LOCUS       sequence-350002-       20651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression the full-length ectodomain of human LRP1 [residue 1-4414]
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Bang YJ, Hu Z, Li Y, Gattu S, Ruhn KA, Raj P, Herz J, Hooper LV
  TITLE     Serum amyloid A delivers retinol to intestinal myeloid cells to 
            promote adaptive immunity.
  JOURNAL   Science. 2021 Sep 17;373(6561):eabf9232. doi: 
            10.1126/science.abf9232. Epub 2021 Sep 17.
  PUBMED    34529485
REFERENCE   2  (bases 1 to 20651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-09-17"; volume: "373(6561):eabf9232. doi"; pages: " 
            10.1126/science.abf9232. Epub 2021 Sep 17"
COMMENT     Sequence Label: hLRP1-pEZT-BM
FEATURES             Location/Qualifiers
     source          1..20651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          12..144
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     127..146
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13498..14086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13551..13571)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13969..13980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    14228..14362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14265..14284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14319..14338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  14391..14556
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      14740..15194
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14827..14846)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15036..15057
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15220..15324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15325..15393,15394..16185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15543..15562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16356..16944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16845..16864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17098..17115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  17249..17473
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(17540..18073)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(18243..18248,18249..18265,18266..18271))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    18779..18827
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     18852..18871
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(18912..19622,19623..19625))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(18954..18975)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     19303..19322
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     19562..19583
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(19650..19759)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     enhancer        19944..20323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        20324..20527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     20478..20498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     20524..20548
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg
       61 gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca
      121 tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc tcttaaggct
      181 agagtactta atacgactca ctataggcta gatgctgacc ccgccgttgc tcctgctgct
      241 gcccctgctc tcagctctgg tcgcggcggc tatcgacgcc cctaagactt gcagccccaa
      301 gcagtttgcc tgcagagatc aaataacctg tatctcaaag ggctggcggt gcgacggtga
      361 gagggactgc ccagacggat ctgacgaggc ccctgagatt tgtccacaga gtaaggccca
      421 gcgatgccag ccaaacgagc ataactgcct gggtactgag ctgtgtgttc ccatgtcccg
      481 cctctgcaat ggggtccagg actgcatgga cggctcagat gaggggcccc actgccgaga
      541 gctccaaggc aactgctctc gcctgggctg ccagcaccat tgtgtcccca cactcgatgg
      601 gcccacctgc tactgcaaca gcagctttca gcttcaggca gatggcaaga cctgcaaaga
      661 ttttgatgag tgctcagtgt acggcacctg cagccagcta tgcaccaaca cagacggctc
      721 cttcatatgt ggctgtgttg aaggatacct cctgcagccg gataaccgct cctgcaaggc
