LOCUS       sequence-34990-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Thaler C, Koushik SV, Puhl HL, Blank PS, Vogel SS
  TITLE     Structural rearrangement of CaMKIIalpha catalytic domains encodes 
            activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 14. 106(15):6369-74.
  PUBMED    19339497
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Apr 14. 106(15):6369-74."
COMMENT     Sequence Label: V32V
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(139..852,853..855))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(184..205)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     533..552
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagctgcata acagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
       61 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      121 tctagagtcg cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      181 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
      241 ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      301 gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt
      361 gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgtt
      421 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      481 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      541 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      601 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta
      661 gccgagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgag
      721 cttcagggtc agcttgccgt aagtggcatc gccctcgccc tcgccggaca cgctgaactt
      781 gtggccgttt acgtcgccgt ccagctcgac aaggatgggc accaccccgg tgaacagctc
      841 ctcgcccttg ctcaccacgg tggcgaccgg tcaaccgtaa cccccgggga attcccgcgg
      901 gccctggaa
//
