LOCUS       sequence-35015-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: p38delta
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc caatgccagc ctcactcgga
      121 aaaggggctt ctacaagcag gacatcaaca agactgcctg ggagctaccc aagacctacc
      181 tggcgcccgc gcacgtcggc agcggggcct atggagcggt gtgctcggcc atcgacaagc
      241 ggacagggga gaaggtggcc atcaagaagc tgagccggcc cttccagtcg gagatctttg
      301 ccaagcgcgc ttaccgcgag cttctgctct tgaagcacat gcaccatgag aacgtcattg
      361 ggcttctgga tgtcttcacc cctgcgtctt cccttcggag cttccatgat ttctacctgg
      421 tgatgccctt catgcagaca gacctacaga agatcatggg gatggaattc agcgaggata
      481 aggtccagta cttggtgtac cagatgctca aaggtctaaa gtacatccac tccgctggca
      541 tcgtccacag ggacctgaaa ccgggcaacc tggctgtgaa tgaagactgt gagctgaaga
      601 tcctggactt tgggctggcc cgccacacag acactgagat gacgggctat gtggtgaccc
      661 gctggtaccg ggcccccgag gtgatcctca gctggatgca ttacaaccag acagtcgaca
      721 tctggtctgt tggttgcatc atggcagaaa tgctgactgg aaagacactc ttcaagggca
      781 aggactacct ggaccagctg acccagatcc tgaaagtgac tggggtgcca ggtgccgagt
      841 tcgtgcagaa gctgaaagac aaggcggcca aatcctatat tcagtccctg ccccagagcc
      901 ccaagaa
//
