LOCUS       sequence-350178-        3732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning 
            ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK035
FEATURES             Location/Qualifiers
     source          1..3732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaatgatga ccttgctcaa tggccacgcc tccctctccc cggccgctca
     2281 acgccgcatc gaacaccatg aagctgatat tgtaattgtg ggtgccggag tcctcggctg
     2341 tgcacttgca gttgcactgg gaaatcaagg gcgcagtgta atcctgctgg agaagtcgtt
     2401 ggaagagccc aaccgtattg ttggtgaact actccaaccg ggaggtgttc aggcgctcga
     2461 gcaactaggg ctacgggatt gtctggaaga tatcgatggc atcaatgtga agggatactg
     2521 ggttacttac tttggggagc cggttctcct cgaatacccc aagtccagcc caacctcgcc
     2581 tacaccgttg ggacgagcct tccaccatgg ccgattcgtg atgaaactac gggcagccgc
     2641 tctgtcgtgc cccaatgtca cggtggtcga aaccaaggtt accggcctca tcacatcctc
     2701 atatactcag gaagtccttg gagtcgagtg tataaccaag gatgtcaagg actgctactt
     2761 cggtcaactc accgtggccg ccgacggtta taactccgtt ttccgcaagg aacaccacca
     2821 gtatacaccc aaacggcgct caaagttcca tggcttggag ctcatcgatg ctaaactgcc
     2881 cgctcccaac actggtcatg tgcttctcag cgataatcca cccgtactta tgtaccagat
     2941 cggcacccat gaaacccgaa tccttatcga tatccctgat aacctgcctg cggcctcggt
     3001 caagaacggt ggtgtcaagg gttacatgcg aaacaacatc ctgcccaagc tcccagaggg
     3061 tgtccagcag tcgttctccg atgccctaga gaagggccag ctacggtcaa tgccgaactc
     3121 gttcctgcct gcctcggcca acaagacacc cggcttgatg attctgggcg atgcgcttaa
     3181 catgcggcac cctctcacag gtggaggcat gactgtggcg ctcaacgatg tttgtgtcat
     3241 ccgcgaattg ctcagcccag agcgcgtgcc caacctctcc aatacaggcc ttgttctcga
     3301 gcagctcgca gagttccact ggaaacgaaa gaactcatct tcggtcatca atatcctcgc
     3361 acaggctctc tatgcgctct tcgctgcaga cagtacgcac gctcactctt caggttaatt
     3421 tgcaataact aactgttcat tcaacagact attacctcaa agctcttcag cgtggctgtt
     3481 tccgctactt ccagattgga ccagttggcg gccctgtcgg gttgttagcc ggtctaatca
     3541 agaaacccct cgtccttgtc agccacttct tctccgttgc cttcctcgcc atctgggtcc
     3601 acatatgcga tacccctctg tccaagctat tcctagcccc atactacgcc gtcatgatat
     3661 tgtacacagc ctctgtcgtg attttgccat acatctggac cgagatctgg tactgagctt
     3721 tgagaccacg aa
//
