LOCUS       sequence-350046-        8486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 
            ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP 
            translation is prevented by stop codon within FSE (negative 
            control)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva 
            Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach 
            BD, Guo JU
  TITLE     Restriction of SARS-CoV-2 replication by targeting programmed -1 
            ribosomal frameshifting.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi:
            10.1073/pnas.2023051118.
  PUBMED    34185680
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-29"; volume: "118(26). pii: 
            2023051118. doi"; pages: " 10.1073/pnas.2023051118"
COMMENT     Sequence Label: pCherryGFP-CoV2-Inframe-stop
FEATURES             Location/Qualifiers
     source          1..8486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2130..2363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2438..3026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2544..2555
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2953..2973
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3040..3639)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3123..3143)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3620..3639
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3640..3693)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3763..4425)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             complement(4540..4563)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(4564..5271)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4839..4858)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5108..5126
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5268..5277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5286..5684)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    5727..5844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6137..6181)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6366..6599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7096..7221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7268..7901
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8093..8112)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8162..8178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8162..8178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8175..8197)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8186..8202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8210..8216,8217..8234,8235..8240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8255..8276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatg atctagccct
     2041 ttccttaatt aacccggtcc tctcactctc tgatattcat ttctttgcaa gttataaata
     2101 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     2161 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     2221 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     2281 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     2341 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     2401 agctgccttg taagtcattg gtcttaaagg atctcaggcg gggaggcggc ccaaagggag
     2461 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     2521 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     2581 cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac
     2641 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     2701 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     2761 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     2821 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     2881 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     2941 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     3001 caaattttgt aatccagagg ttgattagga tctatcgatt caggcaccgg gcttgcgggt
     3061 catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag
     3121 ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc
     3181 gcgctcggcc gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg
     3241 gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc
     3301 caggaggcct tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg
     3361 cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc
     3421 caccgcggcg ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag
     3481 gaagagttct tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg
     3541 cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc
     3601 gcgggtggcg aggcgcaccg tgggcttgta ctcggtcata gggccgggat tctcctccac
     3661 gtcaccgcat gttagaagac ttcctctgcc ctcgcgagat ccggtggagc cgggtccggc
     3721 ggtgccgtcc acggcagaat tggacgactg agcgcgggat ctggcgaagg cgatgggggt
     3781 cttgaaggcg tgctggtact ccacgatgcc cagctcggtg ttgctgtgca gctcctccac
     3841 gcggcggaag gcgaacatgg ggcccccgtt ctgcaggatg ctggggtgga tggcgctctt
     3901 gaagtgcatg tggctgtcca ccacgaagct gtagtagccg ccgtcgcgca ggctgaaggt
     3961 gcgggcgaag ctgcccacca gcacgttatc gcccatgggg tgcaggtgct ccacggtggc
     4021 gttgctgcgg atgatcttgt cggtgaagat cacgctgtcc tcggggaagc cggtgcccac
     4081 caccttgaag tcgccgatca cgcggccggc ctcgtagcgg tagctgaagc tcacgtgcag
     4141 cacgccgccg tcctcgtact tctcgatgcg ggtgttggtg tagccgccgt tgttgatggc
     4201 gtgcaggaag gggttctcgt agccgctggg gtaggtgccg aagtggtaga agccgtagcc
     4261 catcacgtgg ctcagcaggt aggggctgaa ggtcagggcg cctttggtgc tcttcatctt
     4321 gttggtcatg cggccctgct tgggggtgcc ctctccgccg cccaccagct cgaactccac
     4381 gccgttcagg gtgccggtga tgcggcactc gatctccatg gcgggcaggc cgctctcgtc
     4441 gctctcggtc aaaagccctg tatacgacat cagtactagt gcctgtgccg cacggtgtaa
     4501 gacgggctgc acttacaccg caaacccgtt taaaaacgac ttatcgtcgt catccttgta
     4561 gtccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
     4621 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
     4681 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
     4741 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     4801 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     4861 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     4921 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     4981 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     5041 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     5101 gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     5161 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     5221 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tggtggcggc
     5281 ggccgccaag tgacgatcac agcgatccac aaacaagaac cgcgacccaa atcccggctg
     5341 cgacggaact agctgtgcca cacccggcgc gtccttatat aatcatcggc gttcaccgcc
     5401 ccacggagat ccctccgcag aatcgccgag aagggactac ttttcctcgc ctgttccgct
     5461 ctctggaaag aaaaccagtg ccctagagtc acccaagtcc cgtcctaaaa tgtccttctg
     5521 ctgatactgg ggttctaagg ccgagtctta tgagcagcgg gccgctgtcc tgagcgtccg
     5581 ggcggaagga tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag
     5641 gaggggggcg cccgcgggag gcgccaaaac ccggcgcgga ggccactagt ccaaactgga
     5701 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     5761 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     5821 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtga attcggcgat
     5881 accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     5941 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     6001 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     6061 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     6121 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     6181 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     6241 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     6301 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     6361 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     6421 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     6481 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     6541 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     6601 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     6661 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     6721 tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact
     6781 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     6841 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     6901 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     6961 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     7021 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     7081 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     7141 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     7201 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     7261 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     7321 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     7381 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     7441 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     7501 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca
     7561 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa
     7621 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg
     7681 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc
     7741 tggctctact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg
     7801 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     7861 aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac
     7921 atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga
     7981 gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat
     8041 gattttggtt agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt
     8101 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa ttcgtaatca
     8161 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8221 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8281 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccacggg gagctttttg
     8341 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     8401 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catgagcttg ctgcattaat
     8461 gaatcggcca acgcgcgggg agaggc
//