      781 caagaacgag ccagtagacc ggccccctgt gctgttgata gccaactccc agaacatctt
      841 ggccacgtac ctgagtgggg cccaggtgtc taccatcaca cctacgagca cgcggcagac
      901 cacagccatg gacttcagct atgccaacga gaccgtatgc tgggtgcatg ttggggacag
      961 tgctgctcag acgcagctca agtgtgcccg catgcctggc ctaaagggct tcgtggatga
     1021 gcacaccatc aacatctccc tcagtctgca ccacgtggaa cagatggcca tcgactggct
     1081 gacaggcaac ttctactttg tggatgacat cgatgatagg atctttgtct gcaacagaaa
     1141 tggggacaca tgtgtcacat tgctagacct ggaactctac aaccccaagg gcattgccct
     1201 ggaccctgcc atggggaagg tgtttttcac tgactatggg cagatcccaa aggtggaacg
     1261 ctgtgacatg gatgggcaga accgcaccaa gctcgtcgac agcaagattg tgtttcctca
     1321 tggcatcacg ctggacctgg tcagccgcct tgtctactgg gcagatgcct atctggacta
     1381 tattgaagtg gtggactatg agggcaaggg ccgccagacc atcatccagg gcatcctgat
     1441 tgagcacctg tacggcctga ctgtgtttga gaattatctc tatgccacca actcggacaa
     1501 tgccaatgcc cagcagaaga cgagtgcgat ccgtgtgaac cgctttaaca gcaccgagta
     1561 ccaggttgtc acccgggtgg acaagggtgg tgccctccac atctaccacc agaggcgtca
     1621 gccccgagtg aggagccatg cctgtgaaaa cgaccagtat gggaagccgg gtggctgctc
     1681 tgacatctgc ctgctggcca acagccacaa ggcgcggacc tgccgctgcc gttccggctt
     1741 cagcctgggc agtgacggga agtcatgcaa gaagccggag catgagctgt tcctcgtgta
     1801 tggcaagggc cggccaggca tcatccgggg catggatatg ggggccaagg tcccggatga
     1861 gcacatgatc cccattgaaa acctcatgaa cccccgagcc ctggacttcc acgctgagac
     1921 cggcttcatc tactttgccg acaccaccag ctacctcatt ggccgccaga agattgatgg
     1981 cactgagcgg gagaccatcc tgaaggacgg catccacaat gtggagggtg tggccgtgga
     2041 ctggatggga gacaatctgt actggacgga cgatgggccc aaaaagacaa tcagcgtggc
     2101 caggctggag aaagctgctc agacccgcaa gactttaatc gagggcaaaa tgacacaccc
     2161 cagggctatt gtggtggatc cactcaatgg gtggatgtac tggacagact gggaggagga
     2221 ccccaaggac agtcggcgtg ggcggctgga gagggcgtgg atggatggct cacaccgaga
     2281 catctttgtc acctccaaga cagtgctttg gcccaatggg ctaagcctgg acatcccggc
     2341 tgggcgcctc tactgggtgg atgccttcta cgaccgcatc gagacgatac tgctcaatgg
     2401 cacagaccgg aagattgtgt atgaaggtcc tgagctgaac cacgcctttg gcctgtgtca
     2461 ccatggcaac tacctcttct ggactgagta tcggagtggc agtgtctacc gcttggaacg
     2521 gggtgtagga ggcgcacccc ccactgtgac ccttctgcgc agtgagcggc cccccatctt
     2581 tgagatccga atgtatgatg cccagcagca gcaagttggc accaacaaat gccgggtgaa
     2641 caatggcggc tgcagcagcc tgtgcttggc cacccctggg agccgccagt gcgcctgtgc
     2701 tgaggaccag gtgttggacg cagacggcgt cacttgcttg gcgaacccat cctacgtgcc
     2761 tccaccccag tgccagccag gcgagtttgc ctgtgccaac agccgctgca tccaggagcg
     2821 ctggaagtgt gacggagaca acgattgcct ggacaacagt gatgaggccc cagccctctg
     2881 ccatcagcac acctgcccct cggaccgatt caagtgcgag aacaaccggt gcatccccaa
     2941 ccgctggctc tgcgacgggg acaatgactg tgggaacagt gaagatgagt ccaatgccac
     3001 ttgttcagcc cgcacctgcc cccccaacca gttctcctgt gccagtggcc gctgcatccc
     3061 catctcctgg acgtgtgatc tggatgacga ctgtggggac cgctctgatg agtctgcttc
     3121 gtgtgcctat cccacctgct tccccctgac tcagtttacc tgcaacaatg gcagatgtat
     3181 caacatcaac tggagatgcg acaatgacaa tgactgtggg gacaacagtg acgaagccgg
     3241 ctgcagccac tcctgttcta gcacccagtt caagtgcaac agcgggcgtt gcatccccga
     3301 gcactggacc tgcgatgggg acaatgactg cggagactac agtgatgaga cacacgccaa
     3361 ctgcaccaac caggccacga ggccccctgg tggctgccac actgatgagt tccagtgccg
     3421 gctggatgga ctatgcatcc ccctgcggtg gcgctgcgat ggggacactg actgcatgga
     3481 ctccagcgat gagaagagct gtgagggagt gacccacgtc tgcgatccca gtgtcaagtt
     3541 tggctgcaag gactcagctc ggtgcatcag caaagcgtgg gtgtgtgatg gcgacaatga
     3601 ctgtgaggat aactcggacg aggagaactg cgagtccctg gcctgcaggc caccctcgca
     3661 cccttgtgcc aacaacacct cagtctgcct gccccctgac aagctgtgtg atggcaacga
     3721 cgactgtggc gacggctcag atgagggcga gctctgtgac cagtgctctc tgaataacgg
     3781 tggctgcagc cacaactgct cagtggcacc tggcgaaggc attgtgtgtt cctgccctct
     3841 gggcatggag ctggggcccg acaaccacac ctgccagatc cagagctact gtgccaagca
