LOCUS       sequence-34987-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Koushik SV, Vogel SS
  TITLE     Energy migration alters the fluorescence lifetime of Cerulean: 
            implications for fluorescence lifetime imaging Forster resonance 
            energy transfer measurements.
  JOURNAL   J Biomed Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367.
  PUBMED    18601528
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomed 
            Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367."
COMMENT     Sequence Label: V-Kras
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(205..456)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(247..268)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     596..615
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacagttaca caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
      121 ggccgctcac ataattacac actttgtctt tgacttcttt ttcttctttt taccatcttt
      181 gctcatcttt tctttatgtt ttcgcttgta cagctcgtcc atgccgagag tgatcccggc
      241 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      301 cttggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      361 ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat
      421 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
      481 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      541 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      601 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      661 ggacttgaa
//
