LOCUS       sequence-350033-       11157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (pFRO6::SMT2-FLAG::tOCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, 
            Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, 
            Gallart-Ayala H, Ivanisevic J, Fankhauser C
  TITLE     A combination of plasma membrane sterol biosynthesis and autophagy 
            is required for shade-induced hypocotyl elongation.
  JOURNAL   Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9.
  PUBMED    36216814
REFERENCE   2  (bases 1 to 11157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-10"; volume: "13(1):5659. doi"; pages: " 
            10.1038/s41467-022-33384-9"
COMMENT     Sequence Label: pYI047
FEATURES             Location/Qualifiers
     source          1..11157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2262..2285
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2294..3001
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(join(3244..3954,3955..3957,3958..3960))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3244..3954,3955..3957,3958..3960))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3286..3307)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3635..3654
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3894..3915
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4596..4620
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5141..5932
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6178..6766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6667..6686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6920..6937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6952..7092
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7018..7037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7178..7200
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7436..7630
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7696..8769)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9198..9827)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagctcgtg ctctcaaggc caaagaacat tatggaaatt
      121 cgaagatata ttatcgatgc atataattta tataatcaca gtctgtatgt attatggaaa
      181 caactttaac taatttttga gattgatgct acttcatgat agcaatttct tagttctttt
      241 gagttaataa cttaatatat aaatataatg gtacaacaac cattacaatc gtcattataa
      301 tcatcttatc catactcaat agtcaatact ctcaccttcc ctaaagtgtg gttgaaatca
      361 aactttactc tctctaggcc tgattataat tcttttttgt ctacacatac aacttcatga
      421 gacttaatta taatttctta gtgttctttt gttgacatgt aaatcgtagc taagtctaag
      481 tggtactttt atttgttatt ggattttgtt tcggttactt aaaagtgtaa tgaagaattt
      541 ttttgactat atacatctaa gtttttcctt ggactgatta taacttgtgt gttcgagatt
      601 gatacaactt catgatagtc gattattctt gtttaacgta acacattgtt agatagtgtc
      661 atttaccaga aacaatctaa cagtgaaact ttgcaaattt atctctctgc tggatcttga
      721 acgcggaaac cgaataatgt ttctttcgag ctaatccgaa tgatgttgaa attctatatg
      781 ttcagattca ggtaatgtaa gcttatccaa taaccatcat agtcgtcata ataaccatct
      841 tatctatact aaagtagtaa gtactctcag tctctcacct tacccttaat tgtggttgaa
      901 atcaacctaa cgtgacactc tctcccaagt acgttactct cacgtgttaa tgacactctc
      961 attttttttt atagtttttt ttattgtgta ctaggattgt tgtccgaagg aatctttcat
     1021 aaatgagaat gatagaaacc tcaatggtcc agaaaatttt acttgactct tctaagaata
     1081 tcttattagt ttatctctag taacgccacc ttaaaagccg atgctgcttc tgagccaact
     1141 tctcatcaca acactgagaa gtggaaattc aaataaagat ggactcttta acactcttct
     1201 tcaccggtgc actcgtcgcc gtcggtatct actggttcct ctgcgttctc ggtccagcag
     1261 agcgtaaagg caaacgagcc gtagatctct ctggtggctc aatctccgcc gagaaagtcc
     1321 aagacaacta caaacagtac tggtctttct tccgccgtcc aaaagaaatc gaaaccgccg
     1381 agaaagttcc agacttcgtc gacacattct acaatctcgt caccgacata tacgagtggg
     1441 gatggggaca atccttccac ttctcaccat caatccccgg aaaatctcac aaagacgcca
     1501 cgcgcctcca cgaagagatg gccgtagatc tgatccaagt caaacctggt caaaagatcc
     1561 tagacgtcgg atgcggtgtc ggcggtccga tgcgagcgat tgcatctcac tcgcgagcta
     1621 acgtagtcgg gattacaata aacgagtatc aggtgaacag agctcgtctc cacaataaga
     1681 aagctggtct cgacgcgctt tgcgaggtcg tgtgtggtaa cttcctccag atgccgttcg
     1741 atgacaacag tttcgacggt gcttattcca tcgaagccac gtgtcacgcg ccgaagctgg
     1801 aagaagtgta cgcagagatc tacagggtgt tgaaacccgg atctatgtat gtgtcgtacg
     1861 agtgggttac gacggagaaa tttaaggcgg aggatgacga acacgtggag gtaatccaag
     1921 ggattgagag aggcgatgcg ttaccagggc ttagggctta cgtggatata gctgagacgg
     1981 ctaaaaaggt tgggtttgag atagtgaagg agaaggatct ggcgagtcca ccggctgagc
     2041 cgtggtggac taggcttaag atgggtaggc ttgcttattg gaggaatcac attgtggttc
     2101 agattttgtc agcggttgga gttgctccta aaggaactgt tgatgttcat gagatgttgt
     2161 ttaagactgc tgattatttg accagaggag gtgaaaccgg aatattctct ccgatgcata
     2221 tgattctctg cagaaaaccg gagtcaccgg aggagagttc tgattacaag gacgacgatg
     2281 acaagtgagg atcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc
     2341 tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg
     2401 ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat
     2461 attctccgtt caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa
     2521 acaatatatt gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa
     2581 acaattgcgt tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc
     2641 taaaagactg attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct
     2701 acgacacacc gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg
     2761 cgcgcatggg tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg
     2821 ggcaccattc aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt
     2881 atgcagcatt tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt
     2941 gacgacaaat cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca
     3001 ggacctctag agcgccactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     3061 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     3121 ctataagaac cctaattccc ttatctggga actactcaca cattattata gagagagata
     3181 gatttgtaga gagagactgg tgatttcagc gatgcagccg ggcggcgctt acagctcgtc
     3241 cttcttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     3301 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     3361 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     3421 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     3481 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     3541 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     3601 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     3661 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     3721 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     3781 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     3841 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     3901 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     3961 gtcggccgag gataatgata ggagaagtga aaagatgaga aagagaaaaa gattagtctt
     4021 caccatggct atcgttcgta aatggtgaaa attttcagaa aattgctttt gctttaaaag
     4081 aaatgattta aattgctgca atagaagtag aatgcttgat tgcttgagat tcgtttgttt
     4141 tgtatatgtt gtgttgagaa ttcgcccttg ttttgctatt tgtgtatgtt ttcttgtttt
