LOCUS       sequence-349964-       10329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL016:CMV::eGFP-iPAK4
FEATURES             Location/Qualifiers
     source          1..10329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3206..3919
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVILE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3236..3264)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     misc_feature    5021..5609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5074..5094)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5492..5503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5612..5628)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5613..5629)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6134..6314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6340..6357)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6346..6570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6616..7044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6703..6722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6913..6934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7053..7073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7058..7387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7238..7373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7300..7319
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7435..7482
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7501..7875
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8005..8126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8042..8061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8096..8115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8175..8191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8175..8191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8188..8210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8199..8215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8223..8229,8230..8247,8248..8253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8268..8289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8406..8423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8577..9165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8657..8676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9336..10127,10128..10196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9959..9978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10197..10301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatgag taaaggagaa gaacttttca ctggagttgt
     3241 cccaattctt gttgaattag atggtgatgt taatgggtac aaattttctg tcagtggaga
     3301 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     3361 actacctgtt ccatggccaa cacttgtcac tactctcact tatggtgttc aatgcttttc
     3421 aagatatcca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata
     3481 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt
     3541 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga
     3601 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat
     3661 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga
     3721 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatgaccc
     3781 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     3841 cgaaaagaga gaccacatgg tcattcttga gtttgtaacg gctgctggga ttacacatgg
     3901 catggatgaa ctatacaaat caggtggatc cgaagcagag gactggacag cagccctact
     3961 gaacaggggt cgcagtcgcc agcccctggt actaggggac aattgctttg ctgacttggt
     4021 gcacaactgg atggagctgc ctgaggaatt ccccgccgcc cctgctgttc ctgggccccc
     4081 tggcccccgc tcaccacagc gggagccaca gcgagtatcc catgagcagt tccgggctgc
     4141 cctgcagctg gtggtggacc caggcgaccc ccgctcctac ctggacaact tcatcaagat
     4201 tggcgagggc tccacgggca tcgtgtgcat cgccaccgtg cgcagctcgg gcaagctggt
     4261 ggccgtcaag aagatggacc tgcgcaagca gcagaggcgc gagctgctct tcaacgaggt
     4321 ggtaatcatg agggactacc agcacgagaa tgtggtggag atgtacaaca gctacctggt
     4381 gggggacgag ctctgggtgg tcatggagtt cctggaagga ggcgccctca ccgacatcgt
     4441 cacccacacc aggatgaacg aggagcagat cgcagccgtg tgccttgcag tgctgcaggc
     4501 cctgtcggtg ctccacgccc agggcgtcat ccaccgggac atcaagagcg actcgatcct
     4561 gctgacccat gatggcaggg tgaagctgtc agactttggg ttctgcgccc aggtgagcaa
     4621 ggaagtgccc cgaaggaagt cgctggtcgg cacgccctac tggatggccc cagagctcat
     4681 ctcccgcctt ccctacgggc cagaggtaga catctggtcg ctggggataa tggtgattga
     4741 gatggtggac ggagagcccc cctacttcaa cgagccaccc ctcaaagcca tgaagatgat
     4801 tcgggacaac ctgccacccc gactgaagaa cctgcacaag gtgtcgccat ccctgaaggg
     4861 cttcctggac cgcctgctgg tgcgagaccc tgcccagcgg gccacggcag ccgagctgct
     4921 gaagcaccca ttcctggcca aggcagggcc gcctgccagc atcgtgcccc tcatgcgcca
     4981 gaaccgcacc agatgagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     5041 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5101 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5161 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5221 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5281 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5341 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5401 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5461 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5521 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5581 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     5641 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     5701 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     5761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5821 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     5881 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     5941 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     6001 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6061 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     6121 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6181 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6241 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6301 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6361 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6421 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6481 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6541 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6601 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6781 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6841 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6901 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6961 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7021 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7081 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7141 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7201 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7261 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7321 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7381 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7441 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7501 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     7561 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     7621 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7681 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7741 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     7801 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     7861 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7921 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7981 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8041 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8101 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8161 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8221 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8341 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8401 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8761 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9001 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10321 ccacctgac
//