     3901 tctcaaatgc agccaaaagt gcgaccagaa caagttcagc gtgaagtgct cctgctacga
     3961 gggctgggtc ctggaacctg acggcgagag ctgccgcagc ctggacccct tcaagccgtt
     4021 catcattttc tccaaccgcc atgaaatccg gcgcatcgat cttcacaaag gagactacag
     4081 cgtcctggtg cccggcctgc gcaacaccat cgccctggac ttccacctca gccagagcgc
     4141 cctctactgg accgacgtgg tggaggacaa gatctaccgc gggaagctgc tggacaacgg
     4201 agccctgact agtttcgagg tggtgattca gtatggcctg gccacacccg agggcctggc
     4261 tgtagactgg attgcaggca acatctactg ggtggagagt aacctggatc agatcgaggt
     4321 ggccaagctg gatgggaccc tccggaccac cctgctggcc ggtgacattg agcacccaag
     4381 ggcaatcgca ctggatcccc gggatgggat cctgttttgg acagactggg atgccagcct
     4441 gccccgcatt gaggcagcct ccatgagtgg ggctgggcgc cgcaccgtgc accgggagac
     4501 cggctctggg ggctggccca acgggctcac cgtggactac ctggagaagc gcatcctttg
     4561 gattgacgcc aggtcagatg ccatttactc agcccgttac gacggctctg gccacatgga
     4621 ggtgcttcgg ggacacgagt tcctgtcgca cccgtttgca gtgacgctgt acggggggga
     4681 ggtctactgg actgactggc gaacaaacac actggctaag gccaacaagt ggaccggcca
     4741 caatgtcacc gtggtacaga ggaccaacac ccagcccttt gacctgcagg tgtaccaccc
     4801 ctcccgccag cccatggctc ccaatccctg tgaggccaat gggggccagg gcccctgctc
     4861 ccacctgtgt ctcatcaact acaaccggac cgtgtcctgc gcctgccccc acctcatgaa
     4921 gctccacaag gacaacacca cctgctatga gtttaagaag ttcctgctgt acgcacgtca
     4981 gatggagatc cgaggtgtgg acctggatgc tccctactac aactacatca tctccttcac
     5041 ggtgcccgac atcgacaacg tcacagtgct agactacgat gcccgcgagc agcgtgtgta
     5101 ctggtctgac gtgcggacac aggccatcaa gcgggccttc atcaacggca caggcgtgga
     5161 gacagtcgtc tctgcagact tgccaaatgc ccacgggctg gctgtggact gggtctcccg
     5221 aaacctgttc tggacaagct atgacaccaa taagaagcag atcaatgtgg cccggctgga
     5281 tggctccttc aagaacgcag tggtgcaggg cctggagcag ccccatggcc ttgtcgtcca
     5341 ccctctgcgt gggaagctct actggaccga tggtgacaac atcagcatgg ccaacatgga
     5401 tggcagcaat cgcaccctgc tcttcagtgg ccagaagggc cccgtgggcc tggctattga
     5461 cttccctgaa agcaaactct actggatcag ctccgggaac cataccatca accgctgcaa
     5521 cctggatggg agtgggctgg aggtcatcga tgccatgcgg agccagctgg gcaaggccac
     5581 cgccctggcc atcatggggg acaagctgtg gtgggctgat caggtgtcgg aaaagatggg
     5641 cacatgcagc aaggctgacg gctcgggctc cgtggtcctt cggaacagca ccaccctggt
     5701 gatgcacatg aaggtctatg acgagagcat ccagctggac cataagggca ccaacccctg
     5761 cagtgtcaac aacggtgact gctcccagct ctgcctgccc acgtcagaga cgacccgctc
     5821 ctgcatgtgc acagccggct atagcctccg gagtggccag caggcctgcg agggcgtagg
     5881 ttcctttctc ctgtactctg tgcatgaggg aatcagggga attcccctgg atcccaatga
     5941 caagtcagat gccctggtcc cagtgtccgg gacctcgctg gctgtcggca tcgacttcca
     6001 cgctgaaaat gacaccatct actgggtgga catgggcctg agcacgatca gccgggccaa
     6061 gcgggaccag acgtggcgtg aagacgtggt gaccaatggc attggccgtg tggagggcat
     6121 tgcagtggac tggatcgcag gcaacatcta ctggacagac cagggctttg atgtcatcga
     6181 ggtcgcccgg ctcaatggct ccttccgcta cgtggtgatc tcccagggtc tagacaagcc
     6241 ccgggccatc accgtccacc cggagaaagg gtacttgttc tggactgagt ggggtcagta
     6301 tccgcgtatt gagcggtctc ggctagatgg cacggagcgt gtggtgctgg tcaacgtcag
     6361 catcagctgg cccaacggca tctcagtgga ctaccaggat gggaagctgt actggtgcga
     6421 tgcacggaca gacaagattg aacggatcga cctggagaca ggtgagaacc gcgaggtggt
     6481 tctgtccagc aacaacatgg acatgttttc agtgtctgtg tttgaggatt tcatctactg
     6541 gagtgacagg actcatgcca acggctctat caagcgcggg agcaaagaca atgccacaga
     6601 ctccgtgccc ctgcgaaccg gcatcggcgt ccagcttaaa gacatcaaag tcttcaaccg
     6661 ggaccggcag aaaggcacca acgtgtgcgc ggtggccaat ggcgggtgcc agcagctgtg
     6721 cctgtaccgg ggccgtgggc agcgggcctg cgcctgtgcc cacgggatgc tggctgaaga
     6781 cggagcatcg tgccgcgagt atgccggcta cctgctctac tcagagcgca ccattctcaa
     6841 gagtatccac ctgtcggatg agcgcaacct caatgcgccc gtgcagccct tcgaggaccc
     6901 tgagcacatg aagaacgtca tcgccctggc ctttgactac cgggcaggca cctctccggg
     6961 cacccccaat cgcatcttct tcagcgacat ccactttggg aacatccaac agatcaacga
     7021 cgatggctcc aggaggatca ccattgtgga aaacgtgggc tccgtggaag gcctggccta
