LOCUS       sequence-350098-       13136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding TRAPPC10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: TRAPPC10_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..13136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1000
                     /label=chicken beta-actin promoter
     intron          1001..2017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1941..1963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2025..2044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5941..5965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5985..6008
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6043..6592
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6187..6204)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6414..6433
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6595..6597,6598..6600,6601..7314)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6595..6597,6598..6600,6601..7311)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6640..6661)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6901..6920)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7248..7269
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7391..7979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7444..7464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7862..7873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7982..7998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7983..7999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8188..8421
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8493..8614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8530..8549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8584..8603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8654..8789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8809..8828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8810..8828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(8851..9209)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8891..8912)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9103..9122
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9236..9340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9341..9409,9410..10201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9559..9578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10372..10960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10861..10880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        11135..11438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11440..11638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11593..11613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11656..11836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11883..12008
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12505..12738
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12923..12967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggsgcgcg ccrggsgggg sggggsgggg sgrggggsgg ggsggggcga ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1021 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1081 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1141 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1201 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     1261 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1321 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1381 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1441 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1501 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1561 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     1621 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1681 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1741 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1801 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1861 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     1921 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1981 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     2041 tgtgctgtct catcattttg gcaaagaatt cccctagccg cggaatctag actgccggat
     2101 cgaattgtcg acgaattcgg atctggccaa tcacaagttt gtacaaaaaa gcaggcacca
     2161 tggacgcctc tgaggagccg ctgccgccgg tgatctacac catggagaac aagcccatcg
     2221 tcacctgtgc tggagatcag aatttattta cctctgttta tccaacgctc tctcagcagc
     2281 ttccaagaga accaatggaa tggagaaggt cctatggccg ggctccgaag atgattcacc
     2341 tagagtctaa ctttgttcaa ttcaaagagg agctgctgcc caaagaagga aacaaagctc
     2401 tgctcacgtt tcccttcctc catatttact ggacagagtg ctgtgatacc gaagtgtata
     2461 aagctacagt aaaagatgac ctcaccaagt ggcagaatgt tctgaaggct catagctctg
     2521 tggactggtt aatagtgata gttgaaaatg atgccaagaa aaaaaacaaa accaacatcc
     2581 ttccccgaac ctctattgtg gacaaaataa gaaatgattt ttgtaataaa cagagtgaca
     2641 ggtgtgttgt gctctccgac cccttgaagg actcttctcg aactcaggaa tcctggaatg
     2701 ccttcctgac caaactcagg acattgcttc ttatgtcttt taccaaaaac ctaggcaagt
     2761 ttgaggatga catgagaacc ttgagggaga agaggactga gccaggctgg agcttttgtg
     2821 aatatttcat ggttcaggag gagcttgcct ttgttttcga gatgctgcag cagttcgagg
     2881 acgccctggt gcagtacgac gaactggacg ccctcttctc tcagtatgtg gtcaacttcg
     2941 gggccgggga tggtgccaac tggctgactt ttttctgcca gccagtgaag agctggaacg
     3001 gattgatcct ccgaaaaccc atagatatgg agaagcggga atcgatccag aggcgagaag
     3061 ccaccctgtt agatctgcgc agttacctgt tctctcgcca gtgcaccttg ctgctcttcc
     3121 tgcagaggcc gtgggaggtg gcccagcgcg ccctagagct gctgcacaac tgcgtgcagg
     3181 aactgaagct cttagaagtc tctgtcccac ctggtgctct ggactgctgg gtgtttctga
     3241 gctgtctgga ggtgttgcag aggatagaag gctgctgtga ccgggcacag atcgactcaa
     3301 acattgccca cactgtgggg ctatggagct atgccacaga aaagttaaag tccttgggct
     3361 atctatgtgg acttgtgtca gagaaaggac ctaactcaga agatctcaac aggacagttg
     3421 accttttggc aggtttggga gctgagcgac cagaaacagc caacacagct cagagtcctt
     3481 ataagaaact gaaagaagca ttatcgtcag tggaagcttt tgaaaaacac tacttagatt
     3541 tgtcccatgc caccattgaa atgtatacaa gcattgggag gattcgatct gctaagtttg
     3601 ttggaaaaga tctggcagag ttttacatga ggaaaaaggc tccacaaaag gcagaaatct
     3661 atcttcaagg agcactgaaa aactacctgg ctgagggctg ggcactcccc atcacacaca
     3721 caaggaagca gctggccgaa tgtcaaaagc accttggaca aattgaaaac tacctgcaga
     3781 ccagcagcct cttagccagt gaccaccacc tcactgaaga ggagcgcaag cacttctgcc
     3841 aggagatact tgactttgcc agccagccgt cagacagccc aggtcataag atagtgctac
     3901 ccatgcattc ctttgcacaa ctgcgagatc tccattttga tccctccaat gccgtggtcc
     3961 acgtgggcgg cgttttgtgc gttgagataa ccatgtacag ccagatgcct gtgcctgttc
     4021 acgtggagca gattgtggtc aatgtccact tcagcattga gaaaaacagc taccggaaga
     4081 ctgcggagtg gcttaccaag cacaagacgt ccaatgggat cattaacttt ccacccgaga
     4141 ccgcaccttt ccctgtatcc caaaacagtt tgcccgcgct ggagttgtat gaaatgtttg
     4201 agagaagccc atctgataac tccttgaaca cgactgggat tatctgcaga aacgtccaca
     4261 tgctcctgag aaggcaggag agcagctcct ctctagagat gccctcaggg gtggctctgg
     4321 aggagggtgc ccacgtgctg aggtgcagcc acgtgaccct ggaaccaggg gccaaccaga
     4381 taacattcag gactcaggcc aaggaacctg gaacgtatac actcaggcag ctgtgcgcct
     4441 cggtgggctc cgtgtggttc gtcctccctc acatctaccc cattgtgcag tacgacgtgt
     4501 actcacagga gccccagctg cacgtggagc cgctggctga tagccttctg gcaggcattc
     4561 ctcagagagt caagttcact gtcactaccg gccattatac gataaagaat ggagacagcc
     4621 tgcagcttag caatgccgaa gccatgctca tcctgtgcca ggcggagagc agggctgtgg
     4681 tctactccaa cacgagagaa cagtcttctg aggccgcgct ccggattcag tcctccgaca
     4741 aggtcacgag catcagtctg cctgttgcgc ctgcgtacca cgtgatcgaa tttgaactgg
     4801 aagttctctc tttaccttca gccccagcac tcggagggga gagtgacatg ctggggatgg
     4861 cagagcccca caggaagcat aaggacaaac agagaactgg ccgctgcatg gttaccacag
     4921 accacaaagt gtcgattgac tgcccgtggt ccatctactc cacagtcatc gcactgacct
     4981 tcagcgtacc cttcaggacc acacacagcc tcctgtcctc aggaacacgg aaatatgttc
     5041 aagtttgtgt ccagaatttg tcagaacttg actttcagct gtcagatagt tatcttgtag