     4201 ggtttgagtg aagagtgtag tgaagggttg gtttgtattt ataggtgaga aaggagattt
     4261 gcatggcaat cacgtgtaag aatgcatgca tgcatgagtg tgtgtggaga gagagagatg
     4321 ctcttacgtt gttgtcggga ttatgtgttg gaaaagagtg acaagtgacg aacaaaacaa
     4381 taccactcat catagctccg cttatgcatg ctatgtgtga tttgtagctc ttgttctgac
     4441 atgacactat gaacaatttt ttaaaaagaa ggttaaactt ttatttaaat aaaaacccta
     4501 tgttaatttg gtttcaaggg cgaattcaat tcggcgttaa ttcagtacat taaaaacgtc
     4561 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     4621 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     4681 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     4741 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     4801 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     4861 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     4921 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     4981 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     5041 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     5101 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     5161 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     5221 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     5281 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     5341 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     5401 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     5461 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     5521 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     5581 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     5641 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     5701 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     5761 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     5821 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     5881 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     5941 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     6001 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     6061 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     6121 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6181 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6241 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6301 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6361 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6421 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6541 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6781 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6841 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6901 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6961 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     7021 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     7081 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7141 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7201 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     7261 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     7321 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     7381 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     7441 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     7501 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     7561 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     7621 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     7681 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     7741 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     7801 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     7861 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     7921 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     7981 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     8041 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     8101 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     8161 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     8221 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     8281 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     8341 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     8401 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     8461 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     8521 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     8581 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     8641 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     8701 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     8761 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
     8821 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     8881 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     8941 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     9001 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     9061 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     9121 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     9181 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     9241 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     9301 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     9361 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     9421 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     9481 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     9541 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     9601 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     9661 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     9721 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     9781 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     9841 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     9901 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     9961 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    10021 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    10081 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    10141 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    10201 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    10261 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    10321 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    10381 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    10441 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    10501 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    10561 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    10621 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    10681 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    10741 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    10801 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    10861 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    10921 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    10981 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
    11041 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    11101 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttag
//