     7081 tcaccgtggc tgggacactc tctattggac aagctacacg acatccacca tcacgcgcca
     7141 cacagtggac cagacccgcc caggggcctt cgagcgtgag accgtcatca ctatgtctgg
     7201 agatgaccac ccacgggcct tcgttttgga cgagtgccag aacctcatgt tctggaccaa
     7261 ctggaatgag cagcatccca gcatcatgcg ggcggcgctc tcgggagcca atgtcctgac
     7321 ccttatcgag aaggacatcc gtacccccaa tggcctggcc atcgaccacc gtgccgagaa
     7381 gctctacttc tctgacgcca ccctggacaa gatcgagcgg tgcgagtatg acggctccca
     7441 ccgctatgtg atcctaaagt cagagcctgt ccaccccttc gggctggccg tgtatgggga
     7501 gcacattttc tggactgact gggtgcggcg ggcagtgcag cgggccaaca agcacgtggg
     7561 cagcaacatg aagctgctgc gcgtggacat cccccagcag cccatgggca tcatcgccgt
     7621 ggccaacgac accaacagct gtgaactctc tccatgccga atcaacaacg gtggctgcca
     7681 ggacctgtgt ctgctcactc accagggcca tgtcaactgc tcatgccgag ggggccgaat
     7741 cctccaggat gacctcacct gccgagcggt gaattcctct tgccgagcac aagatgagtt
     7801 tgagtgtgcc aatggcgagt gcatcaactt cagcctgacc tgcgacggcg tcccccactg
     7861 caaggacaag tccgatgaga agccatccta ctgcaactcc cgccgctgca agaagacttt
     7921 ccggcagtgc agcaatgggc gctgtgtgtc caacatgctg tggtgcaacg gggccgacga
     7981 ctgtggggat ggctctgacg agatcccttg caacaagaca gcctgtggtg tgggcgagtt
     8041 ccgctgccgg gacgggacct gcatcgggaa ctccagccgc tgcaaccagt ttgtggattg
     8101 tgaggacgcc tcagatgaga tgaactgcag tgccaccgac tgcagcagct acttccgcct
     8161 gggcgtgaag ggcgtgctct tccagccctg cgagcggacc tcactctgct acgcacccag
     8221 ctgggtgtgt gatggcgcca atgactgtgg ggactacagt gatgagcgcg actgcccagg
     8281 tgtgaaacgc cccagatgcc ctctgaatta cttcgcctgc cctagtgggc gctgcatccc
     8341 catgagctgg acgtgtgaca aagaggatga ctgtgaacat ggcgaggacg agacccactg
     8401 caacaagttc tgctcagagg cccagtttga gtgccagaac catcgctgca tctccaagca
     8461 gtggctgtgt gacggcagcg atgactgtgg ggatggctca gacgaggctg ctcactgtga
     8521 aggcaagacg tgcggcccct cctccttctc ctgccctggc acccacgtgt gcgtccccga
     8581 gcgctggctc tgtgacggtg acaaagactg tgctgatggt gcagacgaga gcatcgcagc
     8641 tggttgcttg tacaacagca cttgtgacga ccgtgagttc atgtgccaga accgccagtg
     8701 catccccaag cacttcgtgt gtgaccacga ccgtgactgt gcagatggct ctgatgagtc
     8761 ccccgagtgt gagtacccga cctgtggccc cagtgagttc cgctgtgcca atgggcgctg
     8821 tctgagctcc cgccagtggg agtgtgatgg cgagaatgac tgccacgacc agagtgacga
     8881 ggctcccaag aacccacact gcaccagccc agagcacaag tgcaatgcct cgtcacagtt
     8941 cctgtgcagc agtgggcgct gtgtggctga ggcactgctc tgcaacggcc aggatgactg
     9001 tggcgacagc tcggacgagc gtggctgcca catcaatgag tgtctcagcc gcaagctcag
     9061 tggctgcagc caggactgtg aggacctcaa gatcggcttc aagtgccgct gtcgccctgg
     9121 cttccggctg aaggatgacg gccggacgtg tgctgatgtg gacgagtgca gcaccacctt
     9181 cccctgcagc cagcgctgca tcaacaccca tggcagctat aagtgtctgt gtgtggaggg
     9241 ctatgcaccc cgcggcggcg acccccacag ctgcaaggct gtgactgacg aggaaccgtt
     9301 tctgatcttc gccaaccggt actacctgcg caagctcaac ctggacgggt ccaactacac
     9361 gttacttaag cagggcctga acaacgccgt tgccttggat tttgactacc gagagcagat
     9421 gatctactgg acagatgtga ccacccaggg cagcatgatc cgaaggatgc accttaacgg
     9481 gagcaatgtg caggtcctac accgtacagg cctcagcaac cccgatgggc tggctgtgga
     9541 ctgggtgggt ggcaacctgt actggtgcga caaaggccgg gacaccatcg aggtgtccaa
     9601 gctcaatggg gcctatcgga cggtgctggt cagctctggc ctccgtgagc ccagggctct
     9661 ggtggtggat gtgcagaatg ggtacctgta ctggacagac tggggtgacc attcactgat
     9721 cggccgcatc ggcatggatg ggtccagccg cagcgtcatc gtggacacca agatcacatg
     9781 gcccaatggc ctgacgctgg actatgtcac tgagcgcatc tactgggccg acgcccgcga
     9841 ggactacatt gaatttgcca gcctggatgg ctccaatcgc cacgttgtgc tgagccagga
     9901 catcccgcac atctttgcac tgaccctgtt tgaggactac gtctactgga ccgactggga
     9961 aacaaagtcc attaaccgag cccacaagac cacgggcacc aacaaaacgc tcctcatcag
    10021 cacgctgcac cggcccatgg acctgcatgt cttccatgcc ctgcgccagc cagacgtgcc
    10081 caatcacccc tgcaaggtca acaatggtgg ctgcagcaac ctgtgcctgc tgtcccccgg
    10141 gggagggcac aaatgtgcct gccccaccaa cttctacctg ggcagcgatg ggcgcacctg
    10201 tgtgtccaac tgcacggcta gccagtttgt atgcaagaac gacaagtgca tccccttctg
