LOCUS       sequence-350071-        6836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S1/S2 Polybasic site deficient S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Yu S, Zheng X, Zhou B, Li J, Chen M, Deng R, Wong G, Lavillette D, 
            Meng G
  TITLE     SARS-CoV-2 spike engagement of ACE2 primes S2' site cleavage and 
            fusion initiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Jan 4;119(1). pii: 2111199119. doi: 
            10.1073/pnas.2111199119.
  PUBMED    34930824
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-01-4"; volume: "119(1). pii: 
            2111199119. doi"; pages: " 10.1073/pnas.2111199119"
COMMENT     Sequence Label: pVAX1-SARS-CoV-2 delRRAR S HA-tag
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     570..590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     664..683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4537..4563
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4660..4677)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4666..4890
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             5063..5857
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5063..5857
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5117..5136)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5727..5746
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      6183..6771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6672..6691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgccacca tgttcgtgtt tctggtgctg ctgcctctgg tgagctccca gtgcgtgaat
      781 ctgaccacaa ggacccagct gccccctgcc tataccaact ccttcacacg gggcgtgtac
      841 tatcccgaca aggtgttccg gagcagcgtg ctgcactcca cacaggatct gtttctgcct
      901 ttcttttcta acgtgacctg gttccacgcc atccacgtga gcggcaccaa tggcacaaag
      961 aggttcgaca acccagtgct gcccttcaat gatggcgtgt acttcgcctc caccgagaag
     1021 tctaatatca tccgcggctg gatctttggc accacactgg acagcaagac acagtccctg
     1081 ctgatcgtga acaatgccac caacgtggtc atcaaggtgt gcgagttcca gttttgtaac
     1141 gatccattcc tgggcgtgta ctatcacaag aacaataagt cttggatgga gagcgagttt
     1201 cgcgtgtatt cctctgccaa caattgcaca tttgagtacg tgtcccagcc cttcctgatg
     1261 gacctggagg gcaagcaggg caatttcaag aacctgcggg agttcgtgtt taagaacatc
     1321 gatggctact tcaaaatcta ctccaagcac accccaatca atctggtgag agacctgcca
     1381 cagggcttct ctgccctgga gccactggtg gatctgccca tcggcatcaa catcacccgg
     1441 tttcagacac tgctggccct gcacagaagc tacctgacac caggcgacag ctcctctgga
     1501 tggaccgcag gagcagcagc ctactatgtg ggctatctgc agcccaggac cttcctgctg
     1561 aagtacaacg agaatggcac catcacagac gcagtggatt gcgcactgga ccccctgtct
     1621 gagaccaagt gtacactgaa gagctttacc gtggagaagg gcatctatca gacaagcaac
     1681 ttcagggtgc agcctaccga gtccatcgtg cgctttccca atatcacaaa cctgtgccct
     1741 tttggcgagg tgttcaatgc aaccaggttc gcaagcgtgt acgcatggaa taggaagcgc
     1801 atctccaact gcgtggccga ctattctgtg ctgtacaata gcgcctcctt ctctaccttt
     1861 aagtgctacg gcgtgagccc cacaaagctg aatgacctgt gctttaccaa cgtgtacgcc
     1921 gattccttcg tgatcagggg cgacgaggtg cgccagatcg caccaggaca gacaggcaag
     1981 atcgcagact acaactataa gctgcctgac gatttcaccg gctgcgtgat cgcctggaac
     2041 agcaacaatc tggatagcaa agtgggcggc aactacaatt atctgtaccg gctgtttaga
     2101 aagtctaacc tgaagccatt cgagagggac atctccacag aaatctacca ggccggctct
     2161 accccctgca atggcgtgga gggctttaac tgttatttcc ctctgcagag ctacggcttc
     2221 cagccaacaa atggcgtggg ctatcagccc taccgcgtgg tggtgctgtc ttttgagctg
     2281 ctgcacgccc ctgcaacagt gtgcggacca aagaagagca ccaatctggt gaagaacaag
     2341 tgcgtgaact tcaacttcaa cggactgacc ggcacaggcg tgctgaccga gtccaacaag
     2401 aagttcctgc cttttcagca gttcggcagg gacatcgcag ataccacaga cgccgtgcgc
     2461 gaccctcaga ccctggagat cctggatatc acaccatgct ccttcggcgg cgtgtctgtg
     2521 atcacaccag gcaccaatac aagcaaccag gtggccgtgc tgtatcagga cgtgaactgt
     2581 accgaggtgc ccgtggcaat ccacgcagat cagctgaccc ctacatggcg ggtgtactct
     2641 accggcagca acgtgttcca gacaagagcc ggatgcctga tcggagcaga gcacgtgaac
