LOCUS       sequence-350001-       17555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying 
            PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction 
            site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for 
            PaldA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17555)
  AUTHORS   Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, 
            Karton A, Piggott AM, Chooi YH
  TITLE     Heterologous biosynthesis of elsinochrome A sheds light on the 
            formation of the photosensitive perylenequinone system.
  JOURNAL   Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. 
            eCollection 2019 Feb 7.
  PUBMED    30809363
REFERENCE   2  (bases 1 to 17555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c8sc02870b"; journalName: "Chem Sci"; date: "2018-11-22- 
            22"; volume: "10"; issue: "5"; pages: "1457-1465"
COMMENT     Sequence Label: pYFAC-CH4
FEATURES             Location/Qualifiers
     source          1..17555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3252..3320,3321..4112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3470..3489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4283..4871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4772..4791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5025..5042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5159..5180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5195..5200,5201..5218,5219..5225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5233..5249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5238..5260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5257..5273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5257..5273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      5290..6632
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6253..6300)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6673..6691
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6729..6751)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6851..6870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6894..7109
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7110..7913
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8195..8212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8195..8211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8204..8226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8511..8530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8552..13800
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     13970..13991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccccctggt atcatggttg ttgggtctca tcactcctac tcaacattgc acctgatagg
       61 gacatcagat gctggattac taaggtaatg taaggtcagt tcgagaccat ctgatattac
      121 cacaaataca atggcgagag agtttttcgt aaaagccaat ccttggcgtt tccagctgtt
      181 cctgacggtt gtaggcccaa gtccgcggga aaccgcccac aaagcggcgt ttttgcagat
      241 tggcagattt atgctggaaa cttactgggg agatggaggg gcacaagcgc tgtgattggt
      301 tttcaaagcc gcggccggat ggaacgaaga cataattcgg cggggacatg aaaatgtggg
      361 tgatcgatac ggaatttttg gttcttcgga ggcgacaaag ggcgcaacgg tcgaggttag
      421 tagttatctt gactcacact tacagggccc gtcttcggtc ttcttaagaa ctgggttttg
      481 ctgggacttc ccccccacct ctcttttcta ctgtgtctcg tatctatttc tatactcatt
      541 ctttcacttc tcttagtacc accattccct tctaaataca cagaatggct tccaacggta
      601 ccaatggcgc ctccgcctcc aacagcttca ctgtgaaggc cggcttggct cagatgctga
      661 agggtggtgt gattatggac gtcgtcaacg cggagcaggt atgagcgatt gtcatcagga
      721 tacttccagc cctttgacgc taacatgact tctacaacag gcccgcattg cggaggaggc
      781 cggtgccgct gccgtgatgg ccctggagag agtccccgcc gacatcagag cccagggtgg
      841 cgttgcccgc atgtctgacc ccagcatgat caaggagatc atggctgctg ttaccattcc
