LOCUS       sequence-350006-        9256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Detection of Streptomyces coelicolor butyrolactones (SCB) by 
            kanamycin bioassay. Streptomyces genomic integrative vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Biarnes-Carrera M, Breitling R, Takano E
  TITLE     Detection and Quantification of Butyrolactones from Streptomyces.
  JOURNAL   Methods Mol Biol. 2018;1673:117-128. doi: 
            10.1007/978-1-4939-7309-5_10.
  PUBMED    29130169
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1673:117-128. doi"; pages: " 
            10.1007/978-1-4939-7309-5_10"
COMMENT     Sequence Label: pTE1062
FEATURES             Location/Qualifiers
     source          1..9256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(632..649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(803..1391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(883..902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2078..2449)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2482..2591
                     /note="incP origin of transfer"
     CDS             complement(2829..2840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2911..3010
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             3020..4837
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     primer_bind     5265..5287
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5279..5296
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5280..5296
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6740..7534
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDVAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6794..6813)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7404..7423
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7582..7598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7606..7622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7630..7636,7637..7654,7655..7660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7675..7696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7892..7913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7928..7933,7934..7951,7952..7958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7966..7982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7990..8006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggagcccc gcccggcggc ccgggaaccc cgggcccgcg ccgaagcccg ctgctgcgag
       61 cccggagcgg gccggccgac ggcggtgcgg gcccggcggc ggacgctcag cagcggcggg
      121 cgtgaaaggc cctggcatcc tcgatcatct cctccagggt gggtcggccg ggcttccatc
      181 ccagctcggc aaggatcgat ccgcgcagat cagttggaag aatttgtcca ctacgtgaaa
      241 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
      301 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
      361 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
      421 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
      481 cgcaacatcc gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt
      541 ttgaatgacc tttaatagat tatattacta attaatgaat cggccaacgc gcggggagag
      601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1501 tggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
     1561 gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt
     1621 cgtgcaatac gaatggcgaa aagccgagct catgagcgga gaacgagatg acgttggagg
     1681 ggcaaggtcg cgctgattgc tggggcaaca cgtggagcgg atcggggatt gtctttcttc
     1741 agctcgctga tgatatgctg acgctcaatg ccgtttggcc tccgactaac gaaaatcccg
     1801 catttggacg gctgatccga ttggcacggc ggacggcgaa tggcggagca gacgctcgtc
     1861 cgggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtatcgggc cctggccagc
     1921 tagctagagt cgacctgcag gtccccgggg atcggtcttg ccttgctcgt cggtgatgta
     1981 cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt gcttggcaat
     2041 cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc ctcgggcgga
     2101 ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg
     2161 cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca
     2221 gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat
     2281 agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag gccgacaggc
