LOCUS       sequence-34986-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Koushik SV, Vogel SS
  TITLE     Energy migration alters the fluorescence lifetime of Cerulean: 
            implications for fluorescence lifetime imaging Forster resonance 
            energy transfer measurements.
  JOURNAL   J Biomed Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367.
  PUBMED    18601528
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomed 
            Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367."
COMMENT     Sequence Label: VTA
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(179..430)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(221..242)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     570..589
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatagctgca taacagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcagttat ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcct
      181 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      241 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctggtagctc aggtagtggt
      301 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      361 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      421 tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      481 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      541 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      601 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      661 tgtggtcggg gtagcgggcg aagcactgca ggccgcagcc gagggtggtc acgagggtgg
      721 gccagggcac gggcagcttg ccggtggtgc a
//
