LOCUS       sequence-350027-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3189
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2430..2454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2462..2503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2614..3202
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2667..2687)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3085..3096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3274..3507
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3585..3706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3622..3641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3676..3695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3746..3881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3901..3920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3902..3920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3930..3947)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3930..3946)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3939..3961)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4088..4543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4175..4194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4385..4406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4569..4673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4674..4742,4743..5534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4892..4911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5705..6293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6194..6213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6447..6464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6617..6622,6623..6640,6641..6647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6660..6682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6679..6695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6679..6695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6714..6734
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6716..6734
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6762..6988
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6989..7169
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7216..7341
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7834..8067
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8252..8296
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8527..8737
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8549..8745
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8596..8731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8777..9379
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8777..8796)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9273..9293
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9422..9539
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcggc tgtcatcctg ccctcgactg ctgctccgtc
     1261 ttccctgttc ccagcctctc agcaaaaagg acacacacag ggcggagagc tggttaatga
     1321 gctcctgaca agctggctac ggggcttggt aaccttcgag gatgtggccg tggagttcac
     1381 ccaggaggag tgggcgttgc tggaccctgc ccaaaggaca ctgtacaggg atgtgatgct
     1441 ggagaactgc aggaacctgg cctcactagg gtgtcgtgtt aataaaccca gtctgatatc
     1501 ccagttggaa caagacaaga aggtggtgac agaggaaaga ggaattctac caagcacctg
     1561 tccagatttg gagactctac ttaaagccaa atggttaact cctaagaaga atgttttcag
     1621 aaaagaacag tctaaaggtg taaaaacgga aagaagtcat cgtggagtga aactcaatga
     1681 atgtaatcag tgttttaaag tcttcagcac gaaatctaac ctaactcagc acaagagaat
     1741 tcatactgga gaaaaaccct atgactgtag tcaatgtggg aagtccttca gtagcagatc
     1801 ttaccttact attcataaga gaatccataa tggggagaaa ccctatgaat gcaatcactg
     1861 tgggaaagca tttagtgatc cctcatccct tagactgcat ttgagaattc acactggaga
     1921 aaaaccctat gaatgtaacc agtgttttca cgttttccgc accagttgta acctcaaaag
     1981 ccacaagagg attcacacgg gggagaatca ccatgaatgt aatcagtgtg gaaaagcttt
     2041 cagcacaagg tcctctctca ctgggcacaa tagcattcat acaggggaga aaccttatga
     2101 atgtcacgat tgtgggaaaa ccttcaggaa gagctcctat ctgacacagc acgtaagaac
     2161 tcatactgga gaaaaaccct atgaatgtaa cgagtgtggg aaatccttca gcagtagctt
     2221 ttctcttact gtgcacaaga gaatacatac cggagagaaa ccctacgagt gcagtgactg
     2281 tggaaaagcc tttaataatc tctcagctgt gaagaaacac ttaagaactc acactggaga
     2341 aaaaccctat gaatgtaatc attgtgggaa atccttcaca agtaactcct atctttctgt
     2401 gcacaagaga atacataata gatggatatg cccaactttc ttgtacaaag tggttgatat
     2461 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc
     2521 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatatcgt
     2581 ggtacgagga aacttgatac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3001 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3121 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3181 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3241 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3301 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3361 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3421 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3481 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3541 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3721 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3901 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3961 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4021 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4081 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4141 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4381 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4441 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4501 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6361 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6421 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6481 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6541 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6601 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6661 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6721 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6781 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6841 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6901 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6961 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7021 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7081 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7141 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7201 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7261 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7321 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7381 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7441 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7501 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7561 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7621 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7681 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7741 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7801 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7861 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7921 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7981 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8041 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8101 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8161 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8221 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8281 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8341 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8401 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8461 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8521 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8581 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8641 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8701 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     8761 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8821 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8881 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8941 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9001 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9061 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9121 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9181 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9241 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9301 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9361 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9421 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9481 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9541 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
     9601 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9661 gaaaggagtg ggaatt
//
