LOCUS       sequence-349908-       13710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SED1 biosensor for expression in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, 
            Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, 
            Covarrubias AA, Sukenik S, Dinneny JR
  TITLE     Intrinsically disordered protein biosensor tracks the 
            physical-chemical effects of osmotic stress on cells.
  JOURNAL   Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8.
  PUBMED    34521831
REFERENCE   2  (bases 1 to 13710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-14"; volume: "12(1):5438. doi"; pages: " 
            10.1038/s41467-021-25736-8"
COMMENT     Sequence Label: pGPTVII-nlsSED1
FEATURES             Location/Qualifiers
     source          1..13710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             473..1123
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(1311..1328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1562..1581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            1804..1913
                     /note="incP origin of transfer"
     CDS             1946..2317
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      2444..3155
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  3778..4545
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             4737..5531
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             5830..6978
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    8574..8598
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(8845..8866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9057..9076
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9201..9223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9215..9232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9216..9232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(9245..9445)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(9480..10031)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10082..10261)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             11318..11338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12856..12867
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    join(13694..13710,1..8)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
       61 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc ccagatctgg
      121 cgccggccag cgagacgagc aagattggcc gccgcccgaa acgatccgac agcgcgccca
      181 gcacaggtgc gcaggcaaat tgcaccaacg catacagcgc cagcagaatg ccatagtggg
      241 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
      301 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtatgttgg
      361 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
      421 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
      481 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
      541 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
      601 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
      661 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
      721 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
      781 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
      841 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
      901 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
      961 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     1021 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     1081 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     1141 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     1201 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     1261 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cttccgcttc ctcgctcact
     1321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct tttccgctgc
     1681 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1741 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1801 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1861 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc
     1921 cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca ggaagggcag
     1981 cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg aaaaggcggc
     2041 ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct acaaaatcac
     2101 gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg acctgggccg
     2161 cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc ggttcggtga
     2221 tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc ttggcaaggt
     2281 catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc taaaacggcc
     2341 ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag agcgacttcg
     2401 cggagctggt gaagtacatc accgacgagc aaggcaagac cgagcgcctt tccgacgctc
     2461 accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca
     2521 gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg
     2581 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
     2641 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
     2701 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
     2761 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
     2821 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
     2881 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
     2941 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
     3001 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
     3061 ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc
     3121 ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gcagtccttg ccattgccgg
     3181 gatcggggca gtaacgggat gggcgatcag cccgagcgcg acgcccggaa gcattgacgt
     3241 gccgcaggtg ctggcatcga cattcagcga ccaggtgccg ggcagtgagg gcggcggcct
     3301 gggtggcggc ctgcccttca cttcggccgt cggggcattc acggacttca tggcggggcc
     3361 ggcaattttt accttgggca ttcttggcat agtggtcgcg ggtgccgtgc tcgtgttcgg
     3421 gggtgcgata aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa
     3481 aacgagaatt ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa
