LOCUS       sequence-349899-        9468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one dox-repressible expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Spinelli JB, Rosen PC, Sprenger HG, Puszynska AM, Mann JL, Roessler 
            JM, Cangelosi AL, Henne A, Condon KJ, Zhang T, Kunchok T, Lewis CA, 
            Chandel NS, Sabatini DM
  TITLE     Fumarate is a terminal electron acceptor in the mammalian electron 
            transport chain.
  JOURNAL   Science. 2021 Dec 3;374(6572):1227-1237. doi: 
            10.1126/science.abi7495. Epub 2021 Dec 2.
  PUBMED    34855504
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abi7495"; journalName: "Science"; date: "2021-12-3-
            3"; volume: "374"; issue: "6572"; pages: "1227-1237"
COMMENT     Sequence Label: pCW57.1 tTA SDHA
FEATURES             Location/Qualifiers
     source          1..9468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     218..237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     471..488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    605..626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(641..646,647..664,665..671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    679..695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     684..706
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     703..719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     703..719
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     738..758
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        740..758
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        786..1012
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1013..1193
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1240..1365
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1858..2091
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2276..2320
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2533..2650
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2704..3018
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2748..2766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2783..2801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2890..2908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2926..2944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2991..3015
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3066..3075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5097..5607
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5526..5544
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5617..6009
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6019..6072
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6073..6690,6691..6696,6697..6732,6733..6735,6736..6771,
                     6772..6774,6775..6810,6811..6819)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6859..6879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7277..7288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7493..7726
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7798..7919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7835..7854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7889..7908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7959..8094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8094..8165
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8166..8234,8235..9026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8384..8403)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9197..9468,1..317)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
       61 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      481 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      541 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      601 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      661 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      721 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
      781 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
      841 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      901 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      961 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     1021 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     1081 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     1141 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1201 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     1261 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     1321 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     1381 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1441 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1501 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1561 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1621 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1681 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     1741 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1801 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1861 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1921 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1981 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2041 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2101 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     2161 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2221 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2281 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2341 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2401 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2461 gtttcagacc cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt
     2521 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2581 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2641 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat
     2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2761 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2821 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2881 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2941 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     3001 gtgaaccgtc agatcgcctg gagaattggc tagcatcatc ctctagactt taattaagga
     3061 tccgcgccac catgggaatg agtggtgtcc gaggactgtc aaggctgctt tctgcgagga
     3121 ggcttgcgtt ggcgaaggcc tggcctacgg tcctgcaaac cggaacccgc ggctttcatt
     3181 ttacggtgga cggcaacaaa agagccagtg ccaaagtcag cgacagtatc tctgcgcaat
     3241 accctgttgt agatcatgaa tttgacgccg tcgttgttgg agcaggaggt gctgggctcc
     3301 gggccgcctt cggtctctca gaagccgggt tcaacacagc gtgcgtcacc aagttgttcc
     3361 caactcgaag tcacactgtc gccgctcaag gcgggataaa tgctgccctg ggcaacatgg
     3421 aggaggataa ttggaggtgg catttttacg acactgtaaa ggggagtgac tggctgggag
     3481 accaagacgc aattcactac atgacagagc aggcccccgc cgccgtcgtg gagttggaga
     3541 actacggcat gccttttagt cggacggagg acgggaagat ataccaaagg gcctttggtg
     3601 gtcaatcttt gaaattcgga aaaggaggac aagcacatcg atgctgttgc gtagccgaca
     3661 ggacgggtca ttccttgctt cacacgctct acggtcggag tctccgctat gatacaagct
     3721 acttcgtgga atatttcgcc ctcgatctcc tcatggaaaa tggggaatgt cggggagtca
     3781 tcgccttgtg tatcgaggac ggttctatcc accgcattag agcgaagaat accgtcgtag
     3841 ctactggggg gtatgggcga acatacttca gctgcaccag cgcgcacact agcacagggg
     3901 acggaacggc tatgataacg cgagccgggc tgccatgcca agatttggag ttcgtacaat
     3961 tccatccaac cggaatatat ggagccggtt gcttgataac tgaaggctgt aggggcgagg
     4021 ggggcatttt gattaacagc caaggcgagc gcttcatgga acggtatgcg ccggtggcaa
     4081 aggatcttgc cagccgggat gttgtgtcac gcagcatgac tctggagatc cgagaaggtc
     4141 gcggttgcgg gcccgaaaag gaccacgtat acctgcagtt gcaccacctg ccccctgaac
     4201 aattggctac tcgcttgcca ggaatttctg agacggccat gatattcgct ggcgtggacg
     4261 tgactaagga gcctattccg gtgttgccca cggtacatta caatatgggg gggataccta
     4321 caaattataa agggcaggta cttcgccatg taaatggcca ggatcagatc gtgcccggcc
     4381 tttacgcgtg cggggaggca gcttgcgcgt ctgttcacgg ggcgaacaga ttgggggcca
     4441 atagcttgtt ggaccttgtg gtgttcggcc gcgcttgcgc attgagtatc gaggaatcct
     4501 gtcgccctgg tgacaaggtt cctcccatta agccaaatgc cggagaagaa agcgtgatga
     4561 acctcgacaa gctgagattc gcagacggca gcatacgcac atccgagttg cgcctcagca
     4621 tgcaaaaatc tatgcagaat cacgccgcgg tgtttagagt gggctccgtc ctgcaagagg
     4681 gctgtggaaa gatttccaaa ctctatggag acctgaaaca tttgaaaaca tttgaccgag
     4741 gaatggtctg gaataccgat ttggtggaaa ctctcgagct ccagaacctc atgctttgtg
     4801 ctttgcagac tatatacgga gccgaggctc gaaaagaaag tcgaggcgct cacgctaggg
     4861 aagactacaa ggtacgcatc gacgaatacg actatagtaa acctatacag gggcagcaaa
     4921 aaaagccttt tgaagagcac tggcggaaac ataccttgtc ttacgtcgat gtcggtacgg
     4981 gtaaagtgac gctggagtat cggcctgtga tagataaaac attgaacgaa gctgactgtg
     5041 ctacggtacc cccagccatc cggtcttact aagggtcgac gggagaattc ggatccgggg
     5101 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     5161 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     5221 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     5281 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     5341 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     5401 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     5461 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     5521 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     5581 gaccgaatca ccgacctctc tccccagcaa ttcaccatgg ttcctttgtc tcaagaagaa
     5641 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     5701 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     5761 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     5821 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     5881 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     5941 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     6001 tgggagggcc ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa
     6061 aaccctggtc caatgtctag actggacaag agcaaagtca taaactctgc tctggaatta
     6121 ctcaatgaag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     6181 gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctggca
     6241 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     6301 gactttctgc ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg
     6361 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     6421 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     6481 gtgggccact ttacactggg ctgcgtattg gaggatcagg agcatcaagt agcaaaagag
     6541 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     6601 ttcgaccatc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     6661 ctggagaaac agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac
     6721 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     6781 gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag
     6841 atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7201 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7381 ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg
     7441 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7501 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7561 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7621 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7681 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7741 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7921 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7981 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8041 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa
     8101 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8161 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8221 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8281 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8341 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8401 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8461 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8521 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8581 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8641 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8701 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8761 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8821 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8881 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8941 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9001 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9061 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9121 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9181 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9241 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9301 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9361 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9421 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggt
//
