LOCUS       sequence-350096-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding Rcan1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: Rcan1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1001
                     /label=chicken beta-actin promoter
     intron          1002..2018
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1942..1964
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2026..2045
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2134..2158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2797..2821)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2841..2864
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2899..3448
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3043..3060)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3270..3289
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3451..3453,3454..3456,3457..4170)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3451..3453,3454..3456,3457..4167)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3496..3517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3757..3776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4104..4125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4247..4835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4300..4320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4718..4729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4838..4854)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4839..4855)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5044..5277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5349..5470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5386..5405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5440..5459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5510..5645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5665..5684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5666..5684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5707..6065)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5747..5768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5959..5978
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6092..6196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6197..6265,6266..7057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6415..6434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7991..8294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8296..8494
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8449..8469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8512..8692
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8739..8864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9361..9594
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9779..9823
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggnsgcgc gccrggsggg gsggggsggg gsgrggggsg gggsggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     1021 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     1081 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     1141 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     1201 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     1261 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     1321 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     1381 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1441 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1501 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1561 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1621 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1681 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1741 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1801 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1861 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1921 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1981 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     2041 ttgtgctgtc tcatcatttt ggcaaagaat tcccctagcc gcggaatcta gactgccgga
     2101 tcgaattgtc gacgaattcg gatctggcca atcacaagtt tgtacaaaaa agcaggctcc
     2161 gcggccgccc ccttcaccat gcattttagg gactttagct acaattttag ctccctgatt
     2221 gcttgtgtgg caaacgatga tgtcttcagc gaaagtgaga ccagggccaa atttgaatcc
     2281 ctcttcagaa catatgacaa ggacaccacc ttccagtatt ttaagagctt caaacgtgtc
     2341 cggataaact tcagcaaccc cttatctgca gccgatgcca ggctgcggct gcacaagacc
     2401 gagttcctgg ggaaggaaat gaagttgtat tttgctcaga ctttacacat aggaagttca
     2461 cacctggctc cgcccaatcc cgacaaacag ttcctcatct cccctccggc ctctcctccc
     2521 gttggctgga aacaagtaga agatgccacc cccgtcataa attacgatct tttatatgcc
     2581 atctccaagc tggggccagg agagaagtat gaactgcatg cagcgacaga caccactccc
     2641 agtgtggtgg tccacgtgtg tgagagtgac caagagaatg aggaggaaga ggaagagatg
     2701 gagagaatga agagacccaa gcccaaaatc atccagacac ggagaccgga gtacacaccc
     2761 atccacctca gctgaaccgg aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     2821 tgggaggagg tggatctggc gactacaagg acgacgatga caagtgactc gagcgggatc
     2881 aattccgccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     2941 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3001 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3061 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     3121 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     3181 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3241 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     3301 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     3361 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     3421 tggttttcct ttgaaaaaca cgataatacc atggtgagca agggcgagga gctgttcacc
     3481 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3541 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3601 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3661 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3721 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3781 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3841 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3901 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     3961 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4021 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4081 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4141 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt cgacctgcag
     4201 gcatgcaagc ttcaggtagc cggctaacgt tatcaagctt atcgataatc aacctctgga
     4261 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4321 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4381 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4441 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4501 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4561 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4621 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     4681 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4741 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4801 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     4861 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4921 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4981 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5041 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5101 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5161 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5221 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5281 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5341 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5461 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5521 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5581 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5641 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attagcatgt gagcaaaagg
     5701 ccagcataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5761 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5821 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5881 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5941 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6001 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6061 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6121 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6181 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6241 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6301 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6361 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6421 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6481 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6541 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6601 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6661 tcatgtaact cgccttgatc gttgggaacc gaagctgaat gaagccatac caaacgacga
     6721 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6781 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6841 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6901 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6961 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7021 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7921 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     7981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     8041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     8101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     8161 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     8221 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     8281 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     8341 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     8401 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     8461 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
     8521 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8581 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8641 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8701 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8761 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8821 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8881 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8941 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9001 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9061 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9121 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9181 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     9241 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9301 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9361 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9421 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9481 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9541 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9601 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     9661 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9721 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9781 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9841 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9901 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9961 atcgtttcag acccacctcc caaccccgag gg
//
