LOCUS       sequence-350024-       10153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3188
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2907..2931)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2939..2980
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3091..3679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3144..3164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3562..3573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3751..3984
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4062..4183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4099..4118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4153..4172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4223..4358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4378..4397)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4379..4397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4407..4424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4407..4423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4416..4438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4565..5020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4652..4671)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4862..4883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5046..5150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5151..5219,5220..6011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5369..5388)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6182..6770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6671..6690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6924..6941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7058..7079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7094..7099,7100..7117,7118..7124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7132..7148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7137..7159
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7156..7172
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7156..7172
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7191..7211
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7193..7211
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7239..7465
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7466..7646
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7693..7818
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8311..8544
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8729..8773
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9004..9214
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9026..9222
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9073..9208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9254..9856
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9254..9273)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9750..9770
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9899..10016
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaattc cagcttgacc gcccagaggc gcggcagtga
     1261 cgccgagttg ggaccctggg tgatggctgc gaggtccaag gacgcggcgc cgtcccaacg
     1321 cgacggactt ttgcccgtga aagtggagga agactcaccc ggaagttggg agcccaacta
     1381 tcccgcggct tcgccggacc ccgaaacttc tcgactgcac tttaggcagc tgcgttacca
     1441 ggaggtggct ggaccggaag aggcgctgag ccggctccga gaactctgtc gtcggtggct
     1501 gagacccgag ctgctctcca aggagcagat cctggagctg ctggtgctgg agcagttcct
     1561 caccatcctg cccgaggagc ttcaagcctg ggtgcgagag cactgcccag agagcgggga
     1621 ggaggcggtg gccgtggtgc gggctctgca gcgagcgctc gatggaacct catcccaggg
     1681 gatggtgact ttcgaggaca cggctgtgtc tctaacctgg gaggagtggg agcgcctgga
     1741 cccagcacgg agggacttct gcagagagag tgcgcagaag gattccggga gcacagttcc
     1801 gccgagtttg gaaagcagag tggagaacaa agagttgatt ccaatgcaac aaattttaga
     1861 agaagcggag ccacaggggc aactacaaga agcgttccag gggaagcgcc ccctgttttc
     1921 taagtgtggc agtacccatg aggacagggt ggaaaagcag tccggagacc ccttgcccct
     1981 gaaacttgaa aattctcctg aagcagaagg actcaacagc atctcagatg tcaataagaa
     2041 tggttccata gaaggggaag actctaaaaa taatgaattg cagaacagtg ccaggtgttc
     2101 caaccttgtt ctatgtcagc acatcccgaa agcagagagg cccactgaca gtgaggaaca
     2161 cgggaacaag tgcaagcaaa gtttccacat ggtgacgtgg cacgtgctga aacctcacaa
     2221 gtctgacagt ggagacagtt tccatcattc cagccttttt gagacccaga ggcagctcca
     2281 tgaagaaaga ccttataaat gtggtaactg tgggaagagt ttcaaacaac gctctgacct
     2341 ctttagacac cagagaatcc acacaggtga gaaaccctat ggctgccaag aatgtgggaa
     2401 aagcttcagc cagagtgctg ccctgaccaa gcaccagagg acacacacag gcgagaagcc
     2461 gtacacctgt ctgaaatgtg gggagcgctt caggcagaat tcacacctaa atcgtcatca
     2521 aagtacccac agtagagaca aacattttaa atgtgaggaa tgcggggaaa cctgtcatat
     2581 ttccaacctt tttagacatc agagactaca taaaggggaa agaccctata agtgtgaaga
     2641 atgcgagaag agcttcaaac agcgctctga cctctttaaa caccacagaa tccacactgg
     2701 ggagaagccc tatggatgtt ccgtctgtgg gaaacgcttc aatcagagtg caaccctcat
     2761 taaacaccag agaattcaca ctggggaaaa gccttacaaa tgtcttgaat gtggggaaag
     2821 atttagacaa agtacacacc ttatccgaca ccaaagaatt catcaaaata aagtgctgtc
     2881 ggctgggcgt ggtggctcac gcctataccc aactttcttg tacaaagtgg ttgatatcgg
     2941 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta
     3001 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg acgcggcatg
     3061 gcaagagttc atatgacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3121 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3181 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3241 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3301 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3361 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3421 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3481 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3541 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3601 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3661 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3721 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3781 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3841 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3901 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3961 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     4021 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4201 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4261 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4321 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4381 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4441 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4501 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4561 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4621 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4681 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4741 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4801 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4861 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4921 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4981 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5041 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5101 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5161 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5221 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5281 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5341 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5401 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5461 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5521 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5581 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5641 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5701 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5761 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5821 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5881 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5941 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6001 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6061 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6841 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6901 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6961 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7021 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7081 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7141 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7201 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7261 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7321 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7381 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7441 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7501 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7561 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7621 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7681 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7741 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7801 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7861 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7921 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7981 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8041 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8101 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8161 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8221 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8281 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8341 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8401 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8461 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8521 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8581 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8641 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8701 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8761 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8821 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8881 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8941 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9001 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     9061 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9121 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9181 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     9241 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9301 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9361 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9421 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9481 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9541 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9601 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9661 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9721 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9781 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9841 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9901 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9961 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10021 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    10081 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    10141 aggagtggga att
//
