LOCUS       sequence-350097-       10721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding GABPA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GABPA_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1001..2017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1941..1963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2025..2044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3526..3550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3570..3593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3628..4177
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3772..3789)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3999..4018
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4180..4182,4183..4185,4186..4899)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4180..4182,4183..4185,4186..4896)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4225..4246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4486..4505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4833..4854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4976..5564
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5029..5049)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5447..5458)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5567..5583)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5568..5584)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5773..6006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6078..6199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6115..6134)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6169..6188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6239..6374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6394..6413)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6395..6413)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6436..6794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6476..6497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6688..6707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6821..6925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6926..6994,6995..7786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7144..7163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7957..8545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8446..8465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8720..9023
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9025..9223
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9178..9198
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9241..9421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9468..9593
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10090..10323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10508..10552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggsgcgcg ccrggsgggg sggggsgggg sgrggggsgg ggsggggsga ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1021 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1081 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1141 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1201 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     1261 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1321 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1381 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1441 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1501 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1561 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     1621 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1681 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1741 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1801 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1861 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     1921 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1981 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     2041 tgtgctgtct catcattttg gcaaagaatt cccctagccg cggaatctag actgccggat
     2101 cgaattgtcg acgaattcgg atctggccaa tcacaagttt gtacaaaaaa gcaggcacca
     2161 tgactaaaag agaagcagag gagctgatag aaattgagat tgatggaaca gagaaagcag
     2221 agtgcacaga agaaagcatt gtagaacaaa cctacgcgcc agctgaatgt gtaagccagg
     2281 ccatagacat caatgaacca ataggcaatt taaagaaact gctagaacca agactacagt
     2341 gttctttgga tgctcatgaa atttgtctgc aagatatcca gctggatcca gaacgaagtt
     2401 tatttgacca aggagtaaaa acagatggaa ctgtacagct tagtgtacag gtaatttctt
     2461 accaaggaat tgaaccaaag ttaaacatcc ttgaaattgt taaacctgcg gacactgttg
     2521 aggttgttat tgatccagat gcccaccatg ctgaatcaga agcacatctt gttgaagaag
     2581 ctcaagtgat aactcttgat ggcacaaaac acatcacaac catttcagat gaaacttcag
     2641 aacaagtgac aagatgggct gctgcactgg aaggctatag gaaagaacaa gaacgccttg
     2701 ggatacccta tgatcccata cagtggtcca cagaccaagt cctgcattgg gtggtttggg
     2761 taatgaagga attcagcatg accgatatag acctcaccac actcaacatt tcggggagag
     2821 aattatgtag tctcaaccaa gaagattttt ttcagcgggt tcctcgggga gaaattctct
     2881 ggagtcatct ggaacttctc cgaaaatatg tattggcaag tcaagaacaa cagatgaatg
     2941 aaatagttac aattgatcaa cctgtgcaaa ttattccagc atcagtgcaa tctgctacac
     3001 ctactaccat taaagttata aatagtagtg cgaaagcagc caaagtacaa agagcgccga
     3061 ggatttcagg agaagataga agctcacctg ggaacagaac aggaaacaat ggccaaatcc
     3121 aactatggca gtttttgcta gaacttctta ctgataagga cgctcgagac tgcatttctt
     3181 gggttggtga tgaaggtgaa tttaagctaa atcagcctga actggttgca cagaaatggg
     3241 gacagcgtaa aaataagcct acgatgaact atgagaaact cagtcgtgca ttaagatatt
     3301 attacgatgg ggacatgatt tgtaaagttc aaggcaagag atttgtgtac aagtttgtct
     3361 gtgacttgaa gactcttatt ggatacagtg cagcggagtt gaaccgtttg gtcacagaat
     3421 gtgaacagaa gaaacttgca aagatgcagc tccatggaat tgcccagcca gtcacagcag
     3481 tagctctgtc tactgcttct ctgcaaacgg aaaaggataa ttagaaccca gctttcttgt
     3541 acaaagtggt gggaggaggt ggatctggcg actacaagga cgacgatgac aagtgactcg
     3601 agcgggatca attccgcccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     3661 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     3721 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3781 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3841 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3901 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3961 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4021 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4081 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4141 acggggacgt ggttttcctt tgaaaaacac gataatacca tggtgagcaa gggcgaggag
     4201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4381 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4861 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagagtc
     4921 gacctgcagg catgcaagct tcaggtagcc ggctaacgtt atcaagctta tcgataatca
     4981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5341 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     5581 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5641 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5701 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5761 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5821 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6001 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6061 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6181 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6301 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6361 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttagcatgtg
     6421 agcaaaaggc cagcataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6541 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6601 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6781 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7141 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7381 catgggggat catgtaactc gccttgatcg ttgggaaccg aagctgaatg aagccatacc
     7441 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8101 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8581 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8641 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     8701 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8761 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     8821 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8881 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8941 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9001 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     9061 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     9121 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     9181 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     9241 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9301 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9361 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9421 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9481 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9541 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9601 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9661 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9721 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9781 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9841 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9901 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     9961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    10021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    10081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg g
//
