LOCUS       sequence-350093-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding JAM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: JAM2_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1001..2017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1941..1963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2025..2044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3149..3173)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3193..3216
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3251..3800
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3395..3412)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3622..3641
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3803..3805,3806..3808,3809..4522)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3803..3805,3806..3808,3809..4519)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3848..3869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4109..4128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4456..4477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4599..5187
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4652..4672)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5070..5081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5190..5206)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5191..5207)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5396..5629
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5701..5822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5738..5757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5792..5811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5862..5997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6017..6036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6018..6036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6059..6417)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6099..6120)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6311..6330
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6444..6548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6549..6617,6618..7409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6767..6786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7580..8168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8069..8088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8343..8646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8648..8846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8801..8821
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8864..9044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9091..9216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9713..9946
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10131..10175
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggggcgcg ccrggggggg sggggsgggg sgrggggsgg ggsggggsgr ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1021 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1081 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1141 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1201 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     1261 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1321 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1381 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1441 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1501 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1561 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     1621 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1681 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1741 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1801 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1861 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     1921 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1981 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     2041 tgtgctgtct catcattttg gcaaagaatt cccctagccg cggaatctag actgccggat
     2101 cgaattgtcg acgaattcgg atctggccaa tcacaagttt gtacaaaaaa gcaggctccg
     2161 cggccgcccc cttcacccct cctcagagca gccggctgcc gccccgggaa gatggcgagg
     2221 aggagccgcc accgcctcct cctgctgctg ctgcgctacc tggtggtcgc cctgggctat
     2281 cataaggcct atgggttttc tgccccaaaa gaccaacaag tagtcacagc agtagagtac
     2341 caagaggcta ttttagcctg caaaacccca aagaagactg tttcctccag attagagtgg
     2401 aagaaactgg gtcggagtgt ctcctttgtc tactatcaac agactcttca aggtgatttt
     2461 aaaaatcgag ctgagatgat agatttcaat atccggatca aaaatgtgac aagaagtgat
     2521 gcggggaaat atcgttgtga agttagtgcc ccatctgagc aaggccaaaa cctggaagag
     2581 gatacagtca ctctggaagt attagtggct ccagcagttc catcatgtga agtaccctct
     2641 tctgctctga gtggaactgt ggtagagcta cgatgtcaag acaaagaagg gaatccagct
     2701 cctgaataca catggtttaa ggatggcatc cgtttgctag aaaatcccag acttggctcc
     2761 caaagcacca acagctcata cacaatgaat acaaaaactg gaactctgca atttaatact
     2821 gtttccaaac tggacactgg agaatattcc tgtgaagccc gcaattctgt tggatatcgc
     2881 aggtgtcctg ggaaacgaat gcaagtagat gatctcaaca taagtggcat catagcagcc
     2941 gtagtagttg tggccttagt gatttccgtt tgtggccttg gtgtatgcta tgctcagagg
     3001 aaaggctact tttcaaaaga aacctccttc cagaagagta attcttcatc taaagccacg
     3061 acaatgagtg aaaatgattt caagcacaca aaatccttta taatttaaag actccacttt
     3121 agagatacac caaagggtgg gcgcgccgac ccagctttct tgtacaaagt ggtgggagga
     3181 ggtggatctg gcgactacaa ggacgacgat gacaagtgac tcgagcggga tcaattccgc
     3241 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3301 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3361 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3421 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3481 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3541 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3601 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3661 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3721 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3781 ctttgaaaaa cacgataata ccatggtgag caagggcgag gagctgttca ccggggtggt
     3841 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3901 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3961 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     4021 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4081 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4141 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4201 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4261 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4321 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4381 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     4441 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     4501 catggacgag ctgtacaagt aaagcggccg cgactctaga gtcgacctgc aggcatgcaa
     4561 gcttcaggta gccggctaac gttatcaagc ttatcgataa tcaacctctg gattacaaaa
     4621 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4681 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4741 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4801 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4861 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4921 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4981 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5041 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5101 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5161 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa
     5221 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5281 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     5341 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5401 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5641 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5821 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5941 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6001 ggacgtaccc aattcgccct atagtgagtc gtattagcat gtgagcaaaa ggccagcata
     6061 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6121 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6181 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6241 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6301 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6361 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7021 ctcgccttga tcgttgggaa ccgaagctga atgaagccat accaaacgac gagcgtgaca
     7081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     8281 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     8341 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     8401 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     8461 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     8521 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     8581 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     8641 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     8701 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     8761 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     8821 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     8881 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8941 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9001 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9061 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9121 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     9181 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9241 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9301 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9361 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9421 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9481 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9541 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9601 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9661 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9721 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9781 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9841 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     9901 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     9961 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10021 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10081 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10141 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10201 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10261 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10321 agacccacct cccaaccccg aggg
//