      901 tgtcatggcc aaagctcgta tcggacactt cgttgagtgc caggtaaggc tgcctttctc
      961 ccgtggaaag cctgcattgc agctaacatg tgtaattgtt agatcctcga agccattggc
     1021 gttgactaca tcgacgagtc cgaagtcctt acccctgccg atgatgtcta ccacgtgaag
     1081 aagcacgact acaaggttcc tttcgtctgt ggttgccgca acctgggcga ggcccttcgt
     1141 cggatcgccg agggtgccgc tatgatccgt accaagggtg aggccggtac cggagatgtt
     1201 gttgaagccg tcaagcacat gcgcacggtc aactcccaga tcgcccgcgc ccgctccatc
     1261 ctccagaatt ccaccgaccc cgagattgag ctgcgtgcct acgctcgtga gcttgaggtc
     1321 ccttatgagc ttctgcgcga gaccgccgag aagggccgtc ttcccgttgt caacttcgcc
     1381 gccggcggtg ttgccactcc cgctgatgcc gcactcatga tgcagctggg ctgcgacgga
     1441 gtgttcgtcg gctctggtat tttcaagtct ggtgatgcga agaagcgcgc caaggctatt
     1501 gtccaggccg tgactcacta caaggacccc aaggtcctcg ctgaagtcag cgagggtctg
     1561 ggtgaggcca tggttggtat caatgtctct cagatgcccg aggccgaccg attggccaag
     1621 agaggatggt aattgcacta ctatctctac ttgtgattct tcttatgttc ttgtcatgat
     1681 atgggcgttg gaaaagttga tatagcgttc tttgatgcat tttgcattca agactttcag
     1741 gttcattctt gttagggtgt tctgtgcatt tgtccttcat tatgtagaca ctcgcaggat
     1801 tgaagaagaa ctactctatt caatgacgat ctcttccatg aagaccaaaa actaaaaatt
     1861 ggtcatgtca acgtccagtg cacacgacat tttacccttc cccactaaca cttgttttgg
     1921 ctgtcgatca tgtggatgct gctactggtg gtgctaatct gcttgaagac gcggcgagta
     1981 gtatcaccaa gtaatatact tatattgcga agataattta tatctgatat ttcaattaca
     2041 aattctgaga ccactaccca gttgacggca ctcatgctgg gtgccttaga tagacgtcaa
     2101 ggtacacatc ctacggagtt acgatcggcg acttcaaaag aatgtcacac cggtaagata
     2161 cacacgttta tttcaaacgc cgaacttgaa tacacaccct gacagcgcca taagacaatt
     2221 tcctcttgga gcaaaagtgt agtgccagta cgagtgttgt ggaggaaggc tgcatacatt
     2281 gtgcctgtca ttaaacgatg agctcgtccg tattggcccc tgtaatgcca tgttttccgc
     2341 ccccaatcgt caaggttttc cctttgttag attcctacca gtcatctagc aagtgaggta
     2401 agctttgcca gaaacgccaa ggctttatct atgtagtcga taagcaaagt ggactgatag
     2461 cttaatatgg aaggtccctc aggacaagtc gacctgtgca gaagagataa cagcttggca
     2521 tcacgcatca gtgcctcctc tcagacagaa taagtcgcca taagttatcg accgaaccta
     2581 ggtagggtat atttggtgac gacaatacct cccgacgata cctggattag acaccgtatg
     2641 ccgcgaatta acgccttccg accaagtccc ttttggtgat cgacacttta ggatcatctc
     2701 ctgccagtcg cccagactga cggtgttatg gtggcgcgct atggctttcc tagccggtca
     2761 agctccgtgc actagagagc ggagaaggag ggggagttga tagggaatga tatctaggta
     2821 gagaagtact ccaaccagag atatgcggat caatgctccg gcagttgtta gcatacccca
     2881 acctcgttta agtatcctct tcttccatcc atctttatca ttatttgtct atgtgcatcc
     2941 ctagatcacg tcaattattt cgataaatgg atacgtgcac tcgggaatgc gtgattggct
     3001 cgtctcgtgt tctcagttcc tcattccccc ccacgttaca ttgacaacgg agtattagcc
     3061 tatggtcctg ttcgataagc ttgctccatc gcctccacta cgtcaagcgg agtatcagac
     3121 atgagctcta ccccagatat cgcctgctta tcgctggttg ctttgtcatg caagtacaga
     3181 tcacatcggc aattctgata acagatggtg ccattgtccc gtaatcaatt gcccattgaa
     3241 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3361 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3421 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3541 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3661 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3781 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3841 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3961 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4081 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4921 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4981 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5041 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5101 