LOCUS       sequence-349877-        6575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for neuronal expression of soma-targeted 
            (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence 
            attaching the ribosomal tag to the jGCaMP8m protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-RiboL1-jGCaMP8m
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     784..805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(911..932)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1172..1191)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2520..3108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2573..2593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2991..3002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3140..3616
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3339..3358)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3656..3796
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3656..3785
                     /label=AAV2 ITR
     rep_origin      3871..4326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3958..3977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4168..4189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4343..4362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4462..4484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4522..4540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4608..4712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4713..4781,4782..5573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4931..4950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5744..6332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6233..6252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6394..6523
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg ctcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagaggatc cacccgccac
      481 catgcatcat caccatcatc acacgcgtcg caagaagacc ttcaaggagg tggccaccgc
      541 cgtgaagatc atcggccgcc tgatgggcct gaagatcaac gtctatatca aggccgacaa
      601 gcagaagaac ggcatcaagg cgaacttcca catccgccac aacatcgagg acggcggcgt
      661 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      721 cgacaaccac tacctgagcg tggagtccaa actttcgaaa gaccccaacg agaagcgcga
      781 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      841 gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt tcaccggggt
      901 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      961 cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1021 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1081 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1141 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1201 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1261 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ctgccggacc aactgactga
     1321 agagcagatc gcagaataca aagaggcttt ctccctattt gacaaggacg gggatgggac
     1381 aataacaacc aaggagctgg ggacggtgat gcggtctctg gggcagaacc ccacagaagc
     1441 agagctgcag gacatgatca atgaagtaga tgccgacggt gacggcacaa tcgacttccc
     1501 tgagttcctg acaatgatgg caagaaaaat gaaatacagg gacacggaag aagaaattag
     1561 agaagcgttc ggtgtgtttg ataaggatgg caatggctac atcagtgcag cagagcttcg
     1621 ccacgtgatg acaaaccttg gagagaagtt aacagatgaa gaggttgatg aaatgatcag
     1681 ggaagcagac atcgatgggg atggtcaggt aaactacgaa gagtttgtac aaatgatgac
     1741 agcgaagggg ggcagcggtg ggtctggagg gactgggggt agcggtggta gcggtgggac
     1801 agggggaagc ggcggtagtg gtggaacaag cagcaaagtc tcacgcgaca ccctgtacga
     1861 ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc cgcaagtttc tggagacggt
     1921 ggagctgcag atcagcctga agaactacga ccctcagaag gacaaacgtt tctcgggcac
     1981 cgtcaggctc aagtccaccc cacgccccaa gttctcggtg tgcgttctgg gggaccagca
     2041 gcactgtgat gaagccaagg ccgtggatat cccccacatg gacatcgagg cgctcaagaa
     2101 acttaacaaa aacaagaagt tggtcaagaa gctggctaag aagtacgatg cctttttggc
     2161 ctctgagtct ctgattaagc agatcccacg tatcctgggc ccaggcctaa acaaggctgg
     2221 caagttcccc tccctgctga cacacaatga aaacatggtg gccaaagtgg atgaggtgaa
     2281 atcgacaatc aagttccaga tgaagaaggt gctgtgtttg gccgtcgctg ttggccacgt
     2341 gaagatgacc gatgatgagc tagtctacaa cattcatctg gctgtcaatt tcttggtgtc
     2401 cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac atcaagagca ccatgggcaa
     2461 gccccagcgt ctgtattagg gcgcgcccct gcagggaatt cgatatcaag cttatcgata
     2521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2881 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2941 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     3121 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     3181 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     3241 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3301 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3361 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3421 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3481 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3541 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3601 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     3661 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3721 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3781 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3841 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3901 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     3961 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4021 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4081 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4141 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4201 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4261 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4321 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4381 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4441 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4501 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4561 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4681 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4741 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4801 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4861 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4921 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4981 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5041 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5101 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5161 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5221 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5281 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5341 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5401 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5461 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5521 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5581 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5641 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6361 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
     6421 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     6481 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttta
     6541 attaagtgtc tagactgcag agggccctgc gtatg
//
