LOCUS       sequence-350044-        8485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 
            ribosomal frameshifting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva 
            Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach 
            BD, Guo JU
  TITLE     Restriction of SARS-CoV-2 replication by targeting programmed -1 
            ribosomal frameshifting.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi:
            10.1073/pnas.2023051118.
  PUBMED    34185680
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-29"; volume: "118(26). pii: 
            2023051118. doi"; pages: " 10.1073/pnas.2023051118"
COMMENT     Sequence Label: pCherryGFP-CoV2-WT
FEATURES             Location/Qualifiers
     source          1..8485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    211..232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(247..252,253..270,271..277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    285..301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     290..312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     309..325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     309..325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     375..394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             586..1219
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1266..1391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1888..2121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2306..2350
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2643..2760
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2803..3201
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      3210..3219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3216..3923
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3361..3379)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3629..3648
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3924..3947
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4061..4723
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4793..4846
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4847..5446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4847..4866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5343..5363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5460..6048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5513..5533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5931..5942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6123..6356
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6475..6493)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6561..6665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6666..6734,6735..7526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6884..6903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7697..8285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8186..8205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8439..8456
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
       61 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      121 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
      181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      301 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
      361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
      421 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
      481 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
      541 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
      601 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
      661 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
      721 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
      781 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
      841 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
      901 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
      961 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     1021 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1081 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1141 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1201 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     1261 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     1321 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     1381 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     1441 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1501 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1561 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1621 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1681 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     1741 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1801 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1861 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1921 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1981 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     2041 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     2101 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     2161 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2221 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2281 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2341 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2401 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2461 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2521 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2581 tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac
     2641 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2701 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2761 cgggtttatt acagggacag cagagatcca gtttggacta gtggcctccg cgccgggttt
     2821 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     2881 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     2941 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     3001 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     3061 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     3121 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     3181 atcgctgtga tcgtcacttg gcggccgccg ccaccatggt gagcaagggc gaggaggata
     3241 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     3301 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3361 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     3421 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     3481 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     3541 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     3601 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3661 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3721 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3781 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3841 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3901 gcggcatgga cgagctgtac aaggactaca aggatgacga cgataagtcg tttttaaacg
     3961 ggtttgcggt gtaagtgcag cccgtcttac accgtgcggc acaggcacta gtactgatgt
     4021 cgtatacagg gcttttgacg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     4081 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     4141 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     4201 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     4261 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     4321 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     4381 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     4441 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     4501 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     4561 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     4621 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     4681 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     4741 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag
     4801 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     4861 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     4921 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     4981 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5041 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5101 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5161 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5221 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5281 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5341 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5401 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaatcg atagatccta
     5461 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5521 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5581 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5641 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5701 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5761 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5821 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5881 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5941 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6001 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     6061 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6121 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6181 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6241 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6301 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6361 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6421 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     6481 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6541 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6601 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6661 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6721 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6781 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6841 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6901 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6961 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7021 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7081 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7141 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7201 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7261 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7321 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7381 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7441 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7501 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7561 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7621 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7681 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7741 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7801 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7861 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7921 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7981 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8041 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8101 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8161 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8221 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8281 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8341 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8401 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8461 cggaagagcg cccaatacgc aaacc
//