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5161 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5221 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5281 acgccactga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     5341 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     5401 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     5461 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     5521 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     5581 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     5641 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     5701 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     5761 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     5821 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     5881 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     5941 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     6001 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     6061 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     6121 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     6181 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     6241 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     6301 aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
     6361 actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
     6421 ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa
     6481 aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct
     6541 tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc
     6601 ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca
     6661 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     6721 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6781 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     6841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accataccac cttttcaatt
     6901 catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg
     6961 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     7021 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     7081 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     7141 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     7201 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     7261 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     7321 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     7381 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     7441 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     7501 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     7561 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     7621 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     7681 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     7741 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     7801 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     7861 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     7921 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     7981 agttattacc ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     8041 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     8101 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     8161 agatagggtt gagtggggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
     8221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8341 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8401 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8461 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     8521 tttcttccct tcctttctcg ccacgttcgc cagcttattt tttgtatact gttttgtgat
     8581 agcacgaagt ttttccacgg tatcttgtta aaaatatata tttgtggcgg gcttacctac
     8641 atcaaattaa taagagacta attataaact aaacacacaa gcaagctact ttagggtaaa
     8701 agtttataaa tgcttttgac gtataaacgt tgcttgtatt tattattaca attaaaggtg
     8761 gatagaaaac ctagagacta gttagaaact aatctcaggt ttgcgttaaa ctaaatcaga
     8821 gcccgagagg ttaacagaac ctagaagggg actagatatc cgggtaggga aacaaaaaaa
     8881 aaaaacaaga cagccacata ttagggagac tagttagaag ctagttccag gactaggaaa
     8941 ataaaagaca atgataccac agtctagttg acaactagat agattctaga ttgaggccaa
     9001 agtctctgag atccaggtta gttgcaacta atactagtta gtatctagtc tcctataact
     9061 ctgaagctag aataacttac tactattatc ctcaccactg ttcagctgcg caaacggagt
     9121 gattgcaagg tgttcagaga ctagttattg actagtcagt gactagcaat aactaacaag
     9181 gtattaacct accatgtctg ccatcaccct gcacttcctc gggctcagca gccttttcct
     9241 cctcattttc atgctcattt tccttgttta agactgtgac tagtcaaaga ctagtccaga
     9301 accacaaagg agaaatgtct taccactttc ttcattgctt gtctcttttg cattatccat
     9361 gtctgcaact agttagagtc tagttagtga ctagtccgac gaggacttgc ttgtctccgg
     9421 attgttggag gaactctcca gggcctcaag atccacaaca gagccttcta gaagactggt
     9481 caataactag ttggtctttg tctgagtctg acttacgagg ttgcatactc gctccctttg
     9541 cctcgtcaat cgatgagaaa aagcgccaaa actcgcaata tggctttgaa ccacacggtg
     9601 ctgagactag ttagaatcta gtcccaaact agcttggata gcttaccttt gccctttgcg
     9661 ttgcgacagg tcttgcaggg tatggttcct ttctcaccag ctgatttagc tgccttgcta
     9721 ccctcacggc ggatctgcat aaagagtggc tagaggttat aaattagcac tgatcctagg
     9781 tacggggctg aatgtaactt gcctttcctt tctcatcgcg cggcaagaca ggcttgctca
     9841 aattcctacc agtcacaggg gtatgcacgg cgtacggacc acttgaacta gtcacagatt
     9901 agttagcaac tagtctgcat tgaatggctg tacttacggg ccctcgccat tgtcctgatc
     9961 atttccagct tcaccctcgt tgctgcaaag tagttagtga ctagtcaagg actagttgaa
    10021 atgggagaag aaactcacga attctcgaca cccttagtat tgtggtcctt ggacttggtg
    10081 ctgctatata ttagctaata cactagttag actcacagaa acttacgcag ctcgcttgcg
    10141 cttcttggta ggagtcgggg ttgggagaac agtgccttca aacaagcctt cataccatgc
    10201 tacttgacta gtcagggact agtcaccaag taatctagat aggacttgcc tttggcctcc
    10261 atcagttcct tcatagtggg aggtccattg tgcaatgtaa actccatgcc gtgggagttc
    10321 ttgtccttca agtgcttgac caatatgttt ctgttggcag agggaacctg tcaactagtt
    10381 aataactagt cagaaactag tatagcagta gactcactgt acgcttgagg catcccttca
    10441 ctcggcagta gacttcatat ggatggatat caggcacgcc attgtcgtcc tgtggactag
    10501 tcagtaacta ggcttaaagc tagtcgggtc ggcttactat cttgaaatcc ggcagcgtaa
    10561 gctccccgtc cttaactgcc tcgagatagt gacagtactc tggggacttt cggagatcgt
    10621 tatcgcgaat gctcggcata ctaatcgttg actagtcttg gactagtccc gagcaaaaag
    10681 gattggagga ggaggaggaa ggtgagagtg agacaaagag cgaaataaga gcttcaaagg
    10741 ctatctctaa gcagtatgaa ggttaagtat ctagttcttg actagattta aaagagattt
    10801 cgactagtta tgtacctgga gtttggatat aggaatgtgt tgtggtaacg aaatgtaagg
    10861 gggaggaaag aaaaagtcgg tcaagaggta actctaagtc ggccattcct ttttgggagg
    10921 cgctaaccat aaacggcatg gtcgacttag agttagctca gggaatttag ggagttatct
    10981 gcgaccactg gactctcagg ctttccattg gggtactaac atagccatca aatgccggcc
    11041 cgtgtttaat ctcctcgaga tggctgggct cacatgcgtt ctactcaaaa actcttatac
    11101 ctaggccctc tcccaatcgt gtatgtttgg accttcctct ctcacacttt ggataacatc
    11161 aagcatactg cagattttga cctagttaac aaggcacatc atgctatcac ttccaacact
    11221 cttgctgagc gcgttctgcc ttgcctttct ggttctacga agtatccagt cccgcctgca
    11281 acgccgcaga aacgccaaaa ggtggggttg tcaaccgcca gctagagctc catcgggatt
    11341 ctttggcatt ccgccgttcc tcggtggtcg cagataactc cctaaattcc ctgagctaac
    11401 tctaagtcga ccatgccgtt tatggttagc gcctcccaaa aaggaatggc cgacttagag
    11461 ttacctcttg accgactttt tctttcctcc cccttacatt tcgttaccac aacacattcc
    11521 tatatccaaa ctccaggtac ataactagtc gaaatctctt ttaaatctag tcaagaacta
    11581 gatacttaac cttcatactg cttagagata gcctttgaag ctcttatttc gctctttgtc