    10261 gtggaagtgt gacaccgagg acgactgcgg ggaccactca gacgagcccc cggactgccc
    10321 tgagttcaag tgccggcccg gacagttcca gtgctccaca ggtatctgca caaaccctgc
    10381 cttcatctgc gatggcgaca atgactgcca ggacaacagt gacgaggcca actgtgacat
    10441 ccacgtctgc ttgcccagtc agttcaaatg caccaacacc aaccgctgta ttcccggcat
    10501 cttccgctgc aatgggcagg acaactgcgg agatggggag gatgagaggg actgccccga
    10561 ggtgacctgc gcccccaacc agttccagtg ctccattacc aaacggtgca tcccccgggt
    10621 ctgggtctgc gaccgggaca atgactgtgt ggatggcagt gatgagcccg ccaactgcac
    10681 ccagatgacc tgtggtgtgg acgagttccg ctgcaaggat tcgggccgct gcatcccagc
    10741 gcgttggaag tgtgacggag aggatgactg tggggatggc tcggatgagc ccaaggaaga
    10801 gtgtgatgaa cgcacctgtg agccatacca gttccgctgc aagaacaacc gctgcgtgcc
    10861 cggccgctgg cagtgcgact acgacaacga ttgcggtgac aactccgatg aagagagctg
    10921 cacccctcgg ccctgctccg agagtgagtt ctcctgtgcc aacggccgct gcatcgcggg
    10981 gcgctggaaa tgcgatggag accacgactg cgcggacggc tcggacgaga aagactgcac
    11041 cccccgctgt gacatggacc agttccagtg caagagcggc cactgcatcc ccctgcgctg
    11101 gcgctgtgac gcagacgccg actgcatgga cggcagcgac gaggaggcct gcggcactgg
    11161 cgtgcggacc tgccccctgg acgagttcca gtgcaacaac accttgtgca agccgctggc
    11221 ctggaagtgc gatggcgagg atgactgtgg ggacaactca gatgagaacc ccgaggagtg
    11281 tgcccggttc gtgtgccctc ccaaccggcc cttccgttgc aagaatgacc gcgtctgtct
    11341 gtggatcggg cgccaatgcg atggcacgga caactgtggg gatgggactg atgaagagga
    11401 ctgtgagccc cccacagccc acaccaccca ctgcaaagac aagaaggagt ttctgtgccg
    11461 gaaccagcgc tgcctctcct cctccctgcg ctgcaacatg ttcgatgact gcggggacgg
    11521 ctctgacgag gaggactgca gcatcgaccc caagctgacc agctgcgcca ccaatgccag
    11581 catctgtggg gacgaggcac gctgcgtgcg caccgagaaa gcggcctact gtgcctgccg
    11641 ctcgggcttc cacaccgtgc ccggccagcc cggatgccaa gacatcaacg agtgcctgcg
    11701 cttcggcacc tgctcccagc tctgcaacaa caccaagggc ggccacctct gcagctgcgc
    11761 tcggaacttc atgaagacgc acaacacctg caaggccgaa ggctctgagt accaggtcct
    11821 gtacatcgct gatgacaatg agatccgcag cctgttcccc ggccaccccc attcggctta
    11881 cgagcaggca ttccagggtg acgagagtgt ccgcattgat gctatggatg tccatgtcaa
    11941 ggctggccgt gtctattgga ccaactggca cacgggcacc atctcctacc gcagcctgcc
    12001 acctgctgcg cctcctacca cttccaaccg ccaccggcga cagattgacc ggggtgtcac
    12061 ccacctcaac atttcagggc tgaagatgcc cagaggcatc gccatcgact gggtggccgg
    12121 aaacgtgtac tggaccgact cgggccgaga tgtgattgag gtggcgcaga tgaagggcga
    12181 gaaccgcaag acgctcatct cgggcatgat tgacgagccc cacgccattg tggtggaccc
    12241 actgaggggg accatgtact ggtcagactg gggcaaccac cccaagattg agacggcagc
    12301 gatggatggg acgcttcggg agacactggt gcaggacaac attcagtggc ccacaggcct
    12361 ggccgtggat tatcacaatg agcggctgta ctgggcagac gccaagcttt cagtcatcgg
    12421 cagcatccgg ctcaatggca cggaccccat tgtggctgct gacagcaaac gaggcctaag
    12481 tcaccccttc agcatcgacg tctttgagga ttacatctat ggtgtcacct acatcaataa
    12541 tcgtgtcttc aagatccata agtttggcca cagccccttg gtcaacctga cagggggcct
    12601 gagccacgcc tctgacgtgg tcctttacca tcagcacaag cagcccgaag tgaccaaccc
    12661 atgtgaccgc aagaaatgcg agtggctctg cctgctgagc cccagtgggc ctgtctgcac
    12721 ctgtcccaat gggaagcggc tggacaacgg cacatgcgtg cctgtgccct ctccaacgcc
    12781 ccccccagat gctccccggc ctggaacctg taacctgcag tgcttcaacg gtggcagctg
    12841 tttcctcaat gcacggaggc agcccaagtg ccgctgccaa ccccgctaca cgggtgacaa
    12901 gtgtgaactg gaccagtgct gggagcactg tcgcaatggg ggcacctgtg ctgcctcccc
    12961 ctctggcatg cccacgtgcc ggtgccccac gggcttcacg ggccccaaat gcacccagca
    13021 ggtgtgtgcg ggctactgtg ccaacaacag cacctgcact gtcaaccagg gcaaccagcc
    13081 ccagtgccga tgcctacccg gcttcctggg cgaccgctgc cagtaccggc agtgctctgg
    13141 ctactgtgag aactttggca catgccagat ggctgctgat ggctcccgac aatgccgctg
    13201 cactgcctac tttgagggat cgaggtgtga ggtgaacaag tgcagccgct gtctcgaagg
    13261 ggcctgtgtg gtcaacaagc agagtgggga tgtcacctgc aactgcacgg atggccgggt
    13321 ggcccccagc tgtctgacct gcgtcggcca ctgcagcaat ggcggctcct gtaccatgaa
