgagg accgggcggg cagcgcctca acattgacgc actccagtgc
     1921 gatgtctcgg tggaggagga cgaccgccag gagtggacgt tcacgctcta tgactttgac
     1981 aactgcggga aggtcaccag ggaggacatg tccagcctca tgcacaccat ctatgaggtc
     2041 gtggatgcct cggtcaacca ctcctcgggc agcagcaaga ccctccgtgt gaagctaacc
     2101 gtcagccctg agccctccag caagaggaag gagggtcctc ctgctggcca ggaccgggag
     2161 cccacccgtt gcaggatgga gggtgaactg gcagaggagc caagggtggc tgacaggagg
     2221 ttgtctgcac acgtcaggag gcccagtact gacccccagc cctgctcgga gcgggggccc
     2281 tactgcgtgg acgagaacac ggagcgcaga aaccactacc tggacctcgc cgggattgag
     2341 aactacacgt ccagattcgg ccctgggtcc cctcctgtgc aagcaaagca ggagccccag
     2401 ggcagggcct cgcacctcca ggcccggtcc cgctcccagg agccagatac acatgccgta
     2461 caccaccgca ggtcacaggt gctggtggaa cacgtcgtgc cagcctcgga gcctgctgcc
     2521 cgggccctgg acacgcagcc ccggccgaag gggccggaga agcagttcct caagtccccc
     2581 aagggctccg ggaagccgcc tggggtgcca gccagcagca agtccgggaa agccttcagc
     2641 tactacctgc cggccgtcct gccgccccag gcccctcagg acggccacca cctcccgcag
     2701 cccccaccgc caccctacgg ccacaagcgg taccgccaaa agggcaggga gggccactcg
     2761 ccactcaagg ccccacacgc tcagcctgcc acagtggagc acgaggtggt gcgggacctg
     2821 ccgcccacgc cagcaggaga gggctacgcg gtgccagtga tccagcggca cgagcaccac
     2881 caccaccacg agcaccacca ccaccaccac caccaccact tccacccgtc cgactacaag
     2941 gacgacgatg acaagtgaga attcgatatc aagcttatcg ataatcaacc tctggattac
     3001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3361 gtggtgttgt cggggaaatc atcgtccttt ccttggctgt tcgcctgtgt tgccacctgg
     3421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3541 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     3601 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     3661 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     3721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3781 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     3841 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     3901 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     3961 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     4021 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     4081 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4261 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     4321 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4381 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4441 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4501 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4561 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4621 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4741 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4801 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4861 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4921 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4981 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     5041 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5101 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5161 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5221 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5281 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5341 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     5401 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     5461 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     5521 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     5581 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     5641 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     5701 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     5761 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     5821 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     5881 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5941 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6121 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6181 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6241 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6301 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6421 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8281 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8341 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8401 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8461 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     8521 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     8581 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     8641 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     8701 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     8761 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     8821 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8881 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8941 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9001 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9061 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     9121 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9181 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9241 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9301 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9361 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9421 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9481 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9541 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9601 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9661 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9721 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9781 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     9841 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9901 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9961 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    10021 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    10081 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    10141 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    10201 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    10261 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    10321 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    10381 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10441 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10501 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    10561 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgagggga
//