    11641 tcactctcac cttcctcctc ctcctccaat cctttttgct cgggactagt ccaagactag
    11701 tcaacgatta gtatgccgag cattcgcgat aacgatctcc gaaagtcccc agagtactgt
    11761 cactatctcg aggcagttaa ggacggggag cttacgctgc cggatttcaa gatagtaagc
    11821 cgacccgact agctttaagc ctagttactg actagtccac aggacgacaa tggcgtgcct
    11881 gatatccatc catatgaagt ctactgccga gtgaagggat gcctcaagcg tacagtgagt
    11941 ctactgctat actagtttct gactagttat taactagttg acaggttccc tctgccaaca
    12001 gaaacatatt ggtcaagcac ttgaaggaca agaactccca cggcatggag tttacattgc
    12061 acaatggacc tcccactatg aaggaactga tggaggccaa aggcaagtcc tatctagatt
    12121 acttggtgac tagtccctga ctagtcaagt agcatggtat gaaggcttgt ttgaaggcac
    12181 tgttctccca accccgactc ctaccaagaa gcgcaagcga gctgcgtaag tttctgtgag
    12241 tctaactagt gtattagcta atatatagca gcaccaagtc caaggaccac aatactaagg
    12301 gtgtcgagaa ttcgtgagtt tcttctccca tttcaactag tccttgacta gtcactaact
    12361 actttgcagc aacgagggtg aagctggaaa tgatcaggac aatggcgagg gcccgtaagt
    12421 acagccattc aatgcagact agttgctaac taatctgtga ctagttcaag tggtccgtac
    12481 gccgtgcata cccctgtgac tggtaggaat ttgagcaagc ctgtcttgcc gcgcgatgag
    12541 aaaggaaagg caagttacat tcagccccgt acctaggatc agtgctaatt tataacctct
    12601 agccactctt tatgcagatc cgccgtgagg gtagcaaggc agctaaatca gctggtgaga
    12661 aaggaaccat accctgcaag acctgtcgca acgcaaaggg caaaggtaag ctatccaagc
    12721 tagtttggga ctagattcta actagtctca gcaccgtgtg gttcaaagcc atattgcgag
    12781 ttttggcgct ttttctcatc gattgacgag gcaaagggag cgagtatgca acctcgtaag
    12841 tcagactcag acaaagacca actagttatt gaccagtctt ctagaaggct ctgttgtgga
    12901 tcttgaggcc ctggagagtt cctccaacaa tccggagaca agcaagtcct cgtcggacta
    12961 gtcactaact agactctaac tagttgcaga catggataat gcaaaagaga caagcaatga
    13021 agaaagtggt aagacatttc tcctttgtgg ttctggacta gtctttgact agtcacagtc
    13081 ttaaacaagg aaaatgagca tgaaaatgag gaggaaaagg ctgctgagcc cgaggaagtg
    13141 cagggtgatg gcagacatgg taggttaata ccttgttagt tattgctagt cactgactag
    13201 tcaataacta gtctctgaac accttgcaat cactccgttt gcgcagctga acagtggtga
    13261 ggataatagt agtaagttat tctagcttca gagttatagg agactagata ctaactagta
    13321 ttagttgcaa ctaacctgga tctcagagac tttggcctca atctagaatc tatctagttg
    13381 tcaactagac tgtggtatca ttgtctttta ttttcctagt cctggaacta gcttctaact
    13441 agtctcccta atatgtggct gtcttgtttt ttttttttgt ttccctaccc ggatatctag
    13501 tccccttcta ggttctgtta acctctcggg ctctgattta gtttaacgca aacctgagat
    13561 tagtttctaa ctagtctcta ggttttctat ccacctttaa ttgtaataat aaatacaagc
    13621 aacgtttata cgtcaaaagc atttataaac ttttacccta aagtagcttg cttgtgtgtt
    13681 tagtttataa ttagtctctt attaatttga tgtaggtaag cccgccacaa atatatattt
    13741 ttaacaagat accgtggaaa aacttcgtgc tatcacaaaa cagtatacaa aaaataagct
    13801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    13861 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
    13921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    13981 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
    14041 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    14101 tttaacaaaa tggggtgacg atgagccgct cttgcatctt tgtttgtatt atactgtctt
    14161 tcttgttaca cataattatt ctagaatgcc ccaccgttac atacgggaca cagccattta
    14221 catatgcatg tggattacga gctaacgagt tcattcaaat ctcagaacta tcacataatc
    14281 atcattcccc tatcgtcaaa gaccgtaaga caaatccggt tcatgcactg aacccattcg
    14341 ggtagtgagt catttactca gcacactcgc gctgacgctc gtcgaacacc ttcaatgcct
    14401 cctcggcagc cttgacacca ctgagaacca tggcaccgaa ggtagggccc atgcggttaa
    14461 agccatcaat ttcagacagc tccataccgc cgattatcaa gcccttagta acctcgcggg
    14521 tgttcttgac gatggcatcc tgcgctgatt gtgatagttc ccacttgtcc gtccgcatcg
    14581 gcatccgcag ctcgggatag ttccgaccta ggattggatg catgcggaac cgcacgaggg
