LOCUS       sequence-35000-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Thaler C, Koushik SV, Puhl HL, Blank PS, Vogel SS
  TITLE     Structural rearrangement of CaMKIIalpha catalytic domains encodes 
            activation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 14. 106(15):6369-74.
  PUBMED    19339497
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Apr 14. 106(15):6369-74."
COMMENT     Sequence Label: VVVV
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(144..854,855..857))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(186..207)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     535..554
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     794..815
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actgcataac agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 agttatctag atccggtgga tcccttgtac agctcgtcca tgccgagagt gatcccggcg
      181 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
      241 ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      301 gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      361 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      421 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      481 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      541 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      601 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg
      661 tagccgaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      721 agcttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      781 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      841 tcctcgccct tgctcacc
//
