LOCUS       sequence-34992-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Thaler C, Koushik SV, Puhl HL, Blank PS, Vogel SS
  TITLE     Structural rearrangement of CaMKIIalpha catalytic domains encodes 
            activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 14. 106(15):6369-74.
  PUBMED    19339497
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Apr 14. 106(15):6369-74."
COMMENT     Sequence Label: VVVVVV
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(150..860,861..863))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(192..213)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     541..560
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     800..821
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attaagctgc ataacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
       61 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      121 atgatcagtt atctagatcc ggtggatccc ttgtacagct cgtccatgcc gagagtgatc
      181 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      241 ctcagcttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      301 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg
      361 cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
      421 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      481 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      541 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      601 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc
      661 aggccgtagc cgagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
      721 cagatgagct tcagggtcag cttgccgtaa gtggcatcgc cctcgccctc gccggacacg
      781 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
      841 aacagctcct cgcccttgct cac
//