    13381 cagcaaaatg atgcctgagt gccagtgccc accccacatg acagggcccc ggtgtgagga
    13441 gcacgtcttc agctgatcga ggcggccgcg actgcaggga tccgaggtac caagcttaat
    13501 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
    13561 tttacgctat gtgaatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
    13621 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    13681 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    13741 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    13801 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    13861 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
    13921 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    13981 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
    14041 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgc agtctgacaa
    14101 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
    14161 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    14221 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    14281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14341 tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa
    14401 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
    14461 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
    14521 acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat
    14581 gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt
    14641 ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact
    14701 gaatatcaac gcttatttgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    14761 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    14821 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    14881 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    14941 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    15001 ttcgcccttt gacgttggag tccacgttct taatagtgga ctcttgttcc aaactggaac
    15061 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    15121 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    15181 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    15241 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    15301 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    15361 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    15421 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    15481 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    15541 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    15601 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    15661 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    15721 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    15781 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    15841 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    15901 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    15961 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    16021 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    16081 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    16141 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    16201 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    16261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    16321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    16381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    16441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    16501 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    16561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    16621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    16681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    16741 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    16801 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    16861 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    16921 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    16981 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    17041 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    17101 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
    17161 tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt tacggttgag
    17221 taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca
    17281 aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa
    17341 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
    17401 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatggt
    17461 aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga
    17521 tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt
    17581 cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt
    17641 gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg
    17701 cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct
    17761 cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt
    17821 cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat
    17881 cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat
    17941 caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc
    18001 ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
    18061 tgctgcgtaa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
    18121 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
    18181 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
    18241 acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg
    18301 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg
    18361 cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct
    18421 tctacggcaa ggtgctgtgc acggatctgc ccttgcttca ggagatcggt agacctcggc
    18481 cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc
    18541 tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt ggttggctac
    18601 gtacccgtag tggctatggc agggcttgcc gccccgacgt tggctgcgag ccctgggcct
    18661 tcacccgaac ttgggggttg gggtggggaa aaggaagaaa cgcgggcgta ttggtcccaa
    18721 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
    18781 acaaacgacc caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
    18841 tccttccggt attgtctcct tccgtgtttc agttagcctc ccccatctcc cggttccgca
    18901 tgctatgcat ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
    18961 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg
    19021 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
    19081 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
    19141 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
    19201 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
    19261 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
    19321 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
    19381 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
    19441 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
    19501 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
    19561 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
    19621 ctcaccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt tacagtatag
    19681 tattttaatt aatatacaaa tgatttgata ataattctta tttaactata atatattgtg
    19741 ttgggttgaa ttaaaggtcc gtatactccg gaatattaat aggatcccgg tccgagcgcg
    19801 cggaattcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat
    19861 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
    19921 atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat
    19981 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    20041 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    20101 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    20161 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
    20221 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
    20281 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    20341 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    20401 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    20461 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    20521 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
    20581 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag
    20641 ttggtcgtga g
//
