LOCUS       sequence-349865-        8458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express gRNA expression cassette simultaneously targeting two 
            genes: UL8 and UL29 (EGFP version)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Yin D, Ling S, Wang D, Dai Y, Jiang H, Zhou X, Paludan SR, Hong J, 
            Cai Y
  TITLE     Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic 
            stromal keratitis in mice.
  JOURNAL   Nat Biotechnol. 2021 Jan 11. pii: 10.1038/s41587-020-00781-8. doi: 
            10.1038/s41587-020-00781-8.
  PUBMED    33432198
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jan 11. pii: 10.1038/s41587-020-00781-8. doi: 
            10.1038/s41587-020-00781-8."
COMMENT     Sequence Label: pLV-U6-UL29-egfp-U3-UL8
FEATURES             Location/Qualifiers
     source          1..8458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             599..779
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    826..951
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1444..1677
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1862..1906
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2173..2290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2317..2565
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        2689..3188
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2724..2747)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3121..3140
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3218..3220,3221..3223,3224..3937)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3218..3220,3221..3223,3224..3934)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3263..3284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3524..3543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3871..3892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3961..4549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4014..4034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4432..4443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4670..4918
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     LTR             5052..5232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5261..5279)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5262..5279)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5525..5544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5526..5544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5554..5571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5554..5570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5563..5585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5712..6167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5799..5818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6009..6030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6193..6297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6298..6366,6367..7158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6516..6535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7329..7917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7818..7837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8071..8088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8205..8226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8241..8246,8247..8264,8265..8271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8279..8295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8284..8306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8303..8319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8303..8319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8338..8358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8340..8358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg
      601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      781 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
      841 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
      901 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
      961 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1021 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1081 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1141 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1201 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1261 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1321 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1381 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1441 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1501 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1561 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1621 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1681 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1741 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1801 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1861 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1921 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1981 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2041 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2101 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2161 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2221 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2281 tcaaaatttt atcgatcacg agactagcct cgacgagagg gcctatttcc catgattcct
     2341 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     2401 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     2461 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     2521 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgcgag cgtacacgta
     2581 tcccgtttca gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat
     2641 caacttgaaa aagtggcacc gagtcggtgc tttttttggt cgagtaccgg gtaggggagg
     2701 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     2761 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     2821 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     2881 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     2941 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     3001 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     3061 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     3121 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3181 gcctttcgac ctctagcggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     3241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3661 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3721 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3901 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggcctcgagg gaattccgat
     3961 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4321 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4501 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgggaattc
     4561 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag agggcctatt
     4681 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
     4741 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     4801 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     4861 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
     4921 ggcagccata ccgcgtaagt ttcagagcta tgctggaaac agcatagcaa gttgaaataa
     4981 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gatctgcttt
     5041 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5101 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5161 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5221 aaatctctag cagcatctag ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     5281 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     5341 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     5401 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     5461 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagcctaggg acgtacccaa
     5521 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     5581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5701 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5761 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5821 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5881 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5941 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6001 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6061 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6121 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6841 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6901 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6961 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7021 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7081 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7141 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7201 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7261 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7981 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8041 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8101 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8281 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     8341 attaaccctc actaaaggga acaaaagctg gagctgcaag cttggccatt gcatacgttg
     8401 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgtt
//
