LOCUS       sequence-350052-        8719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded ratiometric fluorescent thermometer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Vu CQ, Fukushima SI, Wazawa T, Nagai T
  TITLE     A highly-sensitive genetically encoded temperature indicator 
            exploiting a temperature-responsive elastin-like polypeptide.
  JOURNAL   Sci Rep. 2021 Aug 13;11(1):16519. doi: 10.1038/s41598-021-96049-5.
  PUBMED    34389773
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-13"; volume: "11(1):16519. doi"; pages: " 
            10.1038/s41598-021-96049-5"
COMMENT     Sequence Label: ELP-TEMP/pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        126..505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        506..709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     660..680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     754..773
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        754..772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(878..880,881..883,884..1594)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3455..3457,3458..3460,3461..4171)
                     /codon_start=1
                     /product="monomeric optimized variant of ECFP (Kremers et 
                     al., 2006)"
                     /label=SCFP3A
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4201..4218)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4207..4431
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4477..4905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4564..4583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4774..4795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4914..4934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4919..5248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5099..5234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5161..5180
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5315..6109
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5369..5388)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5979..5998
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6283..6404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6320..6339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6374..6393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6453..6469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6453..6469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6466..6488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6501..6507,6508..6525,6526..6531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6684..6701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6855..7443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6935..6954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7614..8405,8406..8474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8237..8256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8475..8579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8654..8673)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
       61 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      121 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      181 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      241 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      301 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      361 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      421 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      481 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      541 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      601 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      661 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      721 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      781 agctggctag cgtttaaacg ggccctctag actcgagcgg ccgccactgt gctggatatc
      841 tgcagaattc caccacactg gactagtgga tcccaccatg gtgagcaagg gcgaggagct
      901 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      961 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     1021 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     1081 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1141 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1201 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1261 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1321 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1381 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     1441 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
     1501 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1561 cgggatcact ctcggcatgg acgagctgta caagctcgag tcttccggtg gcgttggggt
     1621 acccggggta ggtgttcccg gtgttggagt tcccggggtc ggtgtcccag gggtcggagt
     1681 gcctggcgtg ggtgtacccg gtgtcggggt tccaggtgtc ggtgtgcctg gagtaggtgt
     1741 ccctggcgtt ggcgtcccag gagtgggcgt ccctggggtt ggtgttcctg gagttggcgt
     1801 accgggggtg ggggtacctg gagtgggagt gccgggtgtt ggtgtgcccg gtgtgggtgt
     1861 ccccggagtt ggggttcctg gtgttggcgt gcctggtgtc ggcgttccgg gtgtgggggt
     1921 ccctggtgtg ggcgttcccg gcgtaggtgt accgggtgtc ggagttccgg gagtaggcgt
     1981 acctggggtc ggggtaccgg gcgttggtgt accaggagtt ggagtgccag gcgtcggagt
     2041 acccggagtc ggagtcccgg gtgtaggtgt gccgggggtt ggcgttcctg gggtgggcgt
     2101 accaggggtg ggagtacctg gtgtaggcgt gccaggtgtg ggagttcctg gcgtcggtgt
     2161 tccgggcgta ggggtgcctg gggtaggggt accaggtgtt ggggtgccgg gagtgggtgt
     2221 tccaggcgtg ggggtgcccg gggttggggt cccaggtgta ggggtccccg gggtgggtgt
     2281 gccaggagtc ggggtgccag gggttggagt accaggcgtt ggagtccctg gagtcggcgt
     2341 acccggcgtg ggagtcccag gcgtaggcgt ccccggtgta ggagtaccgg gagttggtgt
     2401 cccgggagtc ggtgtacctg gcgtaggagt tccaggggta ggcgttccag gagtaggagt
     2461 gcccggagta ggggttcccg gagtgggggt tccgggggta ggagtccccg gctcgggagt
     2521 gccggtcgag tcttccggtg gcgttggggt acccggggta ggtgttcccg gtgttggagt
     2581 tcccggggtc ggtgtcccag gggtcggagt gcctggcgtg ggtgtacccg gtgtcggggt
     2641 tccaggtgtc ggtgtgcctg gagtaggtgt ccctggcgtt ggcgtcccag gagtgggcgt
     2701 ccctggggtt ggtgttcctg gagttggcgt accgggggtg ggggtacctg gagtgggagt
     2761 gccgggtgtt ggtgtgcccg gtgtgggtgt ccccggagtt ggggttcctg gtgttggcgt
     2821 gcctggtgtc ggcgttccgg gtgtgggggt ccctggtgtg ggcgttcccg gcgtaggtgt
     2881 accgggtgtc ggagttccgg gagtaggcgt acctggggtc ggggtaccgg gcgttggtgt
     2941 accaggagtt ggagtgccag gcgtcggagt acccggagtc ggagtcccgg gtgtaggtgt
     3001 gccgggggtt ggcgttcctg gggtgggcgt accaggggtg ggagtacctg gtgtaggcgt
     3061 gccaggtgtg ggagttcctg gcgtcggtgt tccgggcgta ggggtgcctg gggtaggggt
     3121 accaggtgtt ggggtgccgg gagtgggtgt tccaggcgtg ggggtgcccg gggttggggt
     3181 cccaggtgta ggggtccccg gggtgggtgt gccaggagtc ggggtgccag gggttggagt
     3241 accaggcgtt ggagtccctg gagtcggcgt acccggcgtg ggagtcccag gcgtaggcgt
     3301 ccccggtgta ggagtaccgg gagttggtgt cccgggagtc ggtgtacctg gcgtaggagt
     3361 tccaggggta ggcgttccag gagtaggagt gcccggagta ggggttcccg gagtgggggt
     3421 tccgggggta ggagtccccg gctcgggaga attcatggtg agcaagggcg aggagctgtt
     3481 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3541 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3601 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
     3661 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3721 gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac
     3781 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3841 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acttcagcga
     3901 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     3961 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     4021 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     4081 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4141 gatcactctc ggcatggacg agctgtacaa gtaaaagctt aagtttaaac cgctgatcag
     4201 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4261 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4321 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4381 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4441 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4681 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4801 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     4921 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4981 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5041 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5161 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5221 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5281 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5341 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5401 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5461 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5521 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5581 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5641 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5701 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5761 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5821 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5881 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5941 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6001 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6061 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6121 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6181 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6241 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6301 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6361 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6421 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8581 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8641 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8701 cttgtgtgtt ggaggtcgc
//
