LOCUS       sequence-34985-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Koushik SV, Vogel SS
  TITLE     Energy migration alters the fluorescence lifetime of Cerulean: 
            implications for fluorescence lifetime imaging Forster resonance 
            energy transfer measurements.
  JOURNAL   J Biomed Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367.
  PUBMED    18601528
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomed 
            Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367."
COMMENT     Sequence Label: VTA
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(93..803)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(135..156)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     484..503
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     743..764
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcggcagtca tggccaccct ctccacctcc ctgttcagga cgcagacaat gttctcaaaa
       61 gtggccgtct tcttctccag gctctctccg gacttgtaca gctcgtccat gccgagagtg
      121 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      181 ttgctcagct tggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      241 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg
      301 tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg
      361 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      421 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      481 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      541 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
      601 tgcaggccgt agccgagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      661 gtgcagatga gcttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      721 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      781 gtgaacagct cctcgccctt gctca
//
