LOCUS       sequence-350175-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of nuclear-localized dTomato with 1 wild type 
            MS2 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Hung ME, Leonard JN
  TITLE     A platform for actively loading cargo RNA to elucidate limiting 
            steps in EV-mediated delivery.
  JOURNAL   J Extracell Vesicles. 2016 May 13;5:31027. doi: 
            10.3402/jev.v5.31027. eCollection 2016.
  PUBMED    27189348
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Extracell
            Vesicles."; date: "2016-05-13"; volume: "5:31027. doi"; pages: " 
            10.3402/jev.v5.31027. eCollection 2016"
COMMENT     Sequence Label: pMEH295 NLS dTomato 1x wild type MS2 loop
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             10..643
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1312..1545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1730..1774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2072..2189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2254..2628)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2625..2634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2638..2656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2648..2695)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2800..3103
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3104..3307
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3258..3278
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3304..3328
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3409..3429
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3439..4140
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3572..3590)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3840..3859
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4034..4057
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4150..4738
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4203..4223)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4621..4632)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4852..5085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5186..5203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5192..5416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5462..5890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5549..5568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5759..5780
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5899..5919)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5904..6233
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6084..6219
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6146..6165
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6282..7307
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6282..7307
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7437..7558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7607..7623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7607..7623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7620..7642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7631..7647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7655..7661,7662..7679,7680..7685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7700..7721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7838..7855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8009..8597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8089..8108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8768..9559,9560..9628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9391..9410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9629..9733)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9781..9915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
       61 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
      121 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
      181 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
      241 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
      301 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
      361 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
      421 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1201 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1261 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1321 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1381 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1441 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1501 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1561 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1621 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1681 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1741 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     1801 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     1861 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     1921 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     1981 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     2041 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2101 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2161 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2221 tttggttagt accgggcccg ctctagtccg gaatcagtcc tgctcctcgg ccacgaagtg
     2281 cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat
     2341 ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc
     2401 ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac
     2461 ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc
     2521 gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc
     2581 gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg tggccctcct
     2641 atagtgagtc gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg
     2701 ctgcaggtcc gaggttctag acgtattacc gccatgcatt agttattaat agtaatcaat
     2761 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2821 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2881 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2941 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3001 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3061 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3121 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     3181 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     3241 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     3301 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     3361 ccatagaaga caccgactct actagaggat ctgccaccag ctagcatgcc caagaaaaag
     3421 cggaaggtgt cccggggaat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     3481 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     3541 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     3601 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     3661 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     3721 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     3781 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     3841 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     3901 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     3961 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     4021 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4081 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     4141 taggtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4321 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4381 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4441 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4501 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4561 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4621 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4681 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4741 ggaattcgag ctcggtacaa acatgaggat tacccatgtc ggtaccttta agaccaatga
     4801 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     4861 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5101 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     5161 gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5221 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5281 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5341 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5401 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5461 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5521 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgtc
     5581 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5641 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5701 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5761 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5821 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5881 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6001 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6061 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6121 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6181 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6241 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
     6301 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6361 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6421 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6481 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6541 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6601 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6661 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6721 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6781 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6841 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     6901 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     6961 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     7021 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     7081 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7141 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7201 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7261 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag
     7321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7561 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9781 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9841 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9901 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9961 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10021 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaa
//
