LOCUS       sequence-350018-       10363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3187
FEATURES             Location/Qualifiers
     source          1..10363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3117..3141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3149..3190
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3301..3889
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3354..3374)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3772..3783)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3961..4194
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4272..4393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4309..4328)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4363..4382
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4433..4568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4588..4607)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4589..4607)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4617..4634)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4617..4633)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4626..4648)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4775..5230
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4862..4881)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5072..5093
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5256..5360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5361..5429,5430..6221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5579..5598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6392..6980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6881..6900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7134..7151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7268..7289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7304..7309,7310..7327,7328..7334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7342..7358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7347..7369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7366..7382
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7366..7382
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7401..7421
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7403..7421
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7449..7675
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7676..7856
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7903..8028
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8521..8754
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8939..8983
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9214..9424
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9236..9432
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9283..9418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9464..10066
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9464..9483)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9960..9980
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10109..10226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgttcta cgcacatttt gttctcagta aaagagggcc
     1261 tctggccaaa atttggctag cggcccattg ggataagaag ctaaccaaag cccatgtgtt
     1321 cgagtgtaat ttagagagca gcgtggagag tatcatctca ccaaaggtga aaatggcatt
     1381 acggacatca ggacatctct tactgggagt agttcgaatc tatcacagga aagccaaata
     1441 ccttcttgca gactgtaatg aagcattcat taagataaag atggcttttc ggccaggtgt
     1501 ggttgacctg cctgaggaaa atcgggaagc agcttataat gccattactt tacctgaaga
     1561 atttcatgac tttgatcagc cactgcctga cttagatgac atcgatgtgg cccagcagtt
     1621 cagcttgaat cagagtagag tggaagagat aaccatgaga gaagaagttg ggaacatcag
     1681 tattttacaa gaaaatgatt ttggtgattt tggaatggat gatcgtgaga taatgagaga
     1741 aggcagtgct tttgaggatg acgacatgtt agtaagcact actacttcta acctcctatt
     1801 agagtctgaa cagagcacca gcaatctgaa tgagaaaatt aaccatttag aatatgaaga
     1861 tcaatataag gatgataatt ttggagaagg aaatgatggt ggaatattag atgacaaact
     1921 tattagtaat aatgatggcg gtatctttga tgatccccct gccctctctg aggcaggggt
     1981 gatgttgcca gagcagcctg cacatgacga tatggatgag gatgataatg tatcaatggg
     2041 tgggcctgat agtcctgatt cagtggatcc cgttgaacca atgccaacca tgactgatca
     2101 aacaacactt gttccaaatg aggaagaagc atttgcattg gagcctattg atataactgt
     2161 taaagaaaca aaagccaaga ggaagaggaa gctaattgtt gacagtgtca aagagttgga
     2221 tagcaagaca attagagccc aacttagtga ttattcagat attgttacta ctttggatct
     2281 ggcaccgccc accaagaaat tgatgatgtg gaaagagaca ggaggagtag aaaaactgtt
     2341 ttctttacct gctcagcctt tgtggaataa cagactactg aagctcttta cacgctgtct
     2401 tacaccgctt gtaccagaag accttagaaa aaggaggaaa ggaggagagg cagataattt
     2461 ggatgaattc ctcaaagaat ttgaaaatcc agaggttcct agagaggacc agcaacagca
     2521 gcatcagcag cgtgatgtta tcgatgagcc cattattgaa gagccaagcc gcctccagga
     2581 gtcagtgatg gaggccagca gaacaaacat agatgagtca gctatgcctc caccaccacc
     2641 tcagggagtt aagcgaaaag ctggacaaat tgacccagag cctgtgatgc ctcctcagca
     2701 ggtagagcag atggaaatac cacctgtaga gcttccccca gaagaacctc caaatatctg
     2761 tcagctaata ccagagttag aacttctgcc agaaaaagag aaggagaaag agaaggaaaa
     2821 agaagatgat gaagaggaag aggatgaaga tgcatcaggg ggcgatcaag atcaggaaga
     2881 aagaagatgg aacaaaagga ctcagcagat gcttcatggt cttcagcgtg ctcttgctaa
     2941 aactggagct gaatctatca gtttgcttga gttatgtcga aatacgaaca gaaaacaagc
     3001 tgccgcaaag ttctacagct tcttggttct taaaaagcag caagctattg agctgacaca
     3061 ggaagaaccg tacagtgaca tcatcgcaac acctggacca aggttccata ttatataccc
     3121 aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     3181 cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     3241 tatatcttgt ggaaaggacg attcttacac ataacaatgg acaatacgcg ttaagtcgac
     3301 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3361 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3421 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3481 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3541 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3601 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3661 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3721 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3781 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3841 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     3901 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3961 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     4201 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4261 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4321 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4381 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4501 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4561 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4621 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4681 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4741 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4801 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4861 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4921 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4981 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5041 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5101 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5161 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5221 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6061 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7141 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7201 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7261 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7321 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7381 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7441 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7501 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7561 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7621 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7681 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7741 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7801 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7861 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7921 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7981 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8041 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8101 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8161 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8221 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8281 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8341 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8401 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8461 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8521 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8581 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8641 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8701 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8761 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8821 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8881 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8941 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9001 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9061 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9121 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     9181 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     9241 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9421 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     9481 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     9541 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     9601 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9661 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9721 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9781 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9841 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     9901 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     9961 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    10021 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    10081 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10141 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10201 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    10261 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    10321 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
