LOCUS       sequence-34998-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Thaler C, Koushik SV, Puhl HL, Blank PS, Vogel SS
  TITLE     Structural rearrangement of CaMKIIalpha catalytic domains encodes 
            activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 14. 106(15):6369-74.
  PUBMED    19339497
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Apr 14. 106(15):6369-74."
COMMENT     Sequence Label: V5V
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(182..433)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(224..245)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     573..592
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attatagctg cataacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
       61 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
      121 tatgatcagt tatctagatc cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt
      181 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      241 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtag ctcaggtagt
      301 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      361 cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
      421 tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      481 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      541 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      601 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      661 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gccgagggtg gtcacga
//
