LOCUS       sequence-350045-        8438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 
            ribosomal frameshifting. No FSE was inserted. mCherry and GFP are 
            expressed equally (positive control)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva 
            Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach 
            BD, Guo JU
  TITLE     Restriction of SARS-CoV-2 replication by targeting programmed -1 
            ribosomal frameshifting.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi:
            10.1073/pnas.2023051118.
  PUBMED    34185680
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-29"; volume: "118(26). pii: 
            2023051118. doi"; pages: " 10.1073/pnas.2023051118"
COMMENT     Sequence Label: pCherryGFP-Inframe-noFSE
FEATURES             Location/Qualifiers
     source          1..8438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    238..259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(274..279,280..297,298..304)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    312..328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     317..339
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     336..352
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     336..352
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     402..421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             613..1246
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1293..1418
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1915..2148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2333..2377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2670..2787
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2830..3228
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      3237..3246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3243..3950
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3388..3406)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3656..3675
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3951..3974
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4002..4664
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4734..4787
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4788..5387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4788..4807)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5284..5304
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5440..6028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5493..5513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5911..5922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6103..6336
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6455..6473)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6541..6645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6646..6714,6715..7506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6864..6883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7677..8265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8166..8185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8419..8436
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      181 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      301 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
      361 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      421 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
      481 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
      541 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
      601 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
      661 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
      721 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
      781 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
      841 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
      901 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
      961 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1021 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     1081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1261 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1321 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1381 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1441 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1501 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1561 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1621 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1681 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1741 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1801 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1861 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1921 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1981 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     2041 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     2101 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     2161 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     2221 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2281 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2341 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2401 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2461 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2521 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2581 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg
     2641 aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2701 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2761 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2821 tggactagtg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     2881 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     2941 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     3001 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     3061 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     3121 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     3181 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggcg gccgccgcca
     3241 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     3301 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3421 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     3481 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     3541 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     3601 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     3661 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     3721 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     3781 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     3841 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3901 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag gactacaagg
     3961 atgacgacga taagaccggt gagagcgacg agagcggcct gcccgccatg gagatcgagt
     4021 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     4081 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     4141 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     4201 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     4261 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     4321 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     4381 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     4441 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     4501 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     4561 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     4621 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     4681 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca
     4741 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca
     4801 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     4861 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     4921 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     4981 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5041 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5101 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5161 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5221 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5281 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5341 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagga tccctggcgt
     5401 atcagagcag atctctatta tgtattatcg atagatccta atcaacctct ggattacaaa
     5461 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5521 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5581 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5641 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5701 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5761 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5821 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     5881 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5941 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6001 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     6061 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6121 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6301 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6361 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     6421 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     6481 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8401 agccgaacga ccgagcgcag cgagtcagtg agcgagga
//
