LOCUS       sequence-349869-        8792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one dox-inducible expression of Alternative Oxidase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Spinelli JB, Rosen PC, Sprenger HG, Puszynska AM, Mann JL, Roessler 
            JM, Cangelosi AL, Henne A, Condon KJ, Zhang T, Kunchok T, Lewis CA, 
            Chandel NS, Sabatini DM
  TITLE     Fumarate is a terminal electron acceptor in the mammalian electron 
            transport chain.
  JOURNAL   Science. 2021 Dec 3;374(6572):1227-1237. doi: 
            10.1126/science.abi7495. Epub 2021 Dec 2.
  PUBMED    34855504
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abi7495"; journalName: "Science"; date: "2021-12-3-
            3"; volume: "374"; issue: "6572"; pages: "1227-1237"
COMMENT     Sequence Label: pCW57.1_AOX-FLAG
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     222..242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        270..496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             497..677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    724..849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1342..1575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1760..1804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2017..2134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2188..2502
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2196..2214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2232..2250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2267..2285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2303..2321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2339..2357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2374..2392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2410..2428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2475..2499
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2523..2532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3672..3695
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3710..4220
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4139..4157
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4230..4826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4230..4249)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4726..4746
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4827..4880
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4881..5498,5499..5504,5505..5540,5541..5543,5544..5579,
                     5580..5582,5583..5618,5619..5627)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5667..5687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6085..6096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6301..6534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6606..6727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6643..6662)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6697..6716
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6767..6902
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6902..6973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6974..7042,7043..7834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7192..7211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8005..8593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8494..8513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8747..8764
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      241 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
      301 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      361 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      421 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
      481 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      661 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      721 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      781 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      841 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      901 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      961 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1021 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1081 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1141 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1201 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1261 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1321 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1381 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1441 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1501 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1561 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1621 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1681 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1741 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1801 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1861 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     1921 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacaatt
     1981 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2041 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2101 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2161 tccactttgg ccgcgaatcg atatgtcgag tttactccct atcagtgata gagaacgtat
     2221 gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat
     2281 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2341 cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt
     2401 cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag
     2461 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat tggctagcat
     2521 tcgccaccat gggtatgatt gggttgagca cgtaccgcaa tcttcctaca ctgctgacga
     2581 caacgaccgt catatctact accttgaggt caaagcaact cttgagattt acaacaacta
     2641 caagcactaa gtctggttct agtaccagta cggctgcaac gaccgtgggg aactcaaacc
     2701 ccaagtcacc tattgatgag gacaaccttg aaaaaccagg gactatccct acgaaacata
     2761 aaccgttcaa tattcagaca gaggtctata ataaggcggg cattgaggct aacgatgatg
     2821 ataagtttct gaccaaacca acctaccgac atgaagactt tacagaggct ggtgtgtacc
     2881 gggtccatgt tacacacaga ccaccccgga cgatcggtga caagattagc tgttatggaa
     2941 cactcttttt tagaaaatgc ttcgatctgg ttacaggcta tgccgtgcca gacccggata
     3001 aacccgatca atacaagggt accaggtggg agatgaccga aggtaaatgg atgacacgct
     3061 gcatctttct ggaatccatc gctggcgtgc cgggtagcgt agcaggattt gtgcggcatc
     3121 ttcactcact tcgcatgctc acacgcgaca aagcttggat cgaaactctg catgatgaag
     3181 cctacaacga gcgaatgcac ctcctgacat tcattaaaat tgggaagcct agctggttta
     3241 cccgctcaat aatttatatc ggtcagggtg tctttaccaa tatattcttc ttggtatact
     3301 tgatgaaccc ccgatactgt caccggttcg tcggctatct cgaagaggaa gccgttagga
     3361 cctataccca cctgattgac gagcttgacg acccgaacaa acttcccgac tttcaaaagc
     3421 tgcccattcc gaatatcgct gttcaatatt ggccggaatt gacacccgaa tccagtttca
     3481 aagacctgat cctccggata cgcgctgatg aagctaagca cagggaaatc aaccatacct
     3541 tcgcgaacct cgaacagtgg caagatcgca acccctttgc tctcaaaatc aaggactccg
     3601 acaaacccca gccaaactat aatctcgacg taactcgccc gcagggttgg gaacgcaagg
     3661 acctctacct tgattacaag gatgacgatg acaagtagac taaggatccg gggttggggt
     3721 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     3781 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     3841 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     3901 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3961 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     4021 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     4081 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     4141 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     4201 tcaccgacct ctctccccag caattcacca tgaccgagta caagcccacg gtgcgcctcg
     4261 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     4321 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     4381 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4441 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4501 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4561 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     4621 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4681 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4741 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4801 ggtgcatgac ccgcaagccc ggtgccgaag gtagaggttc tctcctcact tgtggtgatg
     4861 ttgaagaaaa ccctggtcca atgtctagac tggacaagag caaagtcata aacggagctc
     4921 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     4981 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     5041 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     5101 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     5161 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     5221 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     5281 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     5341 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     5401 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     5461 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     5521 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     5581 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc
     5641 gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6001 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6121 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6181 tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag gtacctttaa
     6241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6301 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6541 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6601 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6721 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6841 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6901 cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6961 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7021 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7081 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7141 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7201 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7261 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7321 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7381 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7441 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7501 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7561 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7621 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7681 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7741 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7801 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7861 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7921 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7981 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8041 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8101 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8161 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8221 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8281 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8341 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8401 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8461 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8521 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8581 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8641 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8701 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8761 cgaggaagcg gaagagcgcc caatacgcaa ac
//
