LOCUS       sequence-350174-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of nuclear-localized dTomato with 1 high 
            affinty MS2 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Hung ME, Leonard JN
  TITLE     A platform for actively loading cargo RNA to elucidate limiting 
            steps in EV-mediated delivery.
  JOURNAL   J Extracell Vesicles. 2016 May 13;5:31027. doi: 
            10.3402/jev.v5.31027. eCollection 2016.
  PUBMED    27189348
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Extracell
            Vesicles."; date: "2016-05-13"; volume: "5:31027. doi"; pages: " 
            10.3402/jev.v5.31027. eCollection 2016"
COMMENT     Sequence Label: pMEH294 NLS dTomato 1x high affinity MS2 loop
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             99..732
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    779..904
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1401..1634
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1819..1863
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2161..2278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2343..2717)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2714..2723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2727..2745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2737..2784)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2889..3192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3193..3396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3347..3367
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3393..3417
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3498..3518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3528..4229
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3661..3679)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3929..3948
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4123..4146
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4239..4827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4292..4312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4710..4721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_RNA        4850..4868
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     LTR             4941..5174
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5275..5292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5281..5505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5551..5979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5638..5657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5848..5869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5988..6008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5993..6322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6173..6308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6235..6254
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6371..7396
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6371..7396
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7526..7647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7696..7712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7696..7712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7709..7731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7720..7736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7744..7750,7751..7768,7769..7774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7789..7810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7927..7944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8098..8686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8178..8197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8857..9648,9649..9717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9480..9499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9718..9822)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9870..10004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
       61 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
      121 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
      181 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
      241 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
      301 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
      361 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
      421 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
      481 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
      541 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      721 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      781 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      841 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      901 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
      961 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1021 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1081 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1141 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1201 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     1261 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1321 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1381 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1441 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1501 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1561 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1621 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1681 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1741 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1801 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1861 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1921 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1981 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2041 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2101 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     2161 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2221 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2281 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc tctagtccgg
     2341 aatcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg
     2401 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg
     2461 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac
     2521 acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc
     2581 acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc
     2641 cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca
     2701 ctggtcaact tggccatggt ggccctccta tagtgagtcg tattatacta tgccgatata
     2761 ctatgccgat gattaattgt caacacgtgc tgcaggtccg aggttctaga cgtattaccg
     2821 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2881 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2941 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3001 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3061 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3121 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3181 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3241 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3301 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3361 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3421 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta ctagaggatc
     3481 tgccaccagc tagcatgccc aagaaaaagc ggaaggtgtc ccggggaatg gtgagcaagg
     3541 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     3601 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3661 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     3721 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     3781 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     3841 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     3901 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3961 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     4021 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     4081 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     4141 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     4201 tcctgtacgg catggacgag ctgtacaagt aggtcgacaa tcaacctctg gattacaaaa
     4261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4621 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4741 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4801 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacaaa catgaggatc
     4861 acccatgtcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4921 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4981 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5041 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5101 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5161 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5221 gaaatgaata tcagagagtg agaggccttg acattgttta aacccgctga tcagcctcga
     5281 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5341 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5401 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5461 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5521 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5581 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5641 ctttcgcttt cttcccttcc tttctcgtca cgttcgccgg ctttccccgt caagctctaa
     5701 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5761 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5821 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5881 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5941 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6001 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6061 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6241 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6301 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6361 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     6421 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     6481 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     6541 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     6601 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     6661 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     6721 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     6781 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     6841 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     6901 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     6961 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7021 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7081 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     7141 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     7201 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     7261 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     7321 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     7381 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7441 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7501 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7621 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9841 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
     9901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10021 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aat
//
