LOCUS       sequence-34984-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Koushik SV, Vogel SS
  TITLE     Energy migration alters the fluorescence lifetime of Cerulean: 
            implications for fluorescence lifetime imaging Forster resonance 
            energy transfer measurements.
  JOURNAL   J Biomed Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367.
  PUBMED    18601528
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomed 
            Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367."
COMMENT     Sequence Label: CTA
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(170..880,881..883))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGCGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(212..233)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     561..580
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     820..841
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctgcataaca gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      121 gttatctaga tccggtggat cccgggcccg cggtaccgtc gactgcagcc ttgtacagct
      181 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      241 gcttctcgtt ggggtctttg ctcagcttgg actggtagct caggtagtgg ttgtcgggca
      301 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      361 cgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt
      421 cggcggtgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      481 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      541 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      601 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      661 ggtagcgggc gaagcactgc aggccgcagc cgagggtggt cacgagggtg ggccagggca
      721 cgggcagctt gccggtggtg cagatgagct tcagggtcag cttgccgtag gtggcatcgc
      781 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      841 ggatgggcac caccccggtg aacagctcct cgcccttgct cacagatctg a
//