     2701 aatagctatg agtgcgacat ccctatcggc gccggcatct gtgcctccta ccagacccag
     2761 acaaactccc catctgtggc aagccagtcc atcatcgcct ataccatgag cctgggcgcc
     2821 gagaactccg tggcctactc caacaattct atcgccatcc ctaccaattt cacaatctcc
     2881 gtgaccacag agatcctgcc agtgagcatg accaagacat ccgtggactg cacaatgtat
     2941 atctgtggcg attccaccga gtgctctaat ctgctgctgc agtacggctc tttttgtacc
     3001 cagctgaaca gagccctgac aggcatcgcc gtggagcagg acaagaatac acaggaggtg
     3061 ttcgcccagg tgaagcaaat ctacaagacc ccacccatca aggactttgg cggcttcaac
     3121 tttagccaga tcctgcccga tcctagcaag ccatccaagc ggtcttttat cgaggacctg
     3181 ctgttcaata aggtgaccct ggccgatgcc ggcttcatca agcagtatgg cgattgcctg
     3241 ggcgacatcg ccgccagaga cctgatctgt gcccagaagt ttaacggcct gaccgtgctg
     3301 cctccactgc tgacagatga gatgatcgcc cagtacacat ctgccctgct ggcaggcacc
     3361 atcacaagcg gatggacctt cggcgcagga gccgccctgc agatcccctt tgccatgcag
     3421 atggcctatc ggttcaatgg catcggcgtg acccagaatg tgctgtacga gaaccagaag
     3481 ctgatcgcca atcagtttaa ctccgccatc ggcaagatcc aggactctct gagctccaca
     3541 gcaagcgccc tgggcaagct gcaggatgtg gtgaatcaga acgcccaggc cctgaacacc
     3601 ctggtgaagc agctgtctag caatttcggc gccatctcct ctgtgctgaa cgatatcctg
     3661 agccggctgg acaaggtgga ggcagaggtg cagatcgacc ggctgatcac aggcagactg
     3721 cagtccctgc agacctacgt gacacagcag ctgatcaggg cagcagagat cagggcatct
     3781 gccaacctgg cagcaaccaa gatgagcgag tgcgtgctgg gccagtccaa gagagtggac
     3841 ttttgtggca agggctatca cctgatgagc ttcccacagt ccgcccctca cggagtggtg
     3901 tttctgcacg tgacctacgt gccagcccag gagaagaact tcaccacagc accagcaatc
     3961 tgccacgatg gcaaggcaca ctttcctagg gagggcgtgt tcgtgagcaa tggcacccac
     4021 tggtttgtga cacagcgcaa cttctacgag ccacagatca tcaccacaga caatacattc
     4081 gtgtccggca actgtgacgt ggtcatcggc atcgtgaaca ataccgtgta tgatcctctg
     4141 cagccagagc tggactcttt taaggaggag ctggataagt acttcaagaa ccacaccagc
     4201 cccgacgtgg atctgggcga catctctggc atcaatgcca gcgtggtgaa catccagaag
     4261 gagatcgaca ggctgaatga ggtggccaag aatctgaacg agtccctgat cgatctgcag
     4321 gagctgggca agtatgagca gtacatcaag tggccctggt atatctggct gggcttcatc
     4381 gccggcctga tcgccatcgt gatggtgacc atcatgctgt gctgtatgac aagctgctgt
     4441 tcctgcctga agggctgctg ttcttgtggc agctgctgta agtttgatga ggacgatagc
     4501 gagcctgtgc tgaagggcgt gaagctgcac tacacctacc cctacgacgt gccagattat
     4561 gcctgaggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
     4621 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4681 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4741 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4801 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4861 ggcatgctgg ggatgcggtg ggctctatgg cttctactgg gcggttttat ggacagcaag
     4921 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
     4981 ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga
     5041 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5101 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5161 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5221 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     5281 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5341 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5401 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5461 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5521 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5581 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5641 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5701 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5761 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5821 catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct
     5881 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact
     5941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6001 tatccgctca tgagacaata accctgataa atgcttcaat aatagcacgt gctaaaactt
     6061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6781 aacgcggcct ttttacggtt cctgggcttt tgctggcctt ttgctcacat gttctt
//