    14641 cggggcggaa attgacacac cactcctctc cacgcaccgt tcaagaggta cgcgtataga
    14701 gccgtataga gcagagacgg agcactttct ggtactgtcc gcacgggatg tccgcacgga
    14761 gagccacaaa cgagcggggc cccgtacgtg ctctcctacc ccaggatcgc atccccgcat
    14821 agctgaacat ctatataaag acccccaagg ttctcagtct caccaacatc atcaaccaac
    14881 aatcaacagt tctctactca gttaattaga actcttccaa tcctatcacc tcgccttaat
    14941 taagcggaac aaattgaagc gagtccccgt ggagcgcgag cccgcgagag agtcgtgcgg
    15001 gatgatgcca tgaagtatca cctacattgt atgctctcca gatcgaagag gaatactctg
    15061 aataagtgga tttaggcggt ttctttggtc ctactatatg cacactcaga ttctgtcaat
    15121 tgaaaagtca gcccgctgtc tatcatgact tctatctcca ttaattgtca tccagccgtg
    15181 cctctgtatg gtgcgtatac cctcgactca cacctgtgaa gtctctgcca cttagcgcag
    15241 ccaaagaaga tttagtctaa aggtctacaa tcaattcagg ccgtattcag ggcggccgct
    15301 tctgctgttt ctgggctcaa tgctttggta tctcaaagga gaggagaagc gtccgtggaa
    15361 ggcaggaaga cctacaagac ctggcactca gaaagagggc ggttgctctt aagtgtaggg
    15421 ctgcgcaggg gaggaactct ggggttgtca aatgcggcag acggaaatct acattagcag
    15481 cgtcaatcca cccccttttg gtgaaccata aaggggctag tctaccccag cttcgaatca
    15541 gaataatata aatgcgggga aagctttagg tatgcggaca gatccatact gggtcctcca
    15601 agagatcggc ccgcacatct gacgtggttt caaggttcga cccatgcgga gccgcattgg
    15661 aggatctatc cctgtaggta ccttagctaa agatctacgc cgtatggcca agcggaagat
    15721 ataggaacga tcggccatga agtcgtgtct tctacaaact ctcgtatatt cactaggttc
    15781 gacgtctgct aaactaacct gacctaacct aaccttaact ccctgccagt tcagcccatg
    15841 cggacccggc agtcatgtct ttcgcttcag attgaacgcg gctgcctcgt ctcgatcagg
    15901 atttagcaga agacagtaca cactgaaaaa gtaccacaat aaagaagcta tattgcaggc
    15961 caaatagccg tctgctcggt ctgtttattc aacctggcct ctttcatcgt ttttcccttt
    16021 tccttattaa accatatatc tccagcagag cgatcgcggg aggagataat ttagttgaga
    16081 tcgaagttgg ctttatagtt gtaacagtga aatcagggca cgaatcttta gtacttcgtt
    16141 gatcgccact ccaccaaggg tttgccatta agcataactg gcaatatttt tgtcaatttg
    16201 ccttttcgac cgtgtaaatg tccctatcca tactctccgc agtcaatatc cggaggtcaa
    16261 gtgtgaagac tgtttacccc agcaagatac ttggacaagc ctggtttgca atccatagtt
    16321 tcagcaggct tcctaggtgg ccttaagcct gggatactgg ctggctgcta gcaattgcca
    16381 tgactatgtt ggaggaaatg ttcgagagga atcttgggtc catgaacaga gtagtcccac
    16441 ttcaaatgtt gcgtatgcct gatattacgg accaatagag gaacaacctg gcagcagcta
    16501 acttgtatat ctctaatcgc gttgacgacc gttctgtgtc agtgcaactg ccacgcaaag
    16561 acttcagaat aataaacttt gccagagaag gcagttgtat tatgttaata tcgcaacatt
    16621 gtgaaaatta aatgaactaa caatcaaaaa gaaagaaaat ccgaactttc cctccgtatt
    16681 atctaacccg tgtgcttttg caacgcaatc caactattcc agttgacgac attaatccaa
    16741 gccagcatag gaagaatacc aaaaaccttc agacagccgt acccgcaagc cttgttacca
    16801 accagctata gcaatcttac ccgttcaatt tcttaatccc catctagacg cttccagcac
    16861 tctgactgtc gggagacggg ttcctttgcc agccatgagc attcgcctag ttagggagcg
    16921 gcggatgcct acttacttct gggaacgagg cgcgccgtga gcgggaaata tgggtatctt
    16981 gggagaggag agttggagta gggagaaatg ctggctcggt gggaggacga gggagcttta
    17041 ataataccgc tctggctttg gtagcactga gccagtggtc gcagcaaaag aggggaaagg
    17101 aggggaacag gtcaagcgga gccgctcttg ttgaggatag ctgttaccca tggaaattac
    17161 atgtagcaag ctagcaaccc gccgaagtgc cccactggtc ttcaagagcc agtccaggtg
    17221 aactgcattc cgtccccttc gccgcggtgc ccactgcctc cacagcctag atgattctga
    17281 aaacacgttg aagagctcgc gataataacc gaggcgggta atgcaggatg aaacttacga
    17341 tctacaacct acagcaccct ggttctgaaa caaagttgag gatccccgcg atcgataacc
    17401 cagaccgtaa tgcgaattgc aatttacgat ttaccccccg tagagcggac tgattctgga
    17461 cctatcttga gactatgtgg tggagcctgc gatgataacc gaggccgtaa tgcaaaaatg
    17521 aaaattacga accaagaggc gtgatgagaa gatca
//
