LOCUS       sequence-349868-        8319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one dox-repressible expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Spinelli JB, Rosen PC, Sprenger HG, Puszynska AM, Mann JL, Roessler 
            JM, Cangelosi AL, Henne A, Condon KJ, Zhang T, Kunchok T, Lewis CA, 
            Chandel NS, Sabatini DM
  TITLE     Fumarate is a terminal electron acceptor in the mammalian electron 
            transport chain.
  JOURNAL   Science. 2021 Dec 3;374(6572):1227-1237. doi: 
            10.1126/science.abi7495. Epub 2021 Dec 2.
  PUBMED    34855504
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abi7495"; journalName: "Science"; date: "2021-12-3-
            3"; volume: "374"; issue: "6572"; pages: "1227-1237"
COMMENT     Sequence Label: pCW57.1 tTA SDHB
FEATURES             Location/Qualifiers
     source          1..8319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    339..456
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(669..713)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    898..1131
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1624..1749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1796..1976
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1977..2203)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2231..2251)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2231..2249)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2270..2286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2270..2286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2283..2305)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2294..2310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2318..2324,2325..2342,2343..2348))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2363..2384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2501..2518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2672..3260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2752..2771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3431..4222,4223..4291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4054..4073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4292..4363)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4363..4498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4538..4659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4549..4568)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4603..4622
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4731..4964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5169..5180
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5578..5598
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5638..5646,5647..5682,5683..5685,5686..5721,
                     5722..5724,5725..5760,5761..5766,5767..6384))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6385..6438)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6448..6840)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6850..7360)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6913..6931)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      8231..8240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(8290..8319,1..285))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(8293..8317)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gcttatatag gcctcccacc gtacacgcct acctcgacat acgttctcta tcactgatag
       61 ggagtaaact cgacatacgt tctctatcac tgatagggat aaactcgaca tacgttctct
      121 atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga
      181 catacgttct ctatcactga tagggagtaa actcgacatc gttctctatc actgataggg
      241 agtaaactcg acatacgttc tctatcactg atagggagta aactcgacat atcgattcgc
      301 ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
      361 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
      421 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
      481 ctcgaggtcg agaattgtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      541 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      601 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      661 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      721 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      781 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      841 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      901 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      961 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
     1021 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
     1081 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
     1141 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
     1201 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     1261 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     1321 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     1381 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     1441 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     1501 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     1561 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     1621 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     1681 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     1741 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     1801 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     1861 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     1921 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
     1981 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
     2041 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
     2101 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
     2161 gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct tgcagctcca
     2221 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2281 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2341 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2401 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2461 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4321 tttatcaggg ttattgtctc atgagcggat acatatttga aaggcctcca aaaaagcctc
     4381 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4441 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4501 gcggagttag gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga
     4561 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4621 gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca
     4681 tttctttgca agttataaat actgaataat aagatgacat gaactactac tgctagagat
     4741 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     4801 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     4861 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     4921 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     4981 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtacctctag
     5041 tccggacgcc gaggcgaaac aggcggggag gcggcccaaa gggagatccg actcgtctga
     5101 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     5161 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     5221 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
     5281 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     5341 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     5401 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     5461 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     5521 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     5581 agcgtaaaag gagcaacata gttaagaata cgatatcttg gatcgatcct tacttagtta
     5641 cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc
     5701 aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggc
     5761 cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc
     5821 gaaaaggaag gcaggttcgg ctccctgatg gtcgaacagc tcaattgctt gtctcagaag
     5881 tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg
     5941 atcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt
     6001 ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg
     6061 tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc
     6121 acagcggaat gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg
     6181 gcagaagtgg gtatgatgcc tgtccagcat ctcgattgcc agggcatcga gcagggcccg
     6241 cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt
     6301 ccttgtcgtc aggccttcga taccgacttc attgagtaat tccagagcag agtttatgac
     6361 tttgctcttg tccagtctag acattggacc agggttttct tcaacatcac cacaagtgag
     6421 gagagaacct ctaccttcgg caccggggcc ctcccacaca taaccagagg gcagcaattc
     6481 acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg
     6541 atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc
     6601 atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag
     6661 caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat
     6721 gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct
     6781 gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggaaccat
     6841 ggtgaattgc tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt
     6901 gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac
     6961 cgccccacac cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc
     7021 tgagcagccg ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg
     7081 ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc
     7141 cgcgaaccgc aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc
     7201 cacaagggta gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga
     7261 cccagggtcg gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc
     7321 aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc ggatccgaat tctcccgtcg
     7381 accctttaaa ctgaagcttt cttctcctta taggttgcca tcattttctt gatctctgca
     7441 atagctttcc ctggattcag acccttagga caggtccttg tgcagttcat gatggtgtgg
     7501 cagcggtata gagagaatgg gtcctgcagc ttggccaggc gctcctctgt gaagtcatct
     7561 ctggagtcaa tcatccagcg ataggcctgc ataagaactg caggccccag atatttgtct
     7621 ccgttccacc agtagctggg gcagctggtg ctacagcagg cacagagaat gcactcgtag
     7681 agcccgtcca gtttctcacg ctcttctatg gactgcagat actgctgctt gccttcctga
     7741 gattcatcct tcttcttcaa ataaggctca atggatttgt actgtgcata gaagttgctc
     7801 aaatcgggaa caagatcttt gattacgtac atgtgtggaa gagggtagat ttttgagacc
     7861 ttattgaggt tggtgtcaat ccttcgggtg caagctagag tgttgcctcc attgatgttc
     7921 attgcacaag agccacagat gccttctctg catgatcttc ggaaggtcaa agtagagtca
     7981 acttcattct taatcttgat taaagcatcc aataccatgg ggccacattt attaaggtca
     8041 acttcataag tctgcatatg aggtttgtct ccagccttgt ctgggtccca tcgatagatg
     8101 gcaaatttct tgatacgggg agctgtggct gcagctgtct gggctcctcg ggaggcctgc
     8161 aggcaggctc cgccaagggt tgtggccggc aaccggcgcc tcaaggagag ggcgaccacc
     8221 gccgccatgc ccatggtggc tggcggatcc ttaattaaag tctagaggat gatgctagcc
     8281 aattctccag gcgatctgac ggttcactaa acgagctct
//