     3541 gacgaagagg atgaagagga tgaggaggca gattgccttg aatatattga caatactgat
     3601 aagataatat atcttttata tagaagatat cgccgtatgt aaggatttca gggggcaagg
     3661 cataggcagc gcgcttatca atatatctat agaatgggca aagcataaaa acttgcatgg
     3721 actaatgctt gaaacccagg acaataacct tatagcttgt aaattctatc ataattgggt
     3781 aatgactcca acttattgat agtgttttat gttcagataa tgcccgatga ctttgtcatg
     3841 cagctccacc gattttgaga acgacagcga cttccgtccc agccgtgcca ggtgctgcct
     3901 cagattcagg ttatgccgct caattcgctg cgtatatcgc ttgctgatta cgtgcagctt
     3961 tcccttcagg cgggattcat acagcggcca gccatccgtc atccatatca ccacgtcaaa
     4021 gggtgacagc aggctcataa gacgccccag cgtcgccata gtgcgttcac cgaatacgtg
     4081 cgcaacaacc gtcttccgga gactgtcata cgcgtaaaac agccagcgct ggcgcgattt
     4141 agccccgaca tagccccact gttcgtccat ttccgcgcag acgatgacgt cactgcccgg
     4201 ctgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa aatcgtgttg
     4261 aggccaacgc ccataatgcg ggctgttgcc cggcatccaa cgccattcat ggccatatca
     4321 atgattttct ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt
     4381 ttacggcagt gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc
     4441 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
     4501 caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccataa ttgtggtttc
     4561 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     4621 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     4681 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     4741 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     4801 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     4861 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     4921 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     4981 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     5041 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     5101 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     5161 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     5221 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     5281 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     5341 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     5401 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     5461 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     5521 aattgtttta gtacctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac
     5581 ttcttccgca tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag
     5641 gggtcgctgg tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag
     5701 acggtctacg ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca
     5761 ggcgggtcaa atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa
     5821 ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg
     5881 gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc
     5941 gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac
     6001 agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt
     6061 cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact
     6121 atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc
     6181 aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg
     6241 ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct
     6301 gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt
     6361 ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat
     6421 gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg
     6481 atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat
     6541 tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc
     6601 gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt
     6661 ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct
     6721 ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga
     6781 cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc
     6841 cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa
     6901 gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg
     6961 gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagccagc
     7021 gctttactgg catttcagga acaagcgggc actgctcgac gcacttgctt cgctcagtat
     7081 cgctcgggac gcacggcgcg ctctacgaac tgccgataaa cagaggatta aaattgacaa
     7141 ttgtgattaa ggctcagatt cgacggcttg gagcggccga cgtgcaggat ttccgcgaga
     7201 tccgattgtc ggccctgaag aaagctccag agatgttcgg gtccgtttac gagcacgagg
     7261 agaaaaagcc catggaggcg ttcgctgaac ggttgcgaga tgccgtggca ttcggcgcct
     7321 acatcgacgg cgagatcatt gggctgtcgg tcttcaaaca ggaggacggc cccaaggacg
     7381 ctcacaaggc gcatctgtcc ggcgttttcg tggagcccga acagcgaggc cgaggggtcg
     7441 ccggtatgct gctgcgggcg ttgccggcgg gtttattgct cgtgatgatc gtccgacaga
     7501 ttccaacggg aatctggtgg atgcgcatct tcatcctcgg cgcacttaat atttcgctat
     7561 tctggagctt gttgtttatt tcggtctacc gcctgccggg cggggtcgcg gcgacggtag
     7621 gcgctgtgca gccgctgatg gtcgtgttca tctctgccgc tctgctaggt agcccgatac
     7681 gattgatggc ggtcctgggg gctatttgcg gaactgcggg cgtggcgctg ttggtgttga
     7741 caccaaacgc agcgctagat cctgtcggcg tcgcagcggg cctggcgggg gcggtttcca
     7801 tggcgttcgg aaccgtgctg acccgcaagt ggcaacctcc cgtgcctctg ctcaccttta
     7861 ccgcctggca actggcggcc ggaggacttc tgctcgttcc agtagcttta gtgtttgatc
     7921 cgccaatccc gatgcctaca ggaaccaatg ttctcggcct ggcgtggctc ggcctgatcg
     7981 gagcgggttt aacctacttc ctttggttcc gggggatctc gcgactcgaa cctacagttg
     8041 tttccttact gggctttctc agccccagat ctggggtcga tcagccgggg atgcatcagg
     8101 ccgacagtcg gaacttcggg tccccgacct gtaccattcg gtgagcaatg gataggggag
     8161 ttgatatcgt caacgttcac ttctaaagaa atagcgccac tcagcttcct cagcggcttt
     8221 atccagcgat ttcctattat gtcggcatag ttctcaagat cgacagcctg tcacggttaa
     8281 gcgagaaatg aataagaagg ctgataattc ggatctctgc gagggagatg atatttgatc
     8341 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
     8401 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
     8461 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
     8521 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
     8581 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
