LOCUS       sequence-350021-        4659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO1B3_STOP
FEATURES             Location/Qualifiers
     source          1..4659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2216..2315)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2332..2351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2333..2351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2356..2372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2356..2372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2474..2493)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2485..3294
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2552..2571)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3441..4029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3930..3949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4183..4200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4359..4386)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4478..4564)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4613..4635
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4627..4644
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4628..4644
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ccagcaccag
      121 cacctgaaca agacagccga gtccgccagc tccgagaaga agaagacccg gagatgcaat
      181 ggctttaaga tgttcctggc cgccctgtcc ttctcttaca tcgccaaggc cctgggcggc
      241 atcatcatga agatctccat cacccagatc gagaggcgct tcgacatctc tagctccctg
      301 gccggcctga tcgatggcag ctttgagatc ggcaacctgc tggtcatcgt gttcgtgagc
      361 tacttcggca gcaagctgca ccggcctaag ctgatcggca tcggctgtct gctgatgggc
      421 acaggctcca tcctgacctc tctgcctcac ttctttatgg gctactatag atatagcaag
      481 gagacacaca tcaacccatc tgagaatagc acatctagcc tgtccacctg cctgatcaac
      541 cagacactgt ccttcaatgg cacctctcca gagatcgtgg agaaggactg cgtgaaggag
      601 agcggctccc acatgtggat ctacgtgttc atgggcaaca tgctgagggg aatcggagag
      661 accccaatcg tgcctctggg catctcttac atcgacgatt ttgccaagga gggccactcc
      721 tctctgtatc tgggctccct gaatgccatc ggcatgatcg gacccgtgat cggattcgca
      781 ctgggaagcc tgtttgccaa gatgtacgtg gacatcggct atgtggatct gtccacaatc
      841 aggatcaccc caaaggattc tagatgggtg ggagcatggt ggctgggctt cctggtgagc
      901 ggcctgtttt ccatcatcag ctccatcccc ttctttttcc tgccaaagaa ccccaataag
      961 cctcagaagg agaggaagat ctctctgagc ctgcacgtgc tgaagacaaa cgacgatcgc
     1021 aatcagacag ccaacctgac caatcagggc aagaacgtga caaagaatgt gaccggcttt
     1081 ttccagtccc tgaagtctat cctgacaaac ccactgtacg tgatcttcct gctgctgacc
     1141 ctgctgcagg tgtctagctt tatcggcagc ttcacctacg tgtttaagta tatggagcag
     1201 cagtatggcc agtccgcctc tcacgccaat ttcctgctgg gcatcatcac catccccaca
     1261 gtggccaccg gcatgttcct gggcggcttt atcatcaaga agtttaagct gtctctggtg
     1321 ggcatcgcca agtttagctt cctgacaagc atgatctcct ttctgttcca gctgctgtac
     1381 ttccctctga tctgcgagag caagtccgtg gcaggactga ccctgacata cgacggcaac
     1441 aattctgtgg ccagccacgt ggatgtgcca ctgtcttatt gcaacagcga gtgcaattgt
     1501 gacgagtccc agtgggagcc cgtgtgcgga aacaatggca tcacctatct gtctccctgc
     1561 ctggccggct gtaagtcctc tagcggcatc aagaagcaca cagtgtttta caactgctct
     1621 tgcgtggagg tgaccggcct gcagaaccgg aattatagcg cccacctggg agagtgccct
     1681 agggataaca cctgtacaag aaagtttttc atctacgtgg ccatccaggt catcaactcc
     1741 ctgttctctg ccacaggcgg caccaccttc atcctgctga ccgtgaagat cgtgcagcca
     1801 gagctgaagg ccctggcaat gggcttccag tccatggtca tcaggaccct gggaggaatc
     1861 ctggcaccta tctactttgg cgccctgatc gataagacat gcatgaagtg gagcaccaac
     1921 tcctgcggag cacagggagc ctgtcggatc tacaatagcg tgtttttcgg cagagtgtat
     1981 ctgggactgt ccatcgccct gaggttcccc gccctggtgc tgtatatcgt gtttatcttc
     2041 gccatgaaga agaagtttca gggcaaggac accaaggcca gcgataacga gcgcaaagtg
     2101 atggacgagg ccaatctgga gttcctgaac aatggcgagc actttgtgcc tagcgccggc
     2161 acagattcca agacctgtaa cctggacatg caggataacg ccgccgccaa ttaagaccca
     2221 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2281 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2341 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2401 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2461 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2521 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2581 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2641 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2701 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2761 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2821 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2881 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2941 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3001 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3061 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3121 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3181 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3241 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3301 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3361 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4321 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4381 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4441 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4501 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4561 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4621 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