     2341 tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca
     2401 ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct
     2461 catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg
     2521 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     2581 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     2641 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     2701 cgaagcaggg ttatgcagcg gaaaagatcc gtcgacctgc aggcatgcaa gctctagcga
     2761 ttccagacgt cccgaaggcg tggcgcggct tccccgtgcc ggagcaatcg ccctgggtgg
     2821 gttacacgac gcccctctat ggcccgtact gacggacaca ccgaagcccc ggcggcaacc
     2881 ctcagcggat gccccggggc ttcacgtttt cccaggtcag aagcggtttt cgggagtagt
     2941 gccccaactg gggtaacctt tgagttctct cagttggggg cgtagggtcg ccgacatgac
     3001 acaaggggtt gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc agtcgcgcga
     3061 gcgcgaaaat tcgagcgcag caagcccagc gacacagcgt agcgccaacg aagacaaggc
     3121 ggccgacctt cagcgcgaag tcgagcgcga cgggggccgg ttcaggttcg tcgggcattt
     3181 cagcgaagcg ccgggcacgt cggcgttcgg gacggcggag cgcccggagt tcgaacgcat
     3241 cctgaacgaa tgccgcgccg ggcggctcaa catgatcatt gtctatgacg tgtcgcgctt
     3301 ctcgcgcctg aaggtcatgg acgcgattcc gattgtctcg gaattgctcg ccctgggcgt
     3361 gacgattgtt tccactcagg aaggcgtctt ccggcaggga aacgtcatgg acctgattca
     3421 cctgattatg cggctcgacg cgtcgcacaa agaatcttcg ctgaagtcgg cgaagattct
     3481 cgacacgaag aaccttcagc gcgaattggg cgggtacgtc ggcgggaagg cgccttacgg
     3541 cttcgagctt gtttcggaga cgaaggagat cacgcgcaac ggccgaatgg tcaatgtcgt
     3601 catcaacaag cttgcgcact cgaccactcc ccttaccgga cccttcgagt tcgagcccga
     3661 cgtaatccgg tggtggtggc gtgagatcaa gacgcacaaa caccttccct tcaagccggg
     3721 cagtcaagcc gccattcacc cgggcagcat cacggggctt tgtaagcgca tggacgctga
     3781 cgccgtgccg acccggggcg agacgattgg gaagaagacc gcttcaagcg cctgggaccc
     3841 ggcaaccgtt atgcgaatcc ttcgggaccc gcgtattgcg ggcttcgccg ctgaggtgat
     3901 ctacaagaag aagccggacg gcacgccgac cacgaagatt gagggttacc gcattcagcg
     3961 cgacccgatc acgctccggc cggtcgagct tgattgcgga ccgatcatcg agcccgctga
     4021 gtggtatgag cttcaggcgt ggttggacgg cagggggcgc ggcaaggggc tttcccgggg
     4081 gcaagccatt ctgtccgcca tggacaagct gtactgcgag tgtggcgccg tcatgacttc
     4141 gaagcgcggg gaagaatcga tcaaggactc ttaccgctgc cgtcgccgga aggtggtcga
     4201 cccgtccgca cctgggcagc acgaaggcac gtgcaacgtc agcatggcgg cactcgacaa
     4261 gttcgttgcg gaacgcatct tcaacaagat caggcacgcc gaaggcgacg aagagacgtt
     4321 ggcgcttctg tgggaagccg cccgacgctt cggcaagctc actgaggcgc ctgagaagag
     4381 cggcgaacgg gcgaaccttg ttgcggagcg cgccgacgcc ctgaacgccc ttgaagagct
     4441 gtacgaagac cgcgcggcag gcgcgtacga cggacccgtt ggcaggaagc acttccggaa
     4501 gcaacaggca gcgctgacgc tccggcagca aggggcggaa gagcggcttg ccgaacttga
     4561 agccgccgaa gccccgaagc ttccccttga ccaatggttc cccgaagacg ccgacgctga
     4621 cccgaccggc cctaagtcgt ggtgggggcg cgcgtcagta gacgacaagc gcgtgttcgt
     4681 cgggctcttc gtagacaaga tcgttgtcac gaagtcgact acgggcaggg ggcagggaac
     4741 gcccatcgag aagcgcgctt cgatcacgtg ggcgaagccg ccgaccgacg acgacgaaga
     4801 cgacgcccag gacggcacgg aagacgtagc ggcgtagcga gacacccggg aagcctgatc
     4861 tacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     4921 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     4981 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     5041 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaatttatgc ggtgtgaaat
     5101 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     5161 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5221 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5281 taaaacgacg gccagtgcca agcttgggct gcaggtcgac tctagaggat ctgcagccaa
     5341 gcttgcatgc cggtctgacc gggaacgcgt cctgcaccct ggtccggtgg acaagcgcca
     5401 tcggaaccgg caatgcggtt tgttcgatcg agttggcatc ggacgcagaa ttgatcaaaa
     5461 ctactgcttc gggcatgggt cccccccagg aatcatgtga tgccgagctg ttctgtatgc
     5521 gcgaacgtta agatacagac tgagcggttt tttttctatc cttcccgggg gagacatgaa
     5581 caaggaggca ggcatggcca agcaggaccg ggcgatccgc acgcggcaga cgatcctgga
     5641 cgccgcggcg caggtcttcg agaagcaggg ctaccaagct gccacgatca cggagatcct
     5701 caaggtggcc ggggtgacca agggagccct ctacttccac ttccagtcca aggaagaact