     8641 ttaatgtact ggggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     8701 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     8761 aaaatcctgt ttgatggtgg ttccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8821 cccgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8881 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8941 tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     9001 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     9061 aagaaagcga aaggagcggg cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     9121 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9181 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat
     9241 tcccatcttg aaagaaatat agtttaaata tttattgata aaataacaag tcaggtatta
     9301 tagtccaagc aaaaacataa atttattgat gcaagtttaa attcagaaat atttcaataa
     9361 ctgattatat cagctggtac attgccgtag atgaaagact gagtgcgata ttatgtgtaa
     9421 tacataaatt gatgatatag ctagcttagc tcatcggggg atcgatcctc taggggtcat
     9481 cagatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc
     9541 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
     9601 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
     9661 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
     9721 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag
     9781 tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc
     9841 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
     9901 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
     9961 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
    10021 tctgggctca tggtagatcc ccctagagtc gagatctgga ttgagagtga atatgagact
    10081 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
    10141 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
    10201 gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt
    10261 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac
    10321 tcccttaatt ctccgctcat gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
    10381 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
    10441 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
    10501 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
    10561 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc
    10621 tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga agcttcgacg agtcagtaat
    10681 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac
    10741 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac
    10801 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg
    10861 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagacctc
    10921 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc
    10981 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt
    11041 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga
    11101 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag
    11161 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg
    11221 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct
    11281 gagtttttct gattaacaga ctagtatgat gctgcagcct aagaagaaga gaaaggttgg
    11341 aggggtgagc aaaggtgaag aactgtttac cggtgttgtt ccgattctgg ttgaactgga
    11401 tggtgacgtt aatggtcaca aattttcagt tagcggtgaa ggcgaaggtg atgcaaccta
    11461 tggtaaactg accctgaaat ttatctgtac caccggcaaa ctgccggttc cgtggccgac
    11521 cctggttacc accctgagct ggggtgttca gtgttttgca cgttatccgg atcacatgaa
    11581 acagcacgat tttttcaaaa gcgcaatgcc ggaaggttat gttcaagaac gtaccatctt
    11641 cttcaaagat gacggcaact ataaaacccg tgccgaagtt aaatttgaag gtgataccct
    11701 ggtgaatcgc attgaactga aaggcatcga ttttaaagag gatggtaata tcctgggcca
    11761 caaactggaa tataatgcca ttcatggcaa cgtgtatatc accgcagata aacagaaaaa
    11821 cggcatcaaa gcaaattttg gcctgaactg caatattgaa gatggttcag ttcagctggc
    11881 agatcattat cagcagaata ccccgattgg tgatggtccg gttctgctgc cggataatca
    11941 ttatctgagc acccagagca aactgagcaa agatccgaat gaaaaacgtg atcacatggt
    12001 gctgctggaa tttgttaccg cagcaggtat taccctgggt atggatgaac tgtataaaga
    12061 gctcatgcag tcgatgaaag aaacagcttc gaatattgca gcttctgcaa aatctggcat
    12121 ggacaaaacc aaagctacct tggaggaaaa ggcggagaag atgaagacac gagaccctgt
    12181 tcagaaacag atggctacac aggttaaaga agataagatc aatcaagctg agatgcagaa
    12241 gagagaaacg cgtcagcaca acgcggccat gaaagaagcg gctggagccg gaaccggttt
    12301 aggtttgggg acggccactc actcgaccac tggacaagtc ggacacggca ctgggacaca
    12361 tcagatgtcg gctctgcctg gtcacggaac gggacaactg accgaccgcg ttgtggaggg
    12421 cacggctgtg accgacccga ttggaaggaa cactggaact ggtcggacaa ccgctcataa
    12481 cacacacgtt ggtggtggtg gtgccaccgg gtacggaacc ggcgggggat atactggaag
    12541 atctatggtg tcaaaaggtg aggaactgtt tacaggcgtg gtgccgatcc tggtagagct
    12601 ggacggggat gtgaatggcc ataaattcag cgtttcaggt gaaggtgagg gcgacgccac
    12661 gtacggaaaa ctgacactga aattcatttg cacaacaggt aaactgcctg tgccttggcc
    12721 tacactggtg accacctttg gttatggtct gatgtgcttt gctcgctatc ctgaccacat
    12781 gaaacaacat gatttcttta aatctgccat gcctgaaggc tacgtgcaag agcgcaccat
    12841 ttttttcaaa gacgatggga attacaaaac acgtgcggag gtgaaattcg agggcgatac
    12901 actggttaac cgtatcgagc tgaaaggtat cgacttcaaa gaggacggaa acattctggg
    12961 tcataaactg gaatacaact acaacagcca taacgtgtac atcatggccg acaaacaaaa
    13021 aaacgggatt aaagtgaact tcaaaatccg ccacaacatc gaagatggca gcgtgcagct
    13081 ggccgaccac tatcaacaaa acacaccgat cggcgacggt cctgtactgc tgcctgacaa
    13141 ccactatctg tcatatcaga gcgcactgtc aaaagatcct aacgagaaac gcgaccacat
    13201 ggttctgctg gaattcgtga cagccgctgg cattacactg ggcatggacg agctgtacaa
    13261 ataacccggg taggagctca tatgaagatg aagatgaaat atttggtgtg tcaaataaat
    13321 agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt
    13381 tttctaatat taaatgaatg taagatctca ttataatgaa taaacaaatg tttctataat
    13441 ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt
    13501 atggactatg gaatatgatt aaagataagg aattcagatc ggctgagtgg ctccttcaac
    13561 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
    13621 taacgtgact cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagttta
    13681 aactatcagt gtttgacagg atatattggc
//