     5101 ataccggtga tagtaccgac ctgcaactag taccactgaa cacgcagtcc cagcagccca
     5161 tctacagcaa gcagtcggtg ttcttcgtct gggaactcaa gtggacagaa gagcctcccc
     5221 cttctctgca ttgccggttc tctgttggat tttccccagc ttctgaggaa cagctgtcta
     5281 tctccttaaa gccgtatact tatgaattta aagtggaaaa tttttttaca ttatacaacg
     5341 tgaaggctga gatctttccc ccttcgggaa tggagtattg cagaacaggc tccctctgct
     5401 ccctggaggt tttgatcacg aggctctcag acctcttgga ggtggataaa gatgaagcac
     5461 tgactgaatc tgatgagcat ttttcgacaa agcttatgta tgaagttgtc gacaacagta
     5521 gcaactgggc agtgtgtggg aaaagctgcg gtgtcatctc catgccagtg gctgctcggg
     5581 ccactcacag ggtccacatg gaagtgatgc cgctcttcgc cgggtatctc cccctgcccg
     5641 acgtcaggct gttcaagtac ctcccccatc attctgcaca ctcctcccaa ctggacgctg
     5701 acagctggat agaaaacgac agcctgtcag tagacaagca cggggacgac cagccggaca
     5761 gcagcagcct caagagcagg ggcagcgtgc attcggcctg cagcagcgag cacaaaggcc
     5821 tacccatgcc ccggctgcag gcactgccgg ccggccaggt cttcaactcc agctcgggca
     5881 cacaagtcct ggtcatcccc agccaagatg accacgtcct ggaagtcagt gtaacataga
     5941 acccagcttt cttgtacaaa gtggtgggag gaggtggatc tggcgactac aaggacgacg
     6001 atgacaagtg actcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc
     6061 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     6121 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     6181 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     6241 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     6301 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     6361 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6421 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6481 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6541 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtg
     6601 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     6661 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     6721 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     6781 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     6841 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     6901 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6961 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     7021 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     7081 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     7141 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     7201 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     7261 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     7321 cgcgactcta gagtcgacct gcaggcatgc aagcttcagg tagccggcta acgttatcaa
     7381 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7801 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7981 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     8041 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     8101 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     8161 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8221 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8281 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8341 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8401 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8461 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8521 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8581 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8641 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8701 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8761 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8821 tcgtattagc atgtgagcaa aaggccagca taaatcaaaa gaatagaccg agatagggtt
     8881 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8941 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9001 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9061 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9121 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9181 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     9241 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9301 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9361 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9421 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9481 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9541 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9601 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9661 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9721 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9781 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccgaagct
     9841 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9901 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9961 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10021 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10081 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10141 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10201 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10261 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10321 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10381 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10441 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10501 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10561 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10621 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10681 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10741 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10801 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10861 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10921 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10981 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11041 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
    11101 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    11161 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    11221 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    11281 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    11341 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
    11401 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
    11461 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
    11521 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
    11581 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
    11641 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    11701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    11761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    11821 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    11881 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    11941 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    12001 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    12061 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    12121 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    12181 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    12241 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    12301 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
    12361 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    12421 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    12481 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    12541 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    12601 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    12661 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    12721 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    12781 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    12841 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    12901 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    12961 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    13021 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    13081 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggg
//