     5761 ggcgctgggc gtcttcgacg cccaggaacc accacaggcc gttccggagc aacccctccg
     5821 gctgcaagaa ctcatcgaca tgggcatgtt gttctgtcac cgcttgcgca cgaacgtcgt
     5881 ggcccgggcc ggcgtgcgcc tctccatgga ccagcaggcg cacggtctcg atcgccgagg
     5941 acccttccgt cgctggcacg agacactcct gaagctgctg aaccaggcca aggagaacgg
     6001 tgagttgctg ccccatgtgg tcaccaccga ctcggccgat ctctacgtgg gcacgttcgc
     6061 cgggatacag gtcgtgtccc agacggtcag cgactaccag gacctcgaac accgctacgc
     6121 gctgctgcag aagcacatcc tgcccgccat cgcggttccc tccgtgctgg ccgcgctcga
     6181 tctctccgag gagcgcggag cacgcctcgc ggccgaactg gcaccgaccg ggaaggactg
     6241 accgccgaag cgcccgcacc ggataccgac ccgccgtgcc cgagcggccg accggggccg
     6301 cctacgggcc cggcggcggg cccgtaggtc tgccctgcgt accgaagcgt ggcgggtcag
     6361 agaatcgttc cgcctgtggc atcgacgtac tggccggtga tccaccgtga gtcgtcggag
     6421 gccagaaagg ccaccacgtc ggcgatgtcg tcgggtctgc cgatgcggtt gaacacggag
     6481 taggcggcca gtgccgcggc cgcctcgggg gtctgccgcc gccgtgcgtt catgtccgtc
     6541 tccacgaaac ccggcgccac cgcgttgact ctagaggatc cccgggtacc gagctcctgc
     6601 ggagcgtgct gtgtgtgttc acattcgaac ggtctctgct ttgacaaacc ggtgtgctgg
     6661 tttgtaaagt cgtggccagg agaatacgac agcgtgcagg actgggggag ttatgcgctt
     6721 tcggatggag ctcgaattca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6781 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgt
     6841 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6901 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6961 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7021 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7081 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7141 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7201 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7261 caaggcgcgt atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7321 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7381 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7441 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7501 cgccttctat cgccttcttg acgagttctt ctgagaattc cccagatccg cggccgcgcg
     7561 cgatatcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7681 gagctaactc acattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     7741 cggatcaatt cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
     7801 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
     7861 tgacccgcag ttgcaaaccc tcactgatcc gtaatgtgag ttagctcact cattaggcac
     7921 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7981 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtggcgt acaccgtcgc
     8041 ctcggtcggc ccgtagagat tggcgatccc gaccgcagca ccaccgagaa cgtccccgac
     8101 gtggccgacc agcccgtcat cgtcaacgcc tgatccgcgg tgcggacagg ccgtgtcgcg
     8161 accggccgtg cggaattaag ccggcccgta ccctgtgaat agaggtccgc tgtgacacaa
     8221 gaatccctgt tacttctcga ccgtattgat tcggatgatt cctacgcgag cctgcggaac
     8281 gaccaggaat tctgggagcc gctggcccgc cgagccctgg aggagctcgg gctgccggtg
     8341 ccgccggtgc tgcgggtgcc cggcgagagc accaaccccg tactggtcgg cgagcccggc
     8401 ccggtgatca agctgttcgg cgagcactgg tgcggtccgg agagcctcgc gtcggagtcg
     8461 gaggcgtacg cggtcctggc ggacgccccg gtgccggtgc cccgcctcct cggccgcggc
     8521 gagctgcggc ccggcaccgg agcctggccg tggccctacc tggtgatgag ccggatgacc
     8581 ggcaccacct ggcggtccgc gatggacggc acgaccgacc ggaacgcgct gctcgccctg
     8641 gcccgcgaac tcggccgggt gctcggccgg ctgcacaggg tgccgctgac cgggaacacc
     8701 gtgctcaccc cccattccga ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc
     8761 gtcgaggacc accgcgggtg gggctacctc tcgccccggc tgctggaccg cctggaggac
     8821 tggctgccgg acgtggacac gctgctggcc ggccgcgaac cccggttcgt ccacggcgac
     8881 ctgcacggga ccaacatctt cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac
     8941 ttcaccgacg tctatgcggg agactcccgc tacagcctgg tgcaactgca tctcaacgcc
     9001 ttccggggcg accgcgagat cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc
     9061 gaggacttcg cccgcgaact gctcgccttc accttcctgc acgacttcga ggtgttcgag
     9121 gagaccccgc tggatctctc cggcttcacc gatccggagg aactggcgca gttcctctgg
     9181 gggccgccgg acaccgcccc cggcgcctga cgccccgggc cgcccggcgc cgcccccggc
     9241 ccccggcggc cgccgg
//
