LOCUS       sequence-349965-       11592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL034:cFos::HaloTag-iPAK4
FEATURES             Location/Qualifiers
     source          1..11592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3303..3314)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    6284..6872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6337..6357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6755..6766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6875..6891)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6876..6892)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7397..7577
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7603..7620)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7609..7833
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7879..8307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7966..7985)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8176..8197
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8316..8336)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8321..8650
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8501..8636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8563..8582
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8698..8745
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8764..9138
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9268..9389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9305..9324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9359..9378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9438..9454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9438..9454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9451..9473)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9462..9478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9486..9492,9493..9510,9511..9516))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9531..9552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9669..9686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9840..10428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9920..9939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10599..11390,11391..11459))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11222..11241
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11460..11564)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aaagctttcc tttaggaaca gaggcttcga gcctttaagg
     2641 ctgcgtactt gcttctccta ataccagaga ctcaaaaaaa aaaaaaaaag ttccagattg
     2701 ctggacaatg acccgggtct catcccttga ccctgggaac cgggtccaca ttgaatcagg
     2761 tgcgaatgtt cgctcgcctt ctctgccttt cccgcctccc ctcccccggc cgcggccccg
     2821 gttccccccc tgcgctgcac cctcagagtt ggctgcagcc ggcgagctgt tcccgtcaat
     2881 ccctccctcc tttacacagg atgtccatat taggacatct gcgtcagcag gtttccacgg
     2941 ccggtccctg ttgttctggg ggggggacca tctccgaaat cctacacgcg gaaggtctag
     3001 gagaccccct aagatcccaa atgtgaacac tcataggtga aagatgtatg ccaagacggg
     3061 ggttgaaagc ctggggcgta gagttgacga cagagcgccc gcagagggcc ttggggcgcg
     3121 cttccccccc cttccagttc cgcccagtga cgtaggaagt ccatccattc acagcgcttc
     3181 tataaaggcg ccagctgagg cgcctactac tccaaccgcg actgcagcga gcaactgaga
     3241 agactggata gagccggcgg ttccgcgaac gagcagtgac cgcgctccca cccagctctg
     3301 ctctgcagct cccaccagtg tctacccctg gaccccttgc cgggctttcc ccaaacttcg
     3361 accatgatgt tctcgggttt caacgccgac tacgaggcgt catcctcccg ctgcagtagc
     3421 gcctccccgg ccggggacag cctttcctac taccattccc cagccgactc cttctccagc
     3481 atgggctctc ctgtcaacac acaggtgagt ttggctttgt gtagccgcca ggtccgcgct
     3541 gagggtcgcc gtggaggaga cactggggtg tgactcgcag gggcgggggg gtcttccttt
     3601 ttcgctctgg agggagactg gcgcggtcag agcagcctta gcctgggaac ccaggacttg
     3661 tctgagcgcg tgcacacttg tcatagtaag acttagtgac cccttcccgc gcggcaggtt
     3721 tattctgagt ggcctgcctg cattcttctc tcggccgact tgtttctgag atcagccggg
     3781 gccaacaagt ctcgagcaaa gagtcgctaa ctagagtttg ggaggcggca aaccgcggca
     3841 atcccccctc ccggggcagc ctggagcagg gaggagggag gagggaggag ggtgctgcgg
     3901 gcgggtgtgt aaggcagttt cattgataaa aagcgagttc attctggaga ctccggagca
     3961 gcgcctgcgt cagcgcagac gtcagggata tttataacaa accccctttc gagcgagtga
     4021 tgccgaaggg ataacgggaa cgcagcagta ggatggaggg gaaaggctgc gctgcggaat
     4081 tcaagggagg atattgggag agcttttatc tccgatgagg tgcatacagg aagacataag
     4141 cagtctctga ccggaatgct tctctctccc tgcttcatgc gacactaggg ccacttgctc
     4201 cacctgtgtc tggaacctcc tcgctcacct ccgctttcct ctttttgttt tgtttcagga
     4261 cttttgcgca gatctctccg gatccatgat cggtactggc tttccattcg acccccatta
     4321 tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc
     4381 tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca
     4441 tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa
     4501 accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc
     4561 cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca
     4621 ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc
     4681 tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac
     4741 caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc
     4801 gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct
     4861 gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga
     4921 gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt
     4981 cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg
     5041 cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct
     5101 gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt cgacgctcga
     5161 gatttccggc gagccaacca cttcaggtgg atccgaagca gaggactgga cagcagccct
     5221 actgaacagg ggtcgcagtc gccagcccct ggtactaggg gacaattgct ttgctgactt
     5281 ggtgcacaac tggatggagc tgcctgagga attccccgcc gcccctgctg ttcctgggcc
     5341 ccctggcccc cgctcaccac agcgggagcc acagcgagta tcccatgagc agttccgggc
     5401 tgccctgcag ctggtggtgg acccaggcga cccccgctcc tacctggaca acttcatcaa
     5461 gattggcgag ggctccacgg gcatcgtgtg catcgccacc gtgcgcagct cgggcaagct
     5521 ggtggccgtc aagaagatgg acctgcgcaa gcagcagagg cgcgagctgc tcttcaacga
     5581 ggtggtaatc atgagggact accagcacga gaatgtggtg gagatgtaca acagctacct
     5641 ggtgggggac gagctctggg tggtcatgga gttcctggaa ggaggcgccc tcaccgacat
     5701 cgtcacccac accaggatga acgaggagca gatcgcagcc gtgtgccttg cagtgctgca
     5761 ggccctgtcg gtgctccacg cccagggcgt catccaccgg gacatcaaga gcgactcgat
     5821 cctgctgacc catgatggca gggtgaagct gtcagacttt gggttctgcg cccaggtgag
     5881 caaggaagtg ccccgaagga agtcgctggt cggcacgccc tactggatgg ccccagagct
     5941 catctcccgc cttccctacg ggccagaggt agacatctgg tcgctgggga taatggtgat
     6001 tgagatggtg gacggagagc ccccctactt caacgagcca cccctcaaag ccatgaagat
     6061 gattcgggac aacctgccac cccgactgaa gaacctgcac aaggtgtcgc catccctgaa
     6121 gggcttcctg gaccgcctgc tggtgcgaga ccctgcccag cgggccacgg cagccgagct
     6181 gctgaagcac ccattcctgg ccaaggcagg gccgcctgcc agcatcgtgc ccctcatgcg
     6241 ccagaaccgc accagatgag aattcgatat caagcttatc gataatcaac ctctggatta
     6301 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6361 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6421 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6481 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6541 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6601 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6661 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6721 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6781 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6841 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     6901 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6961 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7021 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7081 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7141 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     7201 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     7261 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7321 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     7381 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7561 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     7621 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7681 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7741 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7801 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7861 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7921 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7981 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8041 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8101 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8161 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8221 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8281 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8401 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8461 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8521 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8581 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8641 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     8701 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     8761 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     8821 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     8881 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     8941 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     9001 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     9061 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     9121 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     9181 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9241 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     9301 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9361 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     9421 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9481 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9541 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9601 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9901 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10261 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10321 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10501 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10561 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10621 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10681 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10741 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10801 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10861 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10921 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10981 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11041 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11101 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11161 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11221 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11281 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11341 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11401 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11581 gtgccacctg ac
//
