LOCUS       sequence-349867-        8835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one dox-repressible expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Spinelli JB, Rosen PC, Sprenger HG, Puszynska AM, Mann JL, Roessler 
            JM, Cangelosi AL, Henne A, Condon KJ, Zhang T, Kunchok T, Lewis CA, 
            Chandel NS, Sabatini DM
  TITLE     Fumarate is a terminal electron acceptor in the mammalian electron 
            transport chain.
  JOURNAL   Science. 2021 Dec 3;374(6572):1227-1237. doi: 
            10.1126/science.abi7495. Epub 2021 Dec 2.
  PUBMED    34855504
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abi7495"; journalName: "Science"; date: "2021-12-3-
            3"; volume: "374"; issue: "6572"; pages: "1227-1237"
COMMENT     Sequence Label: pCW57.1 tTA UQCRC2
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    162..180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     263..287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      338..347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1736..2246
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2165..2183
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2256..2648
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2658..2711
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2712..3329,3330..3335,3336..3371,3372..3374,3375..3410,
                     3411..3413,3414..3449,3450..3458)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3498..3518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3916..3927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4132..4365
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4437..4558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4474..4493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4528..4547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4598..4733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4733..4804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4805..4873,4874..5665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5023..5042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5836..6424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6325..6344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6578..6595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6712..6733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6748..6753,6754..6771,6772..6778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6786..6802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6791..6813
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6810..6826
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6810..6826
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6845..6865
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6847..6865
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6893..7119
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7120..7300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7347..7472
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7965..8198
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8383..8427
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8640..8757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8811..8835,1..290)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    join(8819..8835,1..2)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
       61 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
      181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
      241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
      301 gctagcatca tcctctagac tttaattaag gatccgcgcc accatgggaa tgaagctact
      361 aaccagagcc ggctctttct cgagatttta ttccctcaaa gttgccccca aagttaaagc
      421 cacagctgcg cctgcaggag caccgccaca acctcaggac cttgagttta ccaagttacc
      481 aaatggcttg gtgattgctt ctttggaaaa ctattctcct gtatcaagaa ttggtttgtt
      541 catcaaggct ggaagcagat acgaagactt cagcaattta ggaaccaccc atttgctgcg
      601 tcttacatcc agtctgacga caaaaggagc ttcatctttc aagataaccc gtggaattga
      661 agcagttggt ggcaaattaa gtgtgaccgc aacaagggaa aacatggctt atactgtgga
      721 atgcctgcgg ggtgatgttg atattctaat ggagttcctg ctcaatgtca ccacagcacc
      781 agaatttcgt cgttgggaag tagctgacct tcagcctcag ctaaagattg acaaagctgt
      841 ggcctttcag aatccgcaga ctcatgtcat tgaaaatttg catgcagcag cttaccggaa
      901 tgccttggct aatcccttgt attgtcctga ctataggatt ggaaaagtga catcagagga
      961 gttacattac ttcgttcaga accatttcac aagtgcaaga atggctttga ttggacttgg
     1021 tgtgagtcat cctgttctaa agcaagttgc tgaacagttt ctcaacatga ggggtgggct
     1081 tggtttatct ggggccaaag caaattatcg aggaggtgaa atccgagaac agaatggaga
     1141 cagtcttgtc catgctgctt ttgtagcaga aagtgctgtc gcgggaagtg cagaggcaaa
     1201 tgcatttagt gttcttcagc atgtcctcgg tgctgggcca catgtcaaga ggggcagcaa
     1261 caccaccagc catctgcacc aggctgttgc caaggcaact cagcagccat ttgatgtttc
     1321 tgcatttaat gccagttact cagattctgg actctttggg atttatacta tctcccaggc
     1381 cacagctgct ggagatgtta tcaaggctgc ctataatcaa gtaaaaacaa tagctcaagg
     1441 aaacctttcc aacacagatg tccaagctgc caagaacaag ctgaaagctg gatacctaat
     1501 gtcagtggag tcttctgagt gtttcctgga agaagtcggg tcccaggctc tagttgctgg
     1561 ttcttacatg ccaccatcca cagtccttca gcagattgat tcagtggcta atgctgatat
     1621 cataaatgcg gcaaagaagt ttgtttctgg ccagaagtca atggcagcaa gtggaaattt
     1681 gggacataca ccttttgttg atgagttgta agggtcgagg ggagaattcg gatccggggt
     1741 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1801 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1861 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1921 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1981 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     2041 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     2101 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     2161 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     2221 accgaatcac cgacctctct ccccagcaat tcaccatggt tcctttgtct caagaagaat
     2281 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     2341 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     2401 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     2461 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     2521 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2581 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2641 gggagggccc cggtgccgaa ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa
     2701 accctggtcc aatgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
     2761 tcaatgaagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     2821 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctggcaa
     2881 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     2941 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
     3001 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     3061 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     3121 tgggccactt tacactgggc tgcgtattgg aggatcagga gcatcaagta gcaaaagagg
     3181 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     3241 tcgaccatca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     3301 tggagaaaca gctaaagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     3361 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     3421 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat cgatccaaga
     3481 tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3601 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3661 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3721 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3781 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3841 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3901 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3961 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4021 gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta agaccaatga
     4081 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4141 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4201 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4261 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4321 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     4381 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4561 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4621 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4681 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctttcaaa
     4741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6661 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6721 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6781 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6841 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     6901 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     6961 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     7021 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     7081 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     7141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7261 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     7321 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     7381 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     7441 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     7501 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     7561 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     7621 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     7681 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     7741 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     7801 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7861 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7921 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7981 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     8041 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     8101 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     8161 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     8221 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     8281 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     8341 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     8401 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     8461 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     8521 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     8581 ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt catccacaat
     8641 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8701 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8761 gtttattaca gggacagcag agatccactt tggccgcgaa tcgatatgtc gagtttactc
     8821 cctatcagtg ataga
//
